SP8_XENLA
ID SP8_XENLA Reviewed; 500 AA.
AC Q5XGT8;
DT 13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Transcription factor Sp8;
GN Name=sp8;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Transcription factor which plays a key role in limb
CC development. Positively regulates FGF8 expression in the apical
CC ectodermal ridge (AER) and contributes to limb outgrowth in embryos (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC number of yeast and animal transcription factors.
CC {ECO:0000250|UniProtKB:Q8IXZ3}.
CC -!- SIMILARITY: Belongs to the Sp1 C2H2-type zinc-finger protein family.
CC {ECO:0000305}.
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DR EMBL; BC084343; AAH84343.1; -; mRNA.
DR RefSeq; NP_001088307.1; NM_001094838.1.
DR AlphaFoldDB; Q5XGT8; -.
DR SMR; Q5XGT8; -.
DR DNASU; 495143; -.
DR GeneID; 495143; -.
DR KEGG; xla:495143; -.
DR CTD; 495143; -.
DR Xenbase; XB-GENE-1216193; sp8.L.
DR OMA; YASAWWD; -.
DR OrthoDB; 664813at2759; -.
DR Proteomes; UP000186698; Chromosome 6L.
DR Bgee; 495143; Expressed in brain and 4 other tissues.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 2.
DR SMART; SM00355; ZnF_C2H2; 3.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
PE 2: Evidence at transcript level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..500
FT /note="Transcription factor Sp8"
FT /id="PRO_0000395449"
FT ZN_FING 369..393
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 399..423
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 429..451
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 1..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 304..337
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 445..500
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 210..218
FT /note="9aaTAD"
FT /evidence="ECO:0000250|UniProtKB:Q8IXZ3"
FT COMPBIAS 8..25
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 32..46
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 451..469
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 500 AA; 49231 MW; 2BF930EF55C8959C CRC64;
MLAATCNKIG SPSPSPSSLS DTSGSFGKGF HPWKRSSSTS SSCTLASGSL SGFSVGGSSR
SANGSSSSSA AAAAAAAAAA AAAAALVSDS FSCGGSPGSS AFSLTSGGSG GSGGGGSSGS
MSAASPFANE YSVFPVSGGS QEAAAAAAAA AAAAAAAASH HQPVFLSKVH ASVDGLQSIY
PRVGMAHPYE SWFKPSHPGE VSAAAAGGAS SWWEVGAGWI DVQSPGAGAA LHPGALQGSL
HSPLGGYGTD YSAAGLGHFS TGGGGGVGSG GGAAGTGSGG GGGGSHLLSP GGQHLMDGFK
PVIGPGSYPD PSSSPLSAGS MLAPTGPLGG SPRSSARRYS GRATCDCPNC QEAERLGPAG
ASLRRKGLHS CHIPGCGKVY GKTSHLKAHL RWHTGERPFV CNWLFCGKRF TRSDELQRHL
RTHTGEKRFA CPVCNKRFMR SDHLSKHVKT HSVGGGSTGS TGPGSGSKKG SDTDSEHSPS
TSPTCHSPDL LHPPDRNGLE