SP8_CHICK
ID SP8_CHICK Reviewed; 480 AA.
AC Q64HY5;
DT 13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Transcription factor Sp8;
GN Name=SP8;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=15358670; DOI=10.1242/dev.01331;
RA Kawakami Y., Rodriguez Esteban C., Matsui T., Rodriguez-Leon J., Kato S.,
RA Izpisua Belmonte J.C.;
RT "Sp8 and Sp9, two closely related buttonhead-like transcription factors,
RT regulate Fgf8 expression and limb outgrowth in vertebrate embryos.";
RL Development 131:4763-4774(2004).
CC -!- FUNCTION: Transcription factor which plays a key role in limb
CC development. Positively regulates FGF8 expression in the apical
CC ectodermal ridge (AER) and contributes to limb outgrowth in embryos.
CC {ECO:0000269|PubMed:15358670}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Detected in early stages of embryos as an oval and
CC 2 stripes at HH stage 5, which becomes two lateral stripes at HH stage
CC 7, running along the anteroposterior body axis from the head ectoderm
CC to the anterior region of the primitive streak. At HH stage 8, strong
CC expression in the anterior neuroectoderm, which forms the central
CC nervous system, is observed, and at HH stage 9, expressed in the most
CC anterior region of the forebrain, midbrain, neural groove and Hensen's
CC node. At HH stage 15-16, the expression is seen in the surface ectoderm
CC of the limb-forming fields, in addition to the neural tube. The
CC expression in the limb field became confined to the distal region of
CC the limb at HH stage 16 and 17, with still scattered signal visible in
CC the surface ectoderm of the limb bud. Strongly expressed in the apical
CC ectodermal ridge (AER) and weakly in the ectoderm in the developing
CC limb bud at HH stage 21. The expression in the AER is detected
CC throughout later stages of limb development.
CC {ECO:0000269|PubMed:15358670}.
CC -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC number of yeast and animal transcription factors.
CC {ECO:0000250|UniProtKB:Q8IXZ3}.
CC -!- SIMILARITY: Belongs to the Sp1 C2H2-type zinc-finger protein family.
CC {ECO:0000305}.
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DR EMBL; AY591906; AAU04515.1; -; mRNA.
DR RefSeq; NP_001185595.1; NM_001198666.1.
DR AlphaFoldDB; Q64HY5; -.
DR SMR; Q64HY5; -.
DR PaxDb; Q64HY5; -.
DR Ensembl; ENSGALT00000048259; ENSGALP00000046361; ENSGALG00000035725.
DR GeneID; 776034; -.
DR KEGG; gga:776034; -.
DR CTD; 221833; -.
DR VEuPathDB; HostDB:geneid_776034; -.
DR GeneTree; ENSGT00940000162033; -.
DR InParanoid; Q64HY5; -.
DR OMA; MYSRHPY; -.
DR OrthoDB; 664813at2759; -.
DR PhylomeDB; Q64HY5; -.
DR PRO; PR:Q64HY5; -.
DR Proteomes; UP000000539; Chromosome 2.
DR Bgee; ENSGALG00000035725; Expressed in liver.
DR ExpressionAtlas; Q64HY5; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0030326; P:embryonic limb morphogenesis; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 2.
DR SMART; SM00355; ZnF_C2H2; 3.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
PE 2: Evidence at transcript level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..480
FT /note="Transcription factor Sp8"
FT /id="PRO_0000395447"
FT ZN_FING 342..366
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 372..396
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 402..424
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 280..308
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 416..480
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 194..202
FT /note="9aaTAD"
FT /evidence="ECO:0000250|UniProtKB:Q8IXZ3"
FT COMPBIAS 8..24
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 480 AA; 47402 MW; C7692D5153FB7D17 CRC64;
MLAATCNKIG SPSPSPSALS DSASSFGKGF HPWKRSSSSS SASAGSCGAV GSGLPGFGVA
GAARNGSSAA AAAAAAAAAA LVSDSFSCGG SPGSSAFSLT SSGAAAASSP FANDYSVFQA
PGSAGGGGGG GGGGGGAAGQ EAAHQPVFIS KVHASVEGLQ GIYPRVGMAH PYESWFKPSH
PGLAAGEVGS AGASSWWDVG AGWIDVQSPN GAAALPGSLH PAAGGLQSSL HSPLGGYNSD
YSGLGHSAFG GGASSHLLSP AGQHLMDGFK PVLPGSYPDS APSPLAGAGG SMLGGGPAAP
LSASPRSSAR RYSGRATCDC PNCQEAERLG PAGASLRRKG LHSCHIPGCG KVYGKTSHLK
AHLRWHTGER PFVCNWLFCG KRFTRSDELQ RHLRTHTGEK RFACPVCNKR FMRSDHLSKH
VKTHSGPGGA GGPGGGGPGP GPGGKKGSDT DSEHSAAGSP PCHSPELLPP PEPGHRNGLE
