SPAG1_RAT
ID SPAG1_RAT Reviewed; 893 AA.
AC Q5U2X2;
DT 01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Sperm-associated antigen 1;
DE AltName: Full=HSD-3.8;
DE AltName: Full=Infertility-related sperm protein Spag-1;
GN Name=Spag1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP FUNCTION.
RX PubMed=11517287; DOI=10.1093/molehr/7.9.811;
RA Lin W., Zhou X.F., Zhang M.L., Li Y., Miao S.Y., Wang L.F., Zong S.D.,
RA Koide S.S.;
RT "Expression and function of the HSD-3.8 gene encoding a testis-specific
RT protein.";
RL Mol. Hum. Reprod. 7:811-818(2001).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-351; SER-703; SER-739 AND
RP SER-740, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: May play a role in the cytoplasmic assembly of the ciliary
CC dynein arms (By similarity). Binds GTP and has GTPase activity (By
CC similarity). Plays a role in fertilization (PubMed:11517287).
CC {ECO:0000250|UniProtKB:Q07617, ECO:0000269|PubMed:11517287}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q07617}. Dynein
CC axonemal particle {ECO:0000250|UniProtKB:Q07617}. Note=Colocalizes with
CC tubulin. {ECO:0000250|UniProtKB:Q07617}.
CC -!- TISSUE SPECIFICITY: Testis and sperm.
CC -!- MISCELLANEOUS: Antibodies against SPAG1 interfere with fertilization.
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DR EMBL; BC085828; AAH85828.1; -; mRNA.
DR RefSeq; NP_001012116.1; NM_001012116.1.
DR AlphaFoldDB; Q5U2X2; -.
DR SMR; Q5U2X2; -.
DR STRING; 10116.ENSRNOP00000013801; -.
DR iPTMnet; Q5U2X2; -.
DR PhosphoSitePlus; Q5U2X2; -.
DR PaxDb; Q5U2X2; -.
DR PRIDE; Q5U2X2; -.
DR Ensembl; ENSRNOT00000013801; ENSRNOP00000013801; ENSRNOG00000010078.
DR GeneID; 315033; -.
DR KEGG; rno:315033; -.
DR UCSC; RGD:1310702; rat.
DR CTD; 6674; -.
DR RGD; 1310702; Spag1.
DR eggNOG; KOG1124; Eukaryota.
DR GeneTree; ENSGT00940000154697; -.
DR HOGENOM; CLU_008405_1_0_1; -.
DR InParanoid; Q5U2X2; -.
DR OMA; QLHRWDG; -.
DR OrthoDB; 1070087at2759; -.
DR PhylomeDB; Q5U2X2; -.
DR TreeFam; TF106251; -.
DR PRO; PR:Q5U2X2; -.
DR Proteomes; UP000002494; Chromosome 7.
DR Bgee; ENSRNOG00000010078; Expressed in testis and 19 other tissues.
DR GO; GO:0101031; C:chaperone complex; ISO:RGD.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0120293; C:dynein axonemal particle; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0070286; P:axonemal dynein complex assembly; ISS:UniProtKB.
DR GO; GO:0007338; P:single fertilization; IEA:UniProtKB-KW.
DR Gene3D; 1.25.40.10; -; 3.
DR InterPro; IPR025986; RPAP3-like_C.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR001440; TPR_1.
DR InterPro; IPR019734; TPR_repeat.
DR Pfam; PF13877; RPAP3_C; 1.
DR Pfam; PF00515; TPR_1; 2.
DR Pfam; PF13181; TPR_8; 1.
DR SMART; SM00028; TPR; 8.
DR SUPFAM; SSF48452; SSF48452; 3.
DR PROSITE; PS50005; TPR; 5.
DR PROSITE; PS50293; TPR_REGION; 4.
PE 1: Evidence at protein level;
KW Cytoplasm; Fertilization; GTP-binding; Hydrolase; Nucleotide-binding;
KW Phosphoprotein; Reference proteome; Repeat; TPR repeat.
FT CHAIN 1..893
FT /note="Sperm-associated antigen 1"
FT /id="PRO_0000106326"
FT REPEAT 213..246
FT /note="TPR 1"
FT REPEAT 247..279
FT /note="TPR 2"
FT REPEAT 280..313
FT /note="TPR 3"
FT REPEAT 429..463
FT /note="TPR 4"
FT REPEAT 471..504
FT /note="TPR 5"
FT REPEAT 506..538
FT /note="TPR 6"
FT REPEAT 605..638
FT /note="TPR 7"
FT REPEAT 639..672
FT /note="TPR 8"
FT REGION 112..155
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 324..344
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 349..368
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 373..437
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 704..756
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 112..128
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 353..367
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 415..431
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 709..736
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 630..637
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
FT MOD_RES 351
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 703
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 739
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 740
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 758
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q07617"
SQ SEQUENCE 893 AA; 100615 MW; E95507EFF0B3A49F CRC64;
MTAKVKDHPP LWGFGTTKTF KIPIEHLDFK YIENCSDVKQ LEKILCVLRS GEEGYYPELT
EFCEKRLTGL APRSRALRKD KPAATASSFS AEEWEKIDSD LKSWVSEIKR EENTRHFHDP
EKHPGVEDPL PPVRGSNSCP RGGKETSSKS KTAKKRIPRD YAEWDKFDVE KECSKIDEDY
KEKTVINNKA HLSKIETKID TAGLTEKEKN FLANREKGKG NEAFYSGDYE EAVMYYTRSL
SALPTATAYN NRAQAEIKLQ RWSSALEDCE KALELEPGNI KALLRRATTY KHQNKFLEAV
DDLRKVLQAE PDNDLAKKTL SEVERELKNS EPASELQTKG KRMVIEEVEN SGDEGGKGDE
DDHEDDGVDM AAMGNIQKKL MVRSQGGRRS RRARTPMPGA EQQEGQPETG TASTSDNHDL
EERRAADSPG DLKSRGNELF RGGQFAEAAV QYSGAIAQLE PTGSENADEL SILYSNRAAC
YLKEGNCRGC IQDCDRALEL QPFAVKPLLR RAMAYETLEQ YRSAYVDYIT VLKIDCRIQL
ASDSVNRITR ILTELDGPKW RERLPPIPAV PASEPLRVWH PAAETPDQDP CPNSCTPTIT
DEKMFQALKE EGNQLVKDKN YKDAISKYNE CLKINSKACA IYTNRALCYL KLGQFEEAKL
DCDKALQIDS KNVKASYRLE LAQKGLENCR ERVADPSQVV LLSPDSSEAA RHLDTKNDTA
PPSRERERRR IEIQEVDDSS DEEPERPAEA SAVEEGWSAE RAGKIEVCKP RNAYEFGQVL
STISARKDEE ACAQLLVFTA PQDLPVLLSN KLEGDMLLLI MQSLKSHLVA KDPSLVCKHL
LYLSKAERFE MMLALTSKDQ KEQMAQLFDD LSDAQADCLT AEDIQALRRQ YVL
