ID   SP87_DICDI              Reviewed;         677 AA.
AC   P54643; Q54ZQ8; Q75J88;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 101.
DE   RecName: Full=Spore coat protein SP87;
DE   AltName: Full=Protein PL3;
DE   Flags: Precursor;
GN   Name=pspD; ORFNames=DDB_G0277379;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=AX3;
RX   PubMed=8174787; DOI=10.1006/dbio.1994.1122;
RA   Yoder B.K., Mao J., Erdos G.W., West C.M., Blumberg D.D.;
RT   "Identification of a new spore coat protein gene in the cellular slime mold
RT   Dictyostelium discoideum.";
RL   Dev. Biol. 163:49-65(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: May contribute to the structure of the coat at the interface
CC       between the middle, cellulosic layer and the outer, electron-dense,
CC       proteinaceous layer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle. Spore
CC       coat. Note=Accumulates specifically in regulated secretory vesicles of
CC       prespore cells (prespore vesicles). The protein later accumulates
CC       extracellularly in the spore coat.
CC   -!- INDUCTION: By cAMP.
CC   -!- PTM: Disulfide bonding is important for associating SP87 with the coat.
CC   -!- PTM: Phosphorylated on serine residues.
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DR   EMBL; U25144; AAA73515.1; -; Genomic_DNA.
DR   EMBL; AAFI02000019; EAL68746.1; -; Genomic_DNA.
DR   RefSeq; XP_642678.1; XM_637586.1.
DR   AlphaFoldDB; P54643; -.
DR   STRING; 44689.DDB0191310; -.
DR   PaxDb; P54643; -.
DR   EnsemblProtists; EAL68746; EAL68746; DDB_G0277379.
DR   GeneID; 8620997; -.
DR   KEGG; ddi:DDB_G0277379; -.
DR   dictyBase; DDB_G0277379; pspD.
DR   eggNOG; ENOG502RGEP; Eukaryota.
DR   HOGENOM; CLU_406241_0_0_1; -.
DR   InParanoid; P54643; -.
DR   OMA; ETTSYCA; -.
DR   PhylomeDB; P54643; -.
DR   PRO; PR:P54643; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0031012; C:extracellular matrix; HDA:dictyBase.
DR   GO; GO:0031160; C:spore wall; IDA:dictyBase.
DR   GO; GO:0030133; C:transport vesicle; IEA:UniProtKB-SubCell.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEP:dictyBase.
DR   InterPro; IPR007643; Dict_spore_N.
DR   InterPro; IPR003645; Fol_N.
DR   Pfam; PF04562; Dicty_spore_N; 1.
DR   SMART; SM00274; FOLN; 9.
PE   2: Evidence at transcript level;
KW   Cytoplasmic vesicle; Disulfide bond; Glycoprotein; Reference proteome;
KW   Repeat; Signal; Sporulation.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..677
FT                   /note="Spore coat protein SP87"
FT                   /id="PRO_0000032672"
FT   DOMAIN          22..142
FT                   /note="DSCP-N"
FT   DOMAIN          188..210
FT                   /note="Follistatin-like 1"
FT   DOMAIN          231..253
FT                   /note="Follistatin-like 2"
FT   DOMAIN          275..298
FT                   /note="Follistatin-like 3"
FT   DOMAIN          315..338
FT                   /note="Follistatin-like 4"
FT   DOMAIN          355..378
FT                   /note="Follistatin-like 5"
FT   DOMAIN          418..440
FT                   /note="Follistatin-like 6"
FT   DOMAIN          479..501
FT                   /note="Follistatin-like 7"
FT   DOMAIN          515..535
FT                   /note="Follistatin-like 8"
FT   DOMAIN          571..593
FT                   /note="Follistatin-like 9"
FT   REGION          148..184
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        72
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   677 AA;  71805 MW;  EF6E29CFF57E78D5 CRC64;
     MLFLKNIGVF FMIFLVSKSY ATDCNKITNE EECHKSSECI VINYTPCCGE QKWACSKGTF
     DTCTYENSCY RNSSNNQVVE VSNKCFNLDG FIKITTPTEY SCSDAKIKEC ALLGKSCSFQ
     KNSCSNPTSC CPGESICEGL SSGSSTSGGG SSGGTSGGSS SGGTSGGSSS GGTSGSSSSG
     SSSGGVSSCS TTHCPEGYHC SMVNDVATCL ASTTGGTGLP GTSSSTAGVS SCLTTLCPIG
     HICVEDSNGV NCVPNGGGTS GGSSSTGTSG GHPDPCRDVD CPDGFHCECK DGKTAKCVPS
     PTTGSSSTSG GHPDPCKDVT CPDGFHCECK DGKTAKCVPS PTTGSSSTSG GNTNPCSNVN
     CPDGFYCECK DGKTAKCVPS GPTQPPKPPV CSLRCPPNHE CRFNDQGHQC CVKVHHDRCS
     LRCPHGHECK VDHNGKECCV RSHRPPPPEV CSLRCPPKHE CKFDDHGKKC CVKIHCDEVC
     DLDCGRGFEC KIRHDGSKCC VRSERPHPPQ HEKCNKRCPP GHECKVDQHG KECCVVAHRP
     PPKCSLRCPP RHECRVNHFG EECCVKVHHD KCSLRCPPGH ECKVDQHGKE CCVVAHRPPP
     KCSLRCPPKH ECRINHFGEE CCVKSRNDCL TCEDLNCERK GLHCAMKTVP IDKENCCEKV
     PVCYSTNPLL DGGHGFI