SPAC7_MOUSE
ID SPAC7_MOUSE Reviewed; 182 AA.
AC Q9D2S4;
DT 23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Sperm acrosome-associated protein 7 {ECO:0000312|MGI:MGI:1925884};
DE Flags: Precursor;
GN Name=Spaca7 {ECO:0000312|MGI:MGI:1925884};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=22495889; DOI=10.1530/rep-11-0270;
RA Korfanty J., Toma A., Wojtas A., Rusin A., Vydra N., Widlak W.;
RT "Identification of a new mouse sperm acrosome-associated protein.";
RL Reproduction 143:749-757(2012).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=24307706; DOI=10.1095/biolreprod.113.111831;
RA Nguyen E.B., Westmuckett A.D., Moore K.L.;
RT "SPACA7 is a novel male germ cell-specific protein localized to the sperm
RT acrosome that is involved in fertilization in mice.";
RL Biol. Reprod. 90:16-16(2014).
CC -!- FUNCTION: Involved in fertilization. Seems not to play a direct role in
CC sperm-egg binding or gamete fusion. {ECO:0000269|PubMed:24307706}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22495889}.
CC Cytoplasmic vesicle, secretory vesicle, acrosome lumen
CC {ECO:0000269|PubMed:22495889, ECO:0000269|PubMed:24307706}.
CC Note=Localized in perinuclear pro-acrosomal granules in round
CC spermatides (PubMed:24307706). Localized between the inner and outer
CC acrosomal membranes (matrix or lumen) in spermatozoa (PubMed:24307706).
CC Secreted during acrosome exocytosis (PubMed:24307706).
CC {ECO:0000269|PubMed:24307706}.
CC -!- TISSUE SPECIFICITY: Testis-specific (PubMed:22495889, PubMed:24307706).
CC Expressed in zygotene and pachytene spermatocytes, round spermatids,
CC elongating spermatids and spermatozoa (at protein level)
CC (PubMed:22495889, PubMed:24307706). Testis-specific (PubMed:22495889).
CC {ECO:0000269|PubMed:22495889, ECO:0000269|PubMed:24307706}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK018926; BAB31486.1; -; mRNA.
DR CCDS; CCDS40222.1; -.
DR RefSeq; NP_077241.1; NM_024279.2.
DR AlphaFoldDB; Q9D2S4; -.
DR STRING; 10090.ENSMUSP00000010579; -.
DR GlyGen; Q9D2S4; 1 site.
DR PhosphoSitePlus; Q9D2S4; -.
DR PaxDb; Q9D2S4; -.
DR PRIDE; Q9D2S4; -.
DR ProteomicsDB; 257334; -.
DR Antibodypedia; 42580; 61 antibodies from 13 providers.
DR DNASU; 78634; -.
DR Ensembl; ENSMUST00000010579; ENSMUSP00000010579; ENSMUSG00000010435.
DR GeneID; 78634; -.
DR KEGG; mmu:78634; -.
DR UCSC; uc009kwc.1; mouse.
DR CTD; 122258; -.
DR MGI; MGI:1925884; Spaca7.
DR VEuPathDB; HostDB:ENSMUSG00000010435; -.
DR eggNOG; ENOG502TF3C; Eukaryota.
DR GeneTree; ENSGT00390000018090; -.
DR HOGENOM; CLU_120537_0_0_1; -.
DR InParanoid; Q9D2S4; -.
DR OMA; MAVNQGE; -.
DR OrthoDB; 1489307at2759; -.
DR PhylomeDB; Q9D2S4; -.
DR TreeFam; TF338340; -.
DR BioGRID-ORCS; 78634; 2 hits in 72 CRISPR screens.
DR PRO; PR:Q9D2S4; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q9D2S4; protein.
DR Bgee; ENSMUSG00000010435; Expressed in seminiferous tubule of testis and 8 other tissues.
DR ExpressionAtlas; Q9D2S4; baseline and differential.
DR Genevisible; Q9D2S4; MM.
DR GO; GO:0043160; C:acrosomal lumen; IEA:UniProtKB-SubCell.
DR GO; GO:0001669; C:acrosomal vesicle; IDA:MGI.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0007155; P:cell adhesion; IMP:MGI.
DR GO; GO:0007162; P:negative regulation of cell adhesion; IMP:MGI.
DR GO; GO:0007338; P:single fertilization; IMP:MGI.
DR InterPro; IPR029301; SPACA7.
DR Pfam; PF15307; SPACA7; 1.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Fertilization; Glycoprotein; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..182
FT /note="Sperm acrosome-associated protein 7"
FT /id="PRO_0000350569"
FT REGION 112..154
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 112..138
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 40
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 182 AA; 19943 MW; EFDC925007409BB5 CRC64;
MAANRGSRTF LSVFLLCCWQ GAELQPIKTT SGPITEGSLN STTENIPEAL DEILAQEILE
PKTSAVSETS PRPRSSILTT VQTKEINAGI DENYQEEAFE NYHEVLENIE HLPTKEESGK
NDRSTVANLH DHSSQTKHEP PSSPEGKGSS NDDVYGKLSV LDKILENIGQ SEGSLELTES
IF
