ID   SP8_XENLA               Reviewed;         500 AA.
AC   Q5XGT8;
DT   13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Transcription factor Sp8;
GN   Name=sp8;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Eye;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transcription factor which plays a key role in limb
CC       development. Positively regulates FGF8 expression in the apical
CC       ectodermal ridge (AER) and contributes to limb outgrowth in embryos (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC       number of yeast and animal transcription factors.
CC       {ECO:0000250|UniProtKB:Q8IXZ3}.
CC   -!- SIMILARITY: Belongs to the Sp1 C2H2-type zinc-finger protein family.
CC       {ECO:0000305}.
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DR   EMBL; BC084343; AAH84343.1; -; mRNA.
DR   RefSeq; NP_001088307.1; NM_001094838.1.
DR   AlphaFoldDB; Q5XGT8; -.
DR   SMR; Q5XGT8; -.
DR   DNASU; 495143; -.
DR   GeneID; 495143; -.
DR   KEGG; xla:495143; -.
DR   CTD; 495143; -.
DR   Xenbase; XB-GENE-1216193; sp8.L.
DR   OMA; YASAWWD; -.
DR   OrthoDB; 664813at2759; -.
DR   Proteomes; UP000186698; Chromosome 6L.
DR   Bgee; 495143; Expressed in brain and 4 other tissues.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   InterPro; IPR036236; Znf_C2H2_sf.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   Pfam; PF00096; zf-C2H2; 2.
DR   SMART; SM00355; ZnF_C2H2; 3.
DR   SUPFAM; SSF57667; SSF57667; 2.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
PE   2: Evidence at transcript level;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat;
KW   Transcription; Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..500
FT                   /note="Transcription factor Sp8"
FT                   /id="PRO_0000395449"
FT   ZN_FING         369..393
FT                   /note="C2H2-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         399..423
FT                   /note="C2H2-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         429..451
FT                   /note="C2H2-type 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   REGION          1..46
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          304..337
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          445..500
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           210..218
FT                   /note="9aaTAD"
FT                   /evidence="ECO:0000250|UniProtKB:Q8IXZ3"
FT   COMPBIAS        8..25
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        32..46
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        451..469
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   500 AA;  49231 MW;  2BF930EF55C8959C CRC64;
     MLAATCNKIG SPSPSPSSLS DTSGSFGKGF HPWKRSSSTS SSCTLASGSL SGFSVGGSSR
     SANGSSSSSA AAAAAAAAAA AAAAALVSDS FSCGGSPGSS AFSLTSGGSG GSGGGGSSGS
     MSAASPFANE YSVFPVSGGS QEAAAAAAAA AAAAAAAASH HQPVFLSKVH ASVDGLQSIY
     PRVGMAHPYE SWFKPSHPGE VSAAAAGGAS SWWEVGAGWI DVQSPGAGAA LHPGALQGSL
     HSPLGGYGTD YSAAGLGHFS TGGGGGVGSG GGAAGTGSGG GGGGSHLLSP GGQHLMDGFK
     PVIGPGSYPD PSSSPLSAGS MLAPTGPLGG SPRSSARRYS GRATCDCPNC QEAERLGPAG
     ASLRRKGLHS CHIPGCGKVY GKTSHLKAHL RWHTGERPFV CNWLFCGKRF TRSDELQRHL
     RTHTGEKRFA CPVCNKRFMR SDHLSKHVKT HSVGGGSTGS TGPGSGSKKG SDTDSEHSPS
     TSPTCHSPDL LHPPDRNGLE