SPAB_BACIU
ID SPAB_BACIU Reviewed; 1030 AA.
AC P39774; P33114; P36360;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 25-MAY-2022, entry version 67.
DE RecName: Full=Subtilin biosynthesis protein SpaB;
GN Name=spaB; Synonyms=spaD, spaE;
OS Bacillus subtilis.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=1423;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 6633 / PCI 219 / NRS 231;
RX PubMed=8117069; DOI=10.1128/aem.60.1.1-11.1994;
RA Gutowski-Eckel Z., Klein C., Siegers K., Bohm K., Hammelmann M.,
RA Entian K.-D.;
RT "Growth phase-dependent regulation and membrane localization of SpaB, a
RT protein involved in biosynthesis of the lantibiotic subtilin.";
RL Appl. Environ. Microbiol. 60:1-11(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 6633 / PCI 219 / NRS 231;
RX PubMed=8085823; DOI=10.1128/aem.60.8.2793-2801.1994;
RA Klein C., Entian K.-D.;
RT "Genes involved in self-protection against the lantibiotic subtilin
RT produced by Bacillus subtilis ATCC 6633.";
RL Appl. Environ. Microbiol. 60:2793-2801(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 6633 / PCI 219 / NRS 231;
RX PubMed=1400221; DOI=10.1128/jb.174.20.6699-6702.1992;
RA Chung Y.J., Hansen J.N.;
RT "Determination of the sequence of spaE and identification of a promoter in
RT the subtilin (spa) operon in Bacillus subtilis.";
RL J. Bacteriol. 174:6699-6702(1992).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 641-1030.
RC STRAIN=ATCC 6633 / PCI 219 / NRS 231;
RX PubMed=1539969; DOI=10.1128/aem.58.1.132-142.1992;
RA Klein C., Kaletta C., Schnell N., Entian K.-D.;
RT "Analysis of genes involved in biosynthesis of the lantibiotic subtilin.";
RL Appl. Environ. Microbiol. 58:132-142(1992).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 852-1030.
RC STRAIN=ATCC 6633 / PCI 219 / NRS 231;
RX PubMed=1735728; DOI=10.1128/jb.174.4.1417-1422.1992;
RA Chung Y.J., Steen M.T., Hansen J.N.;
RT "The subtilin gene of Bacillus subtilis ATCC 6633 is encoded in an operon
RT that contains a homolog of the hemolysin B transport protein.";
RL J. Bacteriol. 174:1417-1422(1992).
CC -!- FUNCTION: Involved in the post-translational modification of the
CC lantibiotic subtilin.
CC -!- SUBCELLULAR LOCATION: Cell membrane. Note=Possibly associated with, and
CC anchored to, the cytoplasmic side of the membrane.
CC -!- SIMILARITY: To S.epidermidis EpiB and L.lactis NisB. {ECO:0000305}.
CC -!- CAUTION: Was originally proposed to code for two separate adjacent
CC ORFs, SpaE and SpaD. {ECO:0000305|PubMed:1400221}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA22769.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAA22774.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAA22775.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; L24075; AAA22779.1; -; Genomic_DNA.
DR EMBL; U09819; AAB91586.1; -; Genomic_DNA.
DR EMBL; M99263; AAA22774.1; ALT_FRAME; Genomic_DNA.
DR EMBL; M99263; AAA22775.1; ALT_FRAME; Genomic_DNA.
DR EMBL; M86869; AAA22837.1; -; Genomic_DNA.
DR EMBL; M83944; AAA22769.1; ALT_INIT; Genomic_DNA.
DR EMBL; U38418; AAB01536.1; -; Genomic_DNA.
DR PIR; I39987; I39987.
DR AlphaFoldDB; P39774; -.
DR SMR; P39774; -.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR006827; Lant_deHydtase_N.
DR InterPro; IPR023809; Thiopep_bacteriocin_synth_dom.
DR Pfam; PF14028; Lant_dehydr_C; 1.
DR Pfam; PF04738; Lant_dehydr_N; 1.
DR TIGRFAMs; TIGR03891; thiopep_ocin; 1.
PE 4: Predicted;
KW Cell membrane; Membrane; Transport.
FT CHAIN 1..1030
FT /note="Subtilin biosynthesis protein SpaB"
FT /id="PRO_0000072091"
FT CONFLICT 126
FT /note="L -> F (in Ref. 3; AAA22774)"
FT /evidence="ECO:0000305"
FT CONFLICT 261
FT /note="D -> N (in Ref. 3; AAA22774)"
FT /evidence="ECO:0000305"
FT CONFLICT 304..305
FT /note="LA -> AG (in Ref. 3; AAA22774)"
FT /evidence="ECO:0000305"
FT CONFLICT 344
FT /note="H -> K (in Ref. 3; AAA22774)"
FT /evidence="ECO:0000305"
FT CONFLICT 553
FT /note="N -> I (in Ref. 3; AAA22774)"
FT /evidence="ECO:0000305"
FT CONFLICT 584
FT /note="F -> L (in Ref. 3; AAA22774)"
FT /evidence="ECO:0000305"
FT CONFLICT 647
FT /note="P -> A (in Ref. 3; AAA22774)"
FT /evidence="ECO:0000305"
FT CONFLICT 730
FT /note="E -> V (in Ref. 3; AAA22774)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1030 AA; 120529 MW; 89F3B147E02A9DF8 CRC64;
MKSLYTPTDY YMIRVPLVHQ DLKNENSQDI DQLLHDLCND SLFREQILVS SRTLYETIHT
FLQAPDKLKG KKKRNFQQAI LKYATRRATR TTPFGLFSSV GIGSFSDKNH LSFNQHSFYK
KARVDLEWLY QLIRKLENEY TDRLSFTLNS ACYIKGDRAY LLYSTDGKSE EVSVRATSVF
YLINELCGES AAYQDIIRCL IDNYPNTPIN KINQYVADLI DKEFLISNLR PPMTVSDQFQ
YLIDQAESRH IPNELIQACK DIQYQIDAYN RITIGEGEHQ YLNLIETMNK LIKASSPLQV
DAGLADSSIQ LDNETSLAIS ELASMFTYMA SPSANTLDHL EKYHNVFLER YGYEREVPLL
EMLCSSTGIG APATYTNPAN EFFEETSFGE QFSPEMKQFF MRKYFESVRK KAPIQLDDET
FHRICNSEIA DEEIPLSFEL NFFVKLRNGR VKLYLGPNVG STRAGKTFGR FSHMSDSISE
IIKTLHNKEK ELTECNTKVC ELSIVPNQTR SGNVTRNVSY REKEMSLFTN SALHLNDSVK
AEDILIGINK DHNFYARHKT TGEILSFESN HMFNPLLMTN AVRFLLEISR DGKRKWNDFP
WFSIYSDFKY IPEIKYKEIT LSCEQWLIYK NDLSMHSNAS LEEIKSPFFE FHRTYELPQT
FYIVNADNRL LIDIENDCTL DVFFWELKKT NHNQPLQLVA VEHDADALMD RNQNDYSGEI
VVPLLRKQPE KPLYLPVLNA IEGSGSDRIK MPFEDWLFIK LYCKQTREEE LIAFEIADFY
NQISDQYPVR HFFMRYRDPK PHIRLRFNGK AEVLYSLFPQ LLNWLKSLRE KGLVSESVIT
QYEREIERYG GLSLMEAAEQ LFCEDSKVVE MMIRMHRMKD ITISKEIAGM VSVIQFLEQF
ELTFEEQLTF LERNSLQNEY RTEFKKDREM YIEICNSDRD WDNLKKTSDG GMLYETLKTR
KMAAAHYAFL IKKAFDNKDE VYSRIGSIIH LHCNRLFGTD RELENKILTL CRHSLYAQRY
QKMNGSLAWK
