SPAG1_HUMAN
ID SPAG1_HUMAN Reviewed; 926 AA.
AC Q07617; A6NP70; B3KQ58; G3XAM3; Q7Z5G1;
DT 01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 3.
DT 03-AUG-2022, entry version 188.
DE RecName: Full=Sperm-associated antigen 1;
DE AltName: Full=HSD-3.8;
DE AltName: Full=Infertility-related sperm protein Spag-1;
GN Name=SPAG1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, AND VARIANT THR-777.
RC TISSUE=Testis;
RX PubMed=11517287; DOI=10.1093/molehr/7.9.811;
RA Lin W., Zhou X.F., Zhang M.L., Li Y., Miao S.Y., Wang L.F., Zong S.D.,
RA Koide S.S.;
RT "Expression and function of the HSD-3.8 gene encoding a testis-specific
RT protein.";
RL Mol. Hum. Reprod. 7:811-818(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16421571; DOI=10.1038/nature04406;
RA Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M.,
RA Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L.,
RA Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S.,
RA Asakawa T., Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A.,
RA Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III,
RA Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K.,
RA Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B.,
RA O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K.,
RA Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L.,
RA Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G.,
RA Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W.,
RA Platzer M., Shimizu N., Lander E.S.;
RT "DNA sequence and analysis of human chromosome 8.";
RL Nature 439:331-335(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-153.
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PARTIAL NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=1299558;
RA Zhang M.L., Wang L.F., Miao S.Y., Koide S.S.;
RT "Isolation and sequencing of the cDNA encoding the 75-kD human sperm
RT protein related to infertility.";
RL Chin. Med. J. 105:998-1003(1992).
RN [7]
RP TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=16983343; DOI=10.1038/sj.onc.1209961;
RA Neesse A., Gangeswaran R., Luettges J., Feakins R., Weeks M.E.,
RA Lemoine N.R., Crnogorac-Jurcevic T.;
RT "Sperm-associated antigen 1 is expressed early in pancreatic tumorigenesis
RT and promotes motility of cancer cells.";
RL Oncogene 26:1533-1545(2007).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-347 AND SER-791, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [9]
RP INVOLVEMENT IN CILD28, AND TISSUE SPECIFICITY.
RX PubMed=24055112; DOI=10.1016/j.ajhg.2013.07.025;
RA Knowles M.R., Ostrowski L.E., Loges N.T., Hurd T., Leigh M.W., Huang L.,
RA Wolf W.E., Carson J.L., Hazucha M.J., Yin W., Davis S.D., Dell S.D.,
RA Ferkol T.W., Sagel S.D., Olivier K.N., Jahnke C., Olbrich H., Werner C.,
RA Raidt J., Wallmeier J., Pennekamp P., Dougherty G.W., Hjeij R., Gee H.Y.,
RA Otto E.A., Halbritter J., Chaki M., Diaz K.A., Braun D.A., Porath J.D.,
RA Schueler M., Baktai G., Griese M., Turner E.H., Lewis A.P., Bamshad M.J.,
RA Nickerson D.A., Hildebrandt F., Shendure J., Omran H., Zariwala M.A.;
RT "Mutations in SPAG1 cause primary ciliary dyskinesia associated with
RT defective outer and inner dynein arms.";
RL Am. J. Hum. Genet. 93:711-720(2013).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-423, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- FUNCTION: May play a role in the cytoplasmic assembly of the ciliary
CC dynein arms (By similarity). May play a role in fertilization. Binds
CC GTP and has GTPase activity. {ECO:0000250, ECO:0000269|PubMed:11517287,
CC ECO:0000269|PubMed:1299558}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11517287,
CC ECO:0000269|PubMed:16983343}. Dynein axonemal particle {ECO:0000305}.
CC Note=Colocalizes with tubulin.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q07617-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q07617-2; Sequence=VSP_054290, VSP_054291;
CC -!- TISSUE SPECIFICITY: Present in most tissues, including lung, with the
CC strongest expression in brain, colon, kidney, and testis. In sperm and
CC testis, detected in particular in pachytene primary spermatocytes. Up-
CC regulated in pancreatic tumor tissues and not in normal pancreatic
CC tissue. {ECO:0000269|PubMed:11517287, ECO:0000269|PubMed:1299558,
CC ECO:0000269|PubMed:16983343, ECO:0000269|PubMed:24055112}.
CC -!- DISEASE: Ciliary dyskinesia, primary, 28 (CILD28) [MIM:615505]: A
CC disorder characterized by abnormalities of motile cilia. Respiratory
CC infections leading to chronic inflammation and bronchiectasis are
CC recurrent, due to defects in the respiratory cilia. Patients may
CC exhibit randomization of left-right body asymmetry and situs inversus,
CC due to dysfunction of monocilia at the embryonic node. Primary ciliary
CC dyskinesia associated with situs inversus is referred to as Kartagener
CC syndrome. {ECO:0000269|PubMed:24055112}. Note=The disease is caused by
CC variants affecting the gene represented in this entry.
CC -!- MISCELLANEOUS: Antibodies against SPAG1 interfere with fertilization.
CC {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH55091.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305};
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DR EMBL; AF311312; AAG23967.1; -; mRNA.
DR EMBL; AK057482; BAG51920.1; -; mRNA.
DR EMBL; AC025647; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471060; EAW91800.1; -; Genomic_DNA.
DR EMBL; BC055091; AAH55091.1; ALT_SEQ; mRNA.
DR CCDS; CCDS34930.1; -. [Q07617-1]
DR RefSeq; NP_003105.2; NM_003114.4. [Q07617-1]
DR RefSeq; NP_757367.1; NM_172218.2. [Q07617-1]
DR RefSeq; XP_011515544.1; XM_011517242.2.
DR RefSeq; XP_011515545.1; XM_011517243.2. [Q07617-1]
DR PDB; 6I57; NMR; -; A=622-742.
DR PDB; 7BEV; NMR; -; A=206-327.
DR PDBsum; 6I57; -.
DR PDBsum; 7BEV; -.
DR AlphaFoldDB; Q07617; -.
DR SMR; Q07617; -.
DR BioGRID; 112556; 36.
DR ComplexPortal; CPX-6150; R2SP co-chaperone complex.
DR ComplexPortal; CPX-6152; R2SD co-chaperone complex.
DR CORUM; Q07617; -.
DR IntAct; Q07617; 10.
DR MINT; Q07617; -.
DR STRING; 9606.ENSP00000373450; -.
DR iPTMnet; Q07617; -.
DR PhosphoSitePlus; Q07617; -.
DR BioMuta; SPAG1; -.
DR DMDM; 223634718; -.
DR EPD; Q07617; -.
DR jPOST; Q07617; -.
DR MassIVE; Q07617; -.
DR MaxQB; Q07617; -.
DR PaxDb; Q07617; -.
DR PeptideAtlas; Q07617; -.
DR PRIDE; Q07617; -.
DR ProteomicsDB; 33786; -.
DR ProteomicsDB; 58520; -. [Q07617-1]
DR Antibodypedia; 26172; 170 antibodies from 19 providers.
DR DNASU; 6674; -.
DR Ensembl; ENST00000251809.4; ENSP00000251809.3; ENSG00000104450.13. [Q07617-1]
DR Ensembl; ENST00000388798.7; ENSP00000373450.3; ENSG00000104450.13. [Q07617-1]
DR Ensembl; ENST00000520508.5; ENSP00000428070.1; ENSG00000104450.13. [Q07617-2]
DR Ensembl; ENST00000520643.5; ENSP00000427716.1; ENSG00000104450.13. [Q07617-2]
DR GeneID; 6674; -.
DR KEGG; hsa:6674; -.
DR MANE-Select; ENST00000388798.7; ENSP00000373450.3; NM_003114.5; NP_003105.2.
DR UCSC; uc003yjg.2; human. [Q07617-1]
DR CTD; 6674; -.
DR DisGeNET; 6674; -.
DR GeneCards; SPAG1; -.
DR GeneReviews; SPAG1; -.
DR HGNC; HGNC:11212; SPAG1.
DR HPA; ENSG00000104450; Low tissue specificity.
DR MalaCards; SPAG1; -.
DR MIM; 603395; gene.
DR MIM; 615505; phenotype.
DR neXtProt; NX_Q07617; -.
DR OpenTargets; ENSG00000104450; -.
DR Orphanet; 244; Primary ciliary dyskinesia.
DR PharmGKB; PA36049; -.
DR VEuPathDB; HostDB:ENSG00000104450; -.
DR eggNOG; KOG1124; Eukaryota.
DR GeneTree; ENSGT00940000154697; -.
DR HOGENOM; CLU_044641_0_0_1; -.
DR InParanoid; Q07617; -.
DR OMA; QLHRWDG; -.
DR OrthoDB; 1070087at2759; -.
DR PhylomeDB; Q07617; -.
DR TreeFam; TF106251; -.
DR PathwayCommons; Q07617; -.
DR SignaLink; Q07617; -.
DR SIGNOR; Q07617; -.
DR BioGRID-ORCS; 6674; 14 hits in 1069 CRISPR screens.
DR ChiTaRS; SPAG1; human.
DR GeneWiki; SPAG1; -.
DR GenomeRNAi; 6674; -.
DR Pharos; Q07617; Tbio.
DR PRO; PR:Q07617; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q07617; protein.
DR Bgee; ENSG00000104450; Expressed in bronchial epithelial cell and 164 other tissues.
DR Genevisible; Q07617; HS.
DR GO; GO:0101031; C:chaperone complex; IPI:ComplexPortal.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0120293; C:dynein axonemal particle; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0070286; P:axonemal dynein complex assembly; ISS:UniProtKB.
DR GO; GO:0050821; P:protein stabilization; IC:ComplexPortal.
DR GO; GO:0007338; P:single fertilization; TAS:ProtInc.
DR Gene3D; 1.25.40.10; -; 3.
DR InterPro; IPR025986; RPAP3-like_C.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR001440; TPR_1.
DR InterPro; IPR019734; TPR_repeat.
DR Pfam; PF13877; RPAP3_C; 1.
DR Pfam; PF00515; TPR_1; 1.
DR Pfam; PF13181; TPR_8; 1.
DR SMART; SM00028; TPR; 9.
DR SUPFAM; SSF48452; SSF48452; 3.
DR PROSITE; PS50005; TPR; 8.
DR PROSITE; PS50293; TPR_REGION; 4.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Ciliopathy; Cytoplasm; Fertilization;
KW GTP-binding; Hydrolase; Kartagener syndrome; Nucleotide-binding;
KW Phosphoprotein; Primary ciliary dyskinesia; Reference proteome; Repeat;
KW TPR repeat.
FT CHAIN 1..926
FT /note="Sperm-associated antigen 1"
FT /id="PRO_0000106324"
FT REPEAT 209..242
FT /note="TPR 1"
FT REPEAT 244..275
FT /note="TPR 2"
FT REPEAT 276..309
FT /note="TPR 3"
FT REPEAT 445..478
FT /note="TPR 4"
FT REPEAT 487..520
FT /note="TPR 5"
FT REPEAT 522..554
FT /note="TPR 6"
FT REPEAT 623..656
FT /note="TPR 7"
FT REPEAT 657..690
FT /note="TPR 8"
FT REPEAT 692..724
FT /note="TPR 9"
FT REGION 318..452
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 758..801
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 341..366
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 781..788
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
FT MOD_RES 347
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT MOD_RES 354
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q80ZX8"
FT MOD_RES 423
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT MOD_RES 791
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT VAR_SEQ 366..416
FT /note="KPAEPAGAARAAQPCVMGNIQKKLTGKAEGGKRPARGAPQRGQTPEAGADK
FT -> SKIFFLFRLCKKLPFNMMSWLNFSIRTEIRNLSVFLALPCKFTSQFRSSFS (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_054290"
FT VAR_SEQ 417..926
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_054291"
FT VARIANT 331
FT /note="E -> K (in dbSNP:rs17335870)"
FT /id="VAR_054324"
FT VARIANT 777
FT /note="M -> T (in dbSNP:rs6511)"
FT /evidence="ECO:0000269|PubMed:11517287"
FT /id="VAR_054325"
FT VARIANT 827
FT /note="H -> Y (in dbSNP:rs6510)"
FT /id="VAR_054326"
FT CONFLICT 187
FT /note="H -> R (in Ref. 1; AAG23967)"
FT /evidence="ECO:0000305"
FT CONFLICT 195
FT /note="I -> V (in Ref. 2; BAG51920)"
FT /evidence="ECO:0000305"
FT CONFLICT 655
FT /note="K -> T (in Ref. 1; AAG23967)"
FT /evidence="ECO:0000305"
FT HELIX 206..222
FT /evidence="ECO:0007829|PDB:7BEV"
FT HELIX 225..238
FT /evidence="ECO:0007829|PDB:7BEV"
FT HELIX 242..254
FT /evidence="ECO:0007829|PDB:7BEV"
FT HELIX 258..271
FT /evidence="ECO:0007829|PDB:7BEV"
FT HELIX 276..288
FT /evidence="ECO:0007829|PDB:7BEV"
FT HELIX 292..303
FT /evidence="ECO:0007829|PDB:7BEV"
FT HELIX 310..325
FT /evidence="ECO:0007829|PDB:7BEV"
FT HELIX 623..635
FT /evidence="ECO:0007829|PDB:6I57"
FT HELIX 639..652
FT /evidence="ECO:0007829|PDB:6I57"
FT HELIX 657..669
FT /evidence="ECO:0007829|PDB:6I57"
FT HELIX 673..686
FT /evidence="ECO:0007829|PDB:6I57"
FT HELIX 691..703
FT /evidence="ECO:0007829|PDB:6I57"
FT HELIX 707..720
FT /evidence="ECO:0007829|PDB:6I57"
FT HELIX 725..738
FT /evidence="ECO:0007829|PDB:6I57"
SQ SEQUENCE 926 AA; 103639 MW; 947E460842AD9615 CRC64;
MTTKDYPSLW GFGTTKTFKI PIEHLDFKYI EKCSDVKHLE KILCVLRSGE EGYYPELTEF
CEKHLQALAP ESRALRKDKP AATAASFTAE EWEKIDGDIK SWVSEIKKEE DKMHFHETET
FPAMKDNLPP VRGSNSCLHV GKEKYSKRPT KKKTPRDYAE WDKFDVEKEC LKIDEDYKEK
TVIDKSHLSK IETRIDTAGL TEKEKDFLAT REKEKGNEAF NSGDYEEAVM YYTRSISALP
TVVAYNNRAQ AEIKLQNWNS AFQDCEKVLE LEPGNVKALL RRATTYKHQN KLREATEDLS
KVLDVEPDND LAKKTLSEVE RDLKNSEAAS ETQTKGKRMV IQEIENSEDE EGKSGRKHED
GGGDKKPAEP AGAARAAQPC VMGNIQKKLT GKAEGGKRPA RGAPQRGQTP EAGADKRSPR
RASAAAAAGG GATGHPGGGQ GAENPAGLKS QGNELFRSGQ FAEAAGKYSA AIALLEPAGS
EIADDLSILY SNRAACYLKE GNCSGCIQDC NRALELHPFS MKPLLRRAMA YETLEQYGKA
YVDYKTVLQI DCGLQLANDS VNRLSRILME LDGPNWREKL SPIPAVPASV PLQAWHPAKE
MISKQAGDSS SHRQQGITDE KTFKALKEEG NQCVNDKNYK DALSKYSECL KINNKECAIY
TNRALCYLKL CQFEEAKQDC DQALQLADGN VKAFYRRALA HKGLKNYQKS LIDLNKVILL
DPSIIEAKME LEEVTRLLNL KDKTAPFNKE KERRKIEIQE VNEGKEEPGR PAGEVSMGCL
ASEKGGKSSR SPEDPEKLPI AKPNNAYEFG QIINALSTRK DKEACAHLLA ITAPKDLPMF
LSNKLEGDTF LLLIQSLKNN LIEKDPSLVY QHLLYLSKAE RFKMMLTLIS KGQKELIEQL
FEDLSDTPNN HFTLEDIQAL KRQYEL
