ID   SP8_DANRE               Reviewed;         437 AA.
AC   Q6P0J3; Q6ED30;
DT   13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=Transcription factor Sp8;
GN   Name=sp8b; Synonyms=sp8l;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RX   PubMed=15358670; DOI=10.1242/dev.01331;
RA   Kawakami Y., Rodriguez Esteban C., Matsui T., Rodriguez-Leon J., Kato S.,
RA   Izpisua Belmonte J.C.;
RT   "Sp8 and Sp9, two closely related buttonhead-like transcription factors,
RT   regulate Fgf8 expression and limb outgrowth in vertebrate embryos.";
RL   Development 131:4763-4774(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Zhao C., Meng A.;
RT   "Zebrafish Sp8 is involved in neurogenesis.";
RL   Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transcription factor which plays a key role in limb
CC       development. Positively regulates FGF8 expression in the apical
CC       ectodermal ridge (AER) and contributes to limb outgrowth in embryos.
CC       {ECO:0000269|PubMed:15358670}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- DEVELOPMENTAL STAGE: Detected in the forebrain, prospective
CC       midbrain/hindbrain boundary, tail bud, and neural keel and neural tube
CC       in the somitogenesis stages. Expressed in the pectoral fin bud, and the
CC       signal is restricted to the apical fold. {ECO:0000269|PubMed:15358670}.
CC   -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC       number of yeast and animal transcription factors.
CC       {ECO:0000250|UniProtKB:Q8IXZ3}.
CC   -!- SIMILARITY: Belongs to the Sp1 C2H2-type zinc-finger protein family.
CC       {ECO:0000305}.
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DR   EMBL; AY591904; AAU04513.1; -; mRNA.
DR   EMBL; AY457141; AAS16485.1; -; mRNA.
DR   EMBL; BC065597; AAH65597.1; -; mRNA.
DR   RefSeq; NP_991113.1; NM_205550.2.
DR   RefSeq; XP_005158016.1; XM_005157959.3.
DR   AlphaFoldDB; Q6P0J3; -.
DR   SMR; Q6P0J3; -.
DR   STRING; 7955.ENSDARP00000073657; -.
DR   PaxDb; Q6P0J3; -.
DR   Ensembl; ENSDART00000112894; ENSDARP00000101509; ENSDARG00000056666.
DR   Ensembl; ENSDART00000190590; ENSDARP00000156612; ENSDARG00000056666.
DR   GeneID; 324931; -.
DR   KEGG; dre:324931; -.
DR   CTD; 324931; -.
DR   ZFIN; ZDB-GENE-030131-3654; sp8b.
DR   eggNOG; KOG1721; Eukaryota.
DR   GeneTree; ENSGT00940000162033; -.
DR   InParanoid; Q6P0J3; -.
DR   OMA; MYSRHPY; -.
DR   PhylomeDB; Q6P0J3; -.
DR   Reactome; R-DRE-2173796; SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
DR   Reactome; R-DRE-3232118; SUMOylation of transcription factors.
DR   Reactome; R-DRE-6807505; RNA polymerase II transcribes snRNA genes.
DR   Reactome; R-DRE-9018519; Estrogen-dependent gene expression.
DR   PRO; PR:Q6P0J3; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 16.
DR   Bgee; ENSDARG00000056666; Expressed in neural plate and 28 other tissues.
DR   ExpressionAtlas; Q6P0J3; baseline.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0003690; F:double-stranded DNA binding; IDA:ZFIN.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0030326; P:embryonic limb morphogenesis; IMP:UniProtKB.
DR   GO; GO:0035118; P:embryonic pectoral fin morphogenesis; IMP:ZFIN.
DR   GO; GO:0045743; P:positive regulation of fibroblast growth factor receptor signaling pathway; IMP:ZFIN.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR036236; Znf_C2H2_sf.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   Pfam; PF00096; zf-C2H2; 2.
DR   SMART; SM00355; ZnF_C2H2; 3.
DR   SUPFAM; SSF57667; SSF57667; 2.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
PE   2: Evidence at transcript level;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat;
KW   Transcription; Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..437
FT                   /note="Transcription factor Sp8"
FT                   /id="PRO_0000395448"
FT   ZN_FING         297..321
FT                   /note="C2H2-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         327..351
FT                   /note="C2H2-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         357..379
FT                   /note="C2H2-type 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   REGION          1..24
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          232..263
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          373..437
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           154..162
FT                   /note="9aaTAD"
FT                   /evidence="ECO:0000250|UniProtKB:Q8IXZ3"
FT   COMPBIAS        8..24
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        378..396
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        420..437
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        8
FT                   /note="K -> T (in Ref. 2; AAS16485)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        53..54
FT                   /note="NG -> S (in Ref. 2; AAS16485)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   437 AA;  45699 MW;  ED4A975B1628FC81 CRC64;
     MLAATCNKIG SPSPSPSSIS DNSSSFGKGF HPWKRATASS CSLGSSLSSF TRNGGGLSDS
     FGTNTSTGSS AFSLTSGTSS NSHFGNDYSV FQTSVSNNSQ EPSHQPMFIS KVHTSVDSLQ
     SIYPRMSVAH PYESWFKSSH PGIPTGDVGT TGASAWWDVG AGWIDVQNPN GAALQTSLHS
     GGLQTSLHSP LGGYNSDYSS LSHSAFSTSA SPHLLTTGQH LMDGFKPVLS SYPDSSPSPL
     GGAGGSMLTG GPTASLAGSP RSSARRYSGR ATCDCPNCQE AERLGPAGAS LRRKGLHSCH
     IPGCGKVYGK TSHLKAHLRW HTGERPFVCN WLFCGKRFTR SDELQRHLRT HTGEKRFACP
     VCNKRFMRSD HLSKHVKTHS AGGSSGSGSG ASGGKRGSDT DSEHSVPGSP SCHSPDLLQA
     PESVPTTGMN GRASSLD