SP8_DANRE
ID SP8_DANRE Reviewed; 437 AA.
AC Q6P0J3; Q6ED30;
DT 13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Transcription factor Sp8;
GN Name=sp8b; Synonyms=sp8l;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=15358670; DOI=10.1242/dev.01331;
RA Kawakami Y., Rodriguez Esteban C., Matsui T., Rodriguez-Leon J., Kato S.,
RA Izpisua Belmonte J.C.;
RT "Sp8 and Sp9, two closely related buttonhead-like transcription factors,
RT regulate Fgf8 expression and limb outgrowth in vertebrate embryos.";
RL Development 131:4763-4774(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Zhao C., Meng A.;
RT "Zebrafish Sp8 is involved in neurogenesis.";
RL Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Transcription factor which plays a key role in limb
CC development. Positively regulates FGF8 expression in the apical
CC ectodermal ridge (AER) and contributes to limb outgrowth in embryos.
CC {ECO:0000269|PubMed:15358670}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Detected in the forebrain, prospective
CC midbrain/hindbrain boundary, tail bud, and neural keel and neural tube
CC in the somitogenesis stages. Expressed in the pectoral fin bud, and the
CC signal is restricted to the apical fold. {ECO:0000269|PubMed:15358670}.
CC -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC number of yeast and animal transcription factors.
CC {ECO:0000250|UniProtKB:Q8IXZ3}.
CC -!- SIMILARITY: Belongs to the Sp1 C2H2-type zinc-finger protein family.
CC {ECO:0000305}.
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DR EMBL; AY591904; AAU04513.1; -; mRNA.
DR EMBL; AY457141; AAS16485.1; -; mRNA.
DR EMBL; BC065597; AAH65597.1; -; mRNA.
DR RefSeq; NP_991113.1; NM_205550.2.
DR RefSeq; XP_005158016.1; XM_005157959.3.
DR AlphaFoldDB; Q6P0J3; -.
DR SMR; Q6P0J3; -.
DR STRING; 7955.ENSDARP00000073657; -.
DR PaxDb; Q6P0J3; -.
DR Ensembl; ENSDART00000112894; ENSDARP00000101509; ENSDARG00000056666.
DR Ensembl; ENSDART00000190590; ENSDARP00000156612; ENSDARG00000056666.
DR GeneID; 324931; -.
DR KEGG; dre:324931; -.
DR CTD; 324931; -.
DR ZFIN; ZDB-GENE-030131-3654; sp8b.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000162033; -.
DR InParanoid; Q6P0J3; -.
DR OMA; MYSRHPY; -.
DR PhylomeDB; Q6P0J3; -.
DR Reactome; R-DRE-2173796; SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
DR Reactome; R-DRE-3232118; SUMOylation of transcription factors.
DR Reactome; R-DRE-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-DRE-9018519; Estrogen-dependent gene expression.
DR PRO; PR:Q6P0J3; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 16.
DR Bgee; ENSDARG00000056666; Expressed in neural plate and 28 other tissues.
DR ExpressionAtlas; Q6P0J3; baseline.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0003690; F:double-stranded DNA binding; IDA:ZFIN.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0030326; P:embryonic limb morphogenesis; IMP:UniProtKB.
DR GO; GO:0035118; P:embryonic pectoral fin morphogenesis; IMP:ZFIN.
DR GO; GO:0045743; P:positive regulation of fibroblast growth factor receptor signaling pathway; IMP:ZFIN.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 2.
DR SMART; SM00355; ZnF_C2H2; 3.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
PE 2: Evidence at transcript level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..437
FT /note="Transcription factor Sp8"
FT /id="PRO_0000395448"
FT ZN_FING 297..321
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 327..351
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 357..379
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 232..263
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 373..437
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 154..162
FT /note="9aaTAD"
FT /evidence="ECO:0000250|UniProtKB:Q8IXZ3"
FT COMPBIAS 8..24
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 378..396
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 420..437
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 8
FT /note="K -> T (in Ref. 2; AAS16485)"
FT /evidence="ECO:0000305"
FT CONFLICT 53..54
FT /note="NG -> S (in Ref. 2; AAS16485)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 437 AA; 45699 MW; ED4A975B1628FC81 CRC64;
MLAATCNKIG SPSPSPSSIS DNSSSFGKGF HPWKRATASS CSLGSSLSSF TRNGGGLSDS
FGTNTSTGSS AFSLTSGTSS NSHFGNDYSV FQTSVSNNSQ EPSHQPMFIS KVHTSVDSLQ
SIYPRMSVAH PYESWFKSSH PGIPTGDVGT TGASAWWDVG AGWIDVQNPN GAALQTSLHS
GGLQTSLHSP LGGYNSDYSS LSHSAFSTSA SPHLLTTGQH LMDGFKPVLS SYPDSSPSPL
GGAGGSMLTG GPTASLAGSP RSSARRYSGR ATCDCPNCQE AERLGPAGAS LRRKGLHSCH
IPGCGKVYGK TSHLKAHLRW HTGERPFVCN WLFCGKRFTR SDELQRHLRT HTGEKRFACP
VCNKRFMRSD HLSKHVKTHS AGGSSGSGSG ASGGKRGSDT DSEHSVPGSP SCHSPDLLQA
PESVPTTGMN GRASSLD