SPA24_HUMAN
ID SPA24_HUMAN Reviewed; 205 AA.
AC Q86W54;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Spermatogenesis-associated protein 24;
DE AltName: Full=Testis protein T6441 homolog;
GN Name=SPATA24;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain, and Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15372022; DOI=10.1038/nature02919;
RA Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S.,
RA Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M.,
RA She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.,
RA Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M.,
RA Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M.,
RA Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T.,
RA Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A.,
RA Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R.,
RA Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L.,
RA Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N.,
RA Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J.,
RA Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A.,
RA Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.;
RT "The DNA sequence and comparative analysis of human chromosome 5.";
RL Nature 431:268-274(2004).
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=19515240; DOI=10.1186/1471-2091-10-16;
RA Pitulescu M.E., Teichmann M., Luo L., Kessel M.;
RT "TIPT2 and geminin interact with basal transcription factors to synergize
RT in transcriptional regulation.";
RL BMC Biochem. 10:16-16(2009).
CC -!- FUNCTION: Binds DNA with high affinity but does not bind to TATA boxes.
CC Synergises with GMNN and TBP in activation of TATA box-containing
CC promoters and with GMNN and TBPL1 in activation of the NF1 TATA-less
CC promoter. May play a role in cytoplasm movement and removal during
CC spermiogenesis (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. Interacts with CBX3, CBX5, GMNN, GTF2B, TBPL1 and
CC the polycomb proteins PHCF2, RNF2 and SCMH1 but not with CBX1 or PCGF2
CC (By similarity). {ECO:0000250}.
CC -!- INTERACTION:
CC Q86W54; Q13137: CALCOCO2; NbExp=3; IntAct=EBI-3916986, EBI-739580;
CC Q86W54; Q9P209: CEP72; NbExp=4; IntAct=EBI-3916986, EBI-739498;
CC Q86W54; Q15323: KRT31; NbExp=3; IntAct=EBI-3916986, EBI-948001;
CC Q86W54; Q9BYR5: KRTAP4-2; NbExp=3; IntAct=EBI-3916986, EBI-10172511;
CC Q86W54; Q9BRK4: LZTS2; NbExp=3; IntAct=EBI-3916986, EBI-741037;
CC Q86W54; Q9UJV3-2: MID2; NbExp=3; IntAct=EBI-3916986, EBI-10172526;
CC Q86W54-2; Q9NSA3: CTNNBIP1; NbExp=3; IntAct=EBI-12041693, EBI-747082;
CC Q86W54-2; A8MQ03: CYSRT1; NbExp=3; IntAct=EBI-12041693, EBI-3867333;
CC Q86W54-2; G5E9A7: DMWD; NbExp=3; IntAct=EBI-12041693, EBI-10976677;
CC Q86W54-2; O95967: EFEMP2; NbExp=3; IntAct=EBI-12041693, EBI-743414;
CC Q86W54-2; P22607: FGFR3; NbExp=3; IntAct=EBI-12041693, EBI-348399;
CC Q86W54-2; P06396: GSN; NbExp=3; IntAct=EBI-12041693, EBI-351506;
CC Q86W54-2; P01112: HRAS; NbExp=3; IntAct=EBI-12041693, EBI-350145;
CC Q86W54-2; P60409: KRTAP10-7; NbExp=3; IntAct=EBI-12041693, EBI-10172290;
CC Q86W54-2; P60410: KRTAP10-8; NbExp=3; IntAct=EBI-12041693, EBI-10171774;
CC Q86W54-2; P31153: MAT2A; NbExp=3; IntAct=EBI-12041693, EBI-1050743;
CC Q86W54-2; Q99750: MDFI; NbExp=3; IntAct=EBI-12041693, EBI-724076;
CC Q86W54-2; P0DPK4: NOTCH2NLC; NbExp=3; IntAct=EBI-12041693, EBI-22310682;
CC Q86W54-2; O43482: OIP5; NbExp=3; IntAct=EBI-12041693, EBI-536879;
CC Q86W54-2; Q8N2W9: PIAS4; NbExp=3; IntAct=EBI-12041693, EBI-473160;
CC Q86W54-2; Q9H8W4: PLEKHF2; NbExp=3; IntAct=EBI-12041693, EBI-742388;
CC Q86W54-2; Q7Z699: SPRED1; NbExp=3; IntAct=EBI-12041693, EBI-5235340;
CC Q86W54-2; Q86WV8: TSC1; NbExp=3; IntAct=EBI-12041693, EBI-12806590;
CC Q86W54-2; Q9UMX0: UBQLN1; NbExp=3; IntAct=EBI-12041693, EBI-741480;
CC Q86W54-2; Q9Y649; NbExp=3; IntAct=EBI-12041693, EBI-25900580;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19515240}. Nucleus,
CC nucleolus {ECO:0000269|PubMed:19515240}. Nucleus, nucleoplasm
CC {ECO:0000269|PubMed:19515240}. Note=Associated with chromatin.
CC {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q86W54-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q86W54-2; Sequence=VSP_032867;
CC -!- SIMILARITY: Belongs to the SPATA24 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BC040037; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BC050563; AAH50563.1; -; mRNA.
DR EMBL; AC135457; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS47274.1; -. [Q86W54-1]
DR RefSeq; NP_919272.1; NM_194296.1. [Q86W54-1]
DR AlphaFoldDB; Q86W54; -.
DR SMR; Q86W54; -.
DR BioGRID; 128415; 52.
DR IntAct; Q86W54; 55.
DR MINT; Q86W54; -.
DR STRING; 9606.ENSP00000414920; -.
DR GlyGen; Q86W54; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q86W54; -.
DR PhosphoSitePlus; Q86W54; -.
DR BioMuta; SPATA24; -.
DR DMDM; 182701380; -.
DR EPD; Q86W54; -.
DR MassIVE; Q86W54; -.
DR PaxDb; Q86W54; -.
DR PeptideAtlas; Q86W54; -.
DR PRIDE; Q86W54; -.
DR ProteomicsDB; 70119; -. [Q86W54-1]
DR ProteomicsDB; 70120; -. [Q86W54-2]
DR Antibodypedia; 45375; 27 antibodies from 11 providers.
DR DNASU; 202051; -.
DR Ensembl; ENST00000450845.7; ENSP00000414920.2; ENSG00000170469.11. [Q86W54-1]
DR Ensembl; ENST00000451821.6; ENSP00000400524.2; ENSG00000170469.11. [Q86W54-2]
DR GeneID; 202051; -.
DR KEGG; hsa:202051; -.
DR MANE-Select; ENST00000450845.7; ENSP00000414920.2; NM_194296.2; NP_919272.1.
DR UCSC; uc003lel.5; human. [Q86W54-1]
DR CTD; 202051; -.
DR GeneCards; SPATA24; -.
DR HGNC; HGNC:27322; SPATA24.
DR HPA; ENSG00000170469; Tissue enriched (testis).
DR neXtProt; NX_Q86W54; -.
DR OpenTargets; ENSG00000170469; -.
DR PharmGKB; PA165660551; -.
DR VEuPathDB; HostDB:ENSG00000170469; -.
DR eggNOG; ENOG502S3HF; Eukaryota.
DR GeneTree; ENSGT00390000007817; -.
DR HOGENOM; CLU_125524_0_0_1; -.
DR InParanoid; Q86W54; -.
DR OMA; HITKQED; -.
DR OrthoDB; 1204041at2759; -.
DR PhylomeDB; Q86W54; -.
DR TreeFam; TF338733; -.
DR PathwayCommons; Q86W54; -.
DR SignaLink; Q86W54; -.
DR BioGRID-ORCS; 202051; 10 hits in 1044 CRISPR screens.
DR GenomeRNAi; 202051; -.
DR Pharos; Q86W54; Tdark.
DR PRO; PR:Q86W54; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; Q86W54; protein.
DR Bgee; ENSG00000170469; Expressed in gingival epithelium and 139 other tissues.
DR ExpressionAtlas; Q86W54; baseline and differential.
DR Genevisible; Q86W54; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0001673; C:male germ cell nucleus; IEA:Ensembl.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IEA:Ensembl.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR InterPro; IPR029176; SPATA24.
DR PANTHER; PTHR35155; PTHR35155; 1.
DR Pfam; PF15175; SPATA24; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm; Developmental protein;
KW Differentiation; DNA-binding; Nucleus; Reference proteome; Spermatogenesis;
KW Transcription; Transcription regulation.
FT CHAIN 1..205
FT /note="Spermatogenesis-associated protein 24"
FT /id="PRO_0000328979"
FT REGION 138..185
FT /note="Required for interaction with CBX5 and TBPL1"
FT /evidence="ECO:0000250"
FT REGION 180..205
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 17..166
FT /evidence="ECO:0000255"
FT COMPBIAS 187..205
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 129..205
FT /note="EIESHIIKQEDILNGKENEIKELQQVISQQKQIFRNHMSDFRIQKQQESYMA
FT QVLDQKHKKASGTRQARSHQHPREK -> GRREARALHHGYINGLFPNHPVPVSKAGFH
FT SNTCPQLRIQAGPGGPHLRKGLKGPGRPRK (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_032867"
SQ SEQUENCE 205 AA; 23587 MW; 039AD7AAB94C69E3 CRC64;
MATPLGWSKA GSGSVCLALD QLRDVIESQE ELIHQLRNVM VLQDENFVSK EEFQAVEKKL
VEEKAAHAKT KVLLAKEEEK LQFALGEVEV LSKQLEKEKL AFEKALSSVK SKVLQESSKK
DQLITKCNEI ESHIIKQEDI LNGKENEIKE LQQVISQQKQ IFRNHMSDFR IQKQQESYMA
QVLDQKHKKA SGTRQARSHQ HPREK
