SPA24_RAT
ID SPA24_RAT Reviewed; 205 AA.
AC Q4PJT6; D3ZAG4;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 13-JUL-2010, sequence version 3.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Spermatogenesis-associated protein 24;
DE AltName: Full=Testis protein T6441;
GN Name=Spata24;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=Sprague-Dawley; TISSUE=Testis;
RX PubMed=16146721; DOI=10.2741/1787;
RA Song X.-X., Li Y.-C., Shi Y.Q., Hu X.Q., Hu Z.Y., Han C.S., Liu Y.-X.;
RT "Cloning and characterization of a novel spermiogenesis-related gene,
RT T6441, in rat testis.";
RL Front. Biosci. 11:143-150(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=19515240; DOI=10.1186/1471-2091-10-16;
RA Pitulescu M.E., Teichmann M., Luo L., Kessel M.;
RT "TIPT2 and geminin interact with basal transcription factors to synergize
RT in transcriptional regulation.";
RL BMC Biochem. 10:16-16(2009).
CC -!- FUNCTION: Binds DNA with high affinity but does not bind to TATA boxes.
CC Synergises with GMNN and TBP in activation of TATA box-containing
CC promoters and with GMNN and TBPL1 in activation of the NF1 TATA-less
CC promoter (By similarity). May play a role in cytoplasm movement and
CC removal during spermiogenesis. {ECO:0000250,
CC ECO:0000269|PubMed:16146721}.
CC -!- SUBUNIT: Homodimer. Interacts with CBX3, CBX5, GMNN, GTF2B, TBPL1 and
CC the polycomb proteins PHCF2, RNF2 and SCMH1 but not with CBX1 or PCGF2
CC (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus, nucleolus. Nucleus,
CC nucleoplasm. Note=Associated with chromatin. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q4PJT6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q4PJT6-2; Sequence=VSP_039460;
CC -!- TISSUE SPECIFICITY: Highly expressed in the testis and is mainly
CC localized in the spermatids. Also expressed in the lung, heart, spleen
CC and epididymis. {ECO:0000269|PubMed:16146721}.
CC -!- DEVELOPMENTAL STAGE: Expression is low before postnatal stage on day
CC 20, and increases significantly between days 20 to 28.
CC {ECO:0000269|PubMed:16146721}.
CC -!- SIMILARITY: Belongs to the SPATA24 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAY83365.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; DQ080016; AAY83365.1; ALT_INIT; mRNA.
DR EMBL; CH473974; EDL76272.1; -; Genomic_DNA.
DR RefSeq; NP_001020807.2; NM_001025636.2.
DR RefSeq; XP_006254607.1; XM_006254545.3. [Q4PJT6-1]
DR AlphaFoldDB; Q4PJT6; -.
DR SMR; Q4PJT6; -.
DR STRING; 10116.ENSRNOP00000043703; -.
DR PaxDb; Q4PJT6; -.
DR Ensembl; ENSRNOT00000040091; ENSRNOP00000043703; ENSRNOG00000019976. [Q4PJT6-1]
DR GeneID; 291676; -.
DR KEGG; rno:291676; -.
DR UCSC; RGD:1311742; rat. [Q4PJT6-1]
DR CTD; 202051; -.
DR RGD; 1311742; Spata24.
DR eggNOG; ENOG502S3HF; Eukaryota.
DR GeneTree; ENSGT00390000007817; -.
DR HOGENOM; CLU_115899_0_0_1; -.
DR InParanoid; Q4PJT6; -.
DR OMA; HITKQED; -.
DR OrthoDB; 1521659at2759; -.
DR PhylomeDB; Q4PJT6; -.
DR TreeFam; TF338733; -.
DR PRO; PR:Q4PJT6; -.
DR Proteomes; UP000002494; Chromosome 18.
DR Proteomes; UP000234681; Chromosome 18.
DR Bgee; ENSRNOG00000019976; Expressed in testis and 19 other tissues.
DR Genevisible; Q4PJT6; RN.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0001673; C:male germ cell nucleus; IEA:Ensembl.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISO:RGD.
DR GO; GO:0003677; F:DNA binding; ISO:RGD.
DR GO; GO:0042802; F:identical protein binding; ISO:RGD.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR InterPro; IPR029176; SPATA24.
DR PANTHER; PTHR35155; PTHR35155; 1.
DR Pfam; PF15175; SPATA24; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Cytoplasm; Developmental protein;
KW Differentiation; DNA-binding; Nucleus; Reference proteome; Spermatogenesis;
KW Transcription; Transcription regulation.
FT CHAIN 1..205
FT /note="Spermatogenesis-associated protein 24"
FT /id="PRO_0000328982"
FT REGION 138..185
FT /note="Required for interaction with CBX5 and TBPL1"
FT /evidence="ECO:0000250"
FT REGION 182..205
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 17..166
FT /evidence="ECO:0000255"
FT VAR_SEQ 129..205
FT /note="EIESHIIKQEDILNGKENEIKELQQVISQQRQSFRNHMSDFRIQKQQETYMA
FT QVLDQKHKKTSGTRRARSRQCSREK -> GRREARAAPQVNIHGLFSNSPMMVPKAGLH
FT PNFCPRLRIQASPGGSHLRKCLQGPGRPRK (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16146721"
FT /id="VSP_039460"
SQ SEQUENCE 205 AA; 23718 MW; 44F3BC2F560A487C CRC64;
MATPLGWSQG GSGSVCLAFD QLRDVIESQE ELIHQLRNVM VLQDENFVSK EEFQEVEKKL
VDEKAAHAKT KALLAKEEEK LQFALGEVEV LSKQLEKEKM AFEKALSSVK SRVLQESSKK
DQLITKCNEI ESHIIKQEDI LNGKENEIKE LQQVISQQRQ SFRNHMSDFR IQKQQETYMA
QVLDQKHKKT SGTRRARSRQ CSREK
