SP9_DANRE
ID SP9_DANRE Reviewed; 439 AA.
AC Q6NW96; Q64HY6;
DT 13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Transcription factor Sp9;
GN Name=sp9;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=15358670; DOI=10.1242/dev.01331;
RA Kawakami Y., Rodriguez Esteban C., Matsui T., Rodriguez-Leon J., Kato S.,
RA Izpisua Belmonte J.C.;
RT "Sp8 and Sp9, two closely related buttonhead-like transcription factors,
RT regulate Fgf8 expression and limb outgrowth in vertebrate embryos.";
RL Development 131:4763-4774(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA van den Akker W.M.R., Mansouri A., Durston A.J.;
RT "Sp9, a new member of the Sp family of zinc-finger transcription factors,
RT is dynamically expressed in brain, tail and pectoral fins during zebrafish
RT development.";
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Transcription factor which plays a key role in limb
CC development. Positively regulates FGF8 expression in the apical
CC ectodermal ridge (AER) and contributes to limb outgrowth in embryos.
CC {ECO:0000269|PubMed:15358670}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6NW96-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6NW96-2; Sequence=VSP_039468;
CC -!- DEVELOPMENTAL STAGE: Expressed in the forebrain, and in the prospective
CC midbrain/hindbrain boundary during early somitogenesis. As development
CC proceeds, also detected in the hindbrain, as well as in the apical fold
CC of the developing pectoral fin, and this expression extends proximally.
CC {ECO:0000269|PubMed:15358670}.
CC -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC number of yeast and animal transcription factors. In SP9, the motif is
CC inactive. {ECO:0000250|UniProtKB:P0CG40}.
CC -!- SIMILARITY: Belongs to the Sp1 C2H2-type zinc-finger protein family.
CC {ECO:0000305}.
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DR EMBL; AY591905; AAU04514.1; -; mRNA.
DR EMBL; AY731230; AAV34441.1; -; mRNA.
DR EMBL; BC067673; AAH67673.1; -; mRNA.
DR RefSeq; NP_998125.2; NM_212960.2. [Q6NW96-2]
DR RefSeq; XP_005167471.1; XM_005167414.3. [Q6NW96-1]
DR AlphaFoldDB; Q6NW96; -.
DR SMR; Q6NW96; -.
DR STRING; 7955.ENSDARP00000110885; -.
DR PaxDb; Q6NW96; -.
DR Ensembl; ENSDART00000164276; ENSDARP00000141658; ENSDARG00000099374. [Q6NW96-1]
DR Ensembl; ENSDART00000188306; ENSDARP00000145264; ENSDARG00000099374. [Q6NW96-2]
DR GeneID; 405896; -.
DR KEGG; dre:405896; -.
DR CTD; 100131390; -.
DR ZFIN; ZDB-GENE-040426-2313; sp9.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000162304; -.
DR HOGENOM; CLU_019484_4_1_1; -.
DR InParanoid; Q6NW96; -.
DR OMA; NGASTWW; -.
DR OrthoDB; 664813at2759; -.
DR PhylomeDB; Q6NW96; -.
DR TreeFam; TF350150; -.
DR PRO; PR:Q6NW96; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 9.
DR Bgee; ENSDARG00000099374; Expressed in larva and 14 other tissues.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0030326; P:embryonic limb morphogenesis; IMP:UniProtKB.
DR GO; GO:0035118; P:embryonic pectoral fin morphogenesis; IMP:ZFIN.
DR GO; GO:0045743; P:positive regulation of fibroblast growth factor receptor signaling pathway; IMP:ZFIN.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 3.
DR SMART; SM00355; ZnF_C2H2; 3.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..439
FT /note="Transcription factor Sp9"
FT /id="PRO_0000395452"
FT ZN_FING 308..332
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 338..362
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 368..390
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 384..439
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 166..174
FT /note="9aaTAD; inactive"
FT /evidence="ECO:0000250|UniProtKB:P0CG40"
FT COMPBIAS 400..414
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..7
FT /note="MATSILG -> MFYSLVKSTNFSSTQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15358670"
FT /id="VSP_039468"
SQ SEQUENCE 439 AA; 46772 MW; 32C5DE41A663823F CRC64;
MATSILGEEP RFGTTPLAML AATCNKIGNT SPLTTLPDSS AFSKGGFHPW KRSSSSCNLG
SSLPGFTVAT SRSTSGLTTT TGAGNSAFCL ASTSPTSSAF SAEYSSLFSN TTSVSSQDSG
QSAFISKVHS SADTLYPRVG MAHPYESWYK SGFHSTISGD VANGASTWWD VHTNPGSWLE
VQNPAGTLQS SLHSGTPQAI HSQLSGYNPD FSSLTHSAFS STGISPTASH LLSTSQHLLT
QEGFKTVIPT YTDASATNAM ISGASSGIGT SSRSSRRYSG RATCDCPNCQ EAERLGPAGA
SLRRKGLHSC HIPGCGKVYG KTSHLKAHLR WHTGERPFVC NWLFCGKRFT RSDELQRHLR
THTGEKRFAC PVCNKRFMRS DHLSKHIKTH TAGGGGKKGS DSDTDTSNLE TPRSESPELI
LEGVNPRVTV KDQSPHHES
