SP65_DICDI
ID SP65_DICDI Reviewed; 464 AA.
AC Q9NAX4; Q54YL6;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 82.
DE RecName: Full=Spore coat protein SP65;
DE Short=Spore coat protein E;
DE Flags: Precursor;
GN Name=cotE; Synonyms=SP65; ORFNames=DDB_G0277903;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, DEVELOPMENTAL
RP STAGE, AND INTERACTION WITH SP85.
RX PubMed=17416892; DOI=10.1128/ec.00329-06;
RA Metcalf T., van der Wel H., Escalante R., Sastre L., West C.M.;
RT "Role of SP65 in assembly of the Dictyostelium discoideum spore coat.";
RL Eukaryot. Cell 6:1137-1149(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP PROTEIN SEQUENCE OF 18-47 AND 166-180, AND INTERACTION WITH SP85.
RX PubMed=10564654; DOI=10.1242/jcs.112.23.4367;
RA Zhang Y., Zhang P., West C.M.;
RT "A linking function for the cellulose-binding protein SP85 in the spore
RT coat of Dictyostelium discoideum.";
RL J. Cell Sci. 112:4367-4377(1999).
CC -!- FUNCTION: Forms a triad with cellulose and pspB that is essential for
CC spore outer layer formation.
CC -!- SUBUNIT: Binds to the C-terminal region of pspB.
CC -!- INTERACTION:
CC Q9NAX4; P54704: pspB; NbExp=2; IntAct=EBI-1808756, EBI-1808765;
CC -!- SUBCELLULAR LOCATION: Spore wall {ECO:0000269|PubMed:17416892}.
CC Note=Depends on pspB for incorporation into the coat. Accumulates
CC initially in vesicles distinct from prespore vesicles (PSVs).
CC -!- DEVELOPMENTAL STAGE: Expressed at or just prior to spore coat
CC formation, at 20 to 22 hours of development of the fruiting bodies.
CC Secreted to the cell surface before cellulose is deposited.
CC {ECO:0000269|PubMed:17416892}.
CC -!- DOMAIN: The N-terminal part (18-235) contains the information required
CC for insertion into the coat.
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DR EMBL; AF279135; AAF82380.1; -; Genomic_DNA.
DR EMBL; AAFI02000023; EAL68124.1; -; Genomic_DNA.
DR RefSeq; XP_642186.1; XM_637094.1.
DR AlphaFoldDB; Q9NAX4; -.
DR IntAct; Q9NAX4; 1.
DR STRING; 44689.DDB0214991; -.
DR PaxDb; Q9NAX4; -.
DR EnsemblProtists; EAL68124; EAL68124; DDB_G0277903.
DR GeneID; 8621393; -.
DR KEGG; ddi:DDB_G0277903; -.
DR dictyBase; DDB_G0277903; cotE.
DR HOGENOM; CLU_589780_0_0_1; -.
DR InParanoid; Q9NAX4; -.
DR OMA; INTRSCC; -.
DR PRO; PR:Q9NAX4; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0031410; C:cytoplasmic vesicle; IDA:dictyBase.
DR GO; GO:0031160; C:spore wall; IDA:dictyBase.
DR GO; GO:0005199; F:structural constituent of cell wall; IMP:dictyBase.
DR GO; GO:0042244; P:spore wall assembly; IMP:dictyBase.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IMP:dictyBase.
DR InterPro; IPR003645; Fol_N.
DR SMART; SM00274; FOLN; 3.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycoprotein; Reference proteome; Repeat;
KW Signal; Sporulation.
FT SIGNAL 1..17
FT /evidence="ECO:0000269|PubMed:10564654"
FT CHAIN 18..464
FT /note="Spore coat protein SP65"
FT /id="PRO_0000327782"
FT DOMAIN 21..43
FT /note="Follistatin-like 1"
FT DOMAIN 121..143
FT /note="Follistatin-like 2"
FT DOMAIN 151..173
FT /note="Follistatin-like 3"
FT REGION 250..320
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 111
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 247
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 44
FT /note="Missing (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 464 AA; 47250 MW; E97723B13144C445 CRC64;
MKVLLLLVCL VFAYVNASYD ACYNVVCPSN YQCRAEGDQA YCVPEHHEYG CDRHSCGRGY
ECVERWDSFC CKPIHHRPHP HPRPPHPHPR PTNCDYTSCP REFDCHVINR NVTACLPDNH
VCRDFQCPVG THCFNGERGP HCVSDTHYPN LCRVTKCSYD FTCKMVRGNP TCLRNHDGIS
THSPTHTPTH SPTTSPTHCP SCTELAPFCH SAGLVCRTIL NPSCVSAVRG IINTRSCCTY
IAVCASNSTS TTGATTGATT PTSTTGAATT AAATTTTATS TTGAATTAPA TTTTTSTTGA
ATTAPATTTT STTGAATTAP ATTTTTATTI VGVTTANSVG GLTTTRATTV AGVTTANSVG
GLTTAGITTV AGATTGNSVG GLTTARATTV ADVTTANSVG GLTTAGVTTV AGATTGNSVG
GLTTARATTV ADVTTANSVG GLTTSRATTI AGATTANSIG GLTN
