SP8_MOUSE
ID SP8_MOUSE Reviewed; 486 AA.
AC Q8BMJ8; Q8BWA3;
DT 16-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Transcription factor Sp8;
GN Name=Sp8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=15358670; DOI=10.1242/dev.01331;
RA Kawakami Y., Rodriguez Esteban C., Matsui T., Rodriguez-Leon J., Kato S.,
RA Izpisua Belmonte J.C.;
RT "Sp8 and Sp9, two closely related buttonhead-like transcription factors,
RT regulate Fgf8 expression and limb outgrowth in vertebrate embryos.";
RL Development 131:4763-4774(2004).
CC -!- FUNCTION: Transcription factor which plays a key role in limb
CC development. Positively regulates FGF8 expression in the apical
CC ectodermal ridge (AER) and contributes to limb outgrowth in embryos.
CC {ECO:0000269|PubMed:15358670}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expression is detected in the forebrain,
CC midbrain/hindbrain boundary and neural tube. At the stage of limb bud
CC outgrowth, expressed in a scattered manner in the ventral ectoderm, and
CC is later confined to the apical ectodermal ridge (AER).
CC {ECO:0000269|PubMed:15358670}.
CC -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC number of yeast and animal transcription factors.
CC {ECO:0000250|UniProtKB:Q8IXZ3}.
CC -!- SIMILARITY: Belongs to the Sp1 C2H2-type zinc-finger protein family.
CC {ECO:0000305}.
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DR EMBL; AK030745; BAC27113.1; -; mRNA.
DR EMBL; AK053093; BAC35264.1; -; mRNA.
DR CCDS; CCDS26213.1; -.
DR RefSeq; NP_796056.2; NM_177082.4.
DR RefSeq; XP_006516046.1; XM_006515983.3.
DR RefSeq; XP_006516047.1; XM_006515984.3.
DR RefSeq; XP_006516048.1; XM_006515985.3.
DR RefSeq; XP_006516049.1; XM_006515986.3.
DR RefSeq; XP_006516050.1; XM_006515987.3.
DR RefSeq; XP_006516051.1; XM_006515988.3.
DR RefSeq; XP_011242426.1; XM_011244124.2.
DR AlphaFoldDB; Q8BMJ8; -.
DR SMR; Q8BMJ8; -.
DR STRING; 10090.ENSMUSP00000065746; -.
DR iPTMnet; Q8BMJ8; -.
DR PhosphoSitePlus; Q8BMJ8; -.
DR MaxQB; Q8BMJ8; -.
DR PaxDb; Q8BMJ8; -.
DR PRIDE; Q8BMJ8; -.
DR ProteomicsDB; 257332; -.
DR Antibodypedia; 53277; 68 antibodies from 15 providers.
DR DNASU; 320145; -.
DR Ensembl; ENSMUST00000063918; ENSMUSP00000065746; ENSMUSG00000048562.
DR GeneID; 320145; -.
DR KEGG; mmu:320145; -.
DR UCSC; uc007pig.2; mouse.
DR CTD; 221833; -.
DR MGI; MGI:2443471; Sp8.
DR VEuPathDB; HostDB:ENSMUSG00000048562; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000162033; -.
DR HOGENOM; CLU_019484_4_1_1; -.
DR InParanoid; Q8BMJ8; -.
DR OMA; MYSRHPY; -.
DR OrthoDB; 664813at2759; -.
DR PhylomeDB; Q8BMJ8; -.
DR TreeFam; TF350150; -.
DR BioGRID-ORCS; 320145; 3 hits in 74 CRISPR screens.
DR PRO; PR:Q8BMJ8; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q8BMJ8; protein.
DR Bgee; ENSMUSG00000048562; Expressed in rostral migratory stream and 64 other tissues.
DR ExpressionAtlas; Q8BMJ8; baseline and differential.
DR Genevisible; Q8BMJ8; MM.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; ISS:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0009953; P:dorsal/ventral pattern formation; IMP:MGI.
DR GO; GO:0030326; P:embryonic limb morphogenesis; IMP:UniProtKB.
DR GO; GO:0009954; P:proximal/distal pattern formation; IMP:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 2.
DR SMART; SM00355; ZnF_C2H2; 3.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
PE 2: Evidence at transcript level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..486
FT /note="Transcription factor Sp8"
FT /id="PRO_0000047153"
FT ZN_FING 356..380
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 386..410
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 416..438
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 1..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 136..157
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 294..324
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 432..486
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 208..216
FT /note="9aaTAD"
FT /evidence="ECO:0000250|UniProtKB:Q8IXZ3"
FT COMPBIAS 8..46
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 486 AA; 48417 MW; 336C36B548646883 CRC64;
MLAATCNKIG SPSPSPSSLS DSSSSFGKGF HPWKRSSSSS SGSCNVVGSS LSSFGVSGAS
RNGGSSSAAA AAAAAAAAAA ALVSDSFSCG GSPGSSAFSL TSSSAAAAAA AAAAAASSSP
FANDYSVFQA PGVSGGSGGG GGGGGGGSGA HSQDSSHQPV FISKVHTSVD GLQGIYPRVG
MAHPYESWFK PSHPGLGAAA DVGSAGASSW WDVGAGWIDV QNPNGAAALP GSLHPAAGGL
QTSLHSPLGG YNSDYSGLSH SAFSSGASSH LLSPAGQHLM DGFKPVLPGS YPDSAPSPLA
GAGSSMLSAG PAAQLGGSPR SSARRYSGRA TCDCPNCQEA ERLGPAGASL RRKGLHSCHI
PGCGKVYGKT SHLKAHLRWH TGERPFVCNW LFCGKRFTRS DELQRHLRTH TGEKRFACPV
CNKRFMRSDH LSKHVKTHSG GGGSAGSGGG KKGSDTDSEH SAAGSPPCHS PELLQPPEPG
HRNGLE
