SPAG6_MOUSE
ID SPAG6_MOUSE Reviewed; 507 AA.
AC Q9JLI7; Q8K461;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Sperm-associated antigen 6;
DE AltName: Full=Axoneme central apparatus protein;
DE AltName: Full=Protein PF16 homolog;
GN Name=Spag6; Synonyms=Pf16;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND SUBCELLULAR
RP LOCATION.
RC TISSUE=Sperm;
RX PubMed=10684790; DOI=10.1095/biolreprod62.3.511;
RA Sapiro R., Tarantino L.M., Velazquez F., Kiriakidou M., Hecht N.B.,
RA Bucan M., Strauss J.F. III;
RT "Sperm antigen 6 is the murine homologue of the Chlamydomonas reinhardtii
RT central apparatus protein encoded by the PF16 locus.";
RL Biol. Reprod. 62:511-518(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 42-96, FUNCTION, AND DISEASE.
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=12167721; DOI=10.1128/mcb.22.17.6298-6305.2002;
RA Sapiro R., Kostetskii I., Olds-Clarke P., Gerton G.L., Radice G.L.,
RA Strauss J.F. III;
RT "Male infertility, impaired sperm motility, and hydrocephalus in mice
RT deficient in sperm-associated antigen 6.";
RL Mol. Cell. Biol. 22:6298-6305(2002).
RN [4]
RP INTERACTION WITH SPAG16, AND TISSUE SPECIFICITY.
RC TISSUE=Sperm;
RX PubMed=12391165; DOI=10.1128/mcb.22.22.7993-8004.2002;
RA Zhang Z., Sapiro R., Kapfhamer D., Bucan M., Bray J., Chennathukuzhi V.,
RA McNamara P., Curtis A., Zhang M., Blanchette-Mackie E.J., Strauss J.F. III;
RT "A sperm-associated WD repeat protein orthologous to Chlamydomonas PF20
RT associates with Spag6, the mammalian orthologue of Chlamydomonas PF16.";
RL Mol. Cell. Biol. 22:7993-8004(2002).
RN [5]
RP INTERACTION WITH SPAG17, AND DISRUPTION PHENOTYPE.
RC STRAIN=C57BL/6J;
RX PubMed=15827353; DOI=10.1074/mcp.m400177-mcp200;
RA Zhang Z., Jones B.H., Tang W., Moss S.B., Wei Z., Ho C., Pollack M.,
RA Horowitz E., Bennett J., Baker M.E., Strauss J.F. III;
RT "Dissecting the axoneme interactome: the mammalian orthologue of
RT Chlamydomonas PF6 interacts with sperm-associated antigen 6, the mammalian
RT orthologue of Chlamydomonas PF16.";
RL Mol. Cell. Proteomics 4:914-923(2005).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=30535028; DOI=10.1093/jmcb/mjy014;
RA Zheng J., Liu H., Zhu L., Chen Y., Zhao H., Zhang W., Li F., Xie L.,
RA Yan X., Zhu X.;
RT "Microtubule-bundling protein Spef1 enables mammalian ciliary central
RT apparatus formation.";
RL J. Mol. Cell Biol. 11:67-77(2019).
CC -!- FUNCTION: Important for structural integrity of the central apparatus
CC in the sperm tail and for flagellar motility.
CC {ECO:0000269|PubMed:10684790, ECO:0000269|PubMed:12167721}.
CC -!- SUBUNIT: Interacts with SPAG16 AND SPAG17.
CC {ECO:0000269|PubMed:12391165, ECO:0000269|PubMed:15827353}.
CC -!- INTERACTION:
CC Q9JLI7; Q5S003: Spag17; NbExp=3; IntAct=EBI-1783654, EBI-1783665;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000269|PubMed:10684790}. Cell projection, cilium, flagellum
CC {ECO:0000269|PubMed:10684790}. Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000269|PubMed:30535028}. Note=Associated with microtubules.
CC Detected on the sperm flagellum (PubMed:10684790). Localizes in the
CC cilium axoneme in a SPEF1-dependent manner (PubMed:30535028).
CC {ECO:0000269|PubMed:10684790, ECO:0000269|PubMed:30535028}.
CC -!- TISSUE SPECIFICITY: Highly expressed in testis. Not detected in
CC prostate, ovary, spleen, thymus, small intestine, colon and peripheral
CC blood leukocytes. {ECO:0000269|PubMed:10684790,
CC ECO:0000269|PubMed:12391165}.
CC -!- DISEASE: Note=Defects in Spag6 are a cause of hydrocephalus and of male
CC infertility. {ECO:0000269|PubMed:12167721}.
CC -!- DISRUPTION PHENOTYPE: In null embryos the central apparatus is unstable
CC leading to a loss, and presumably degradation of proteins to which
CC SPAG6 normally binds, including SPAG16 AND SPAG17.
CC {ECO:0000269|PubMed:15827353}.
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DR EMBL; AF173866; AAF35831.1; -; mRNA.
DR EMBL; AK005732; BAB24211.1; -; mRNA.
DR EMBL; AF486266; AAM64149.1; -; Genomic_DNA.
DR CCDS; CCDS27988.1; -.
DR RefSeq; NP_056588.1; NM_015773.2.
DR AlphaFoldDB; Q9JLI7; -.
DR SMR; Q9JLI7; -.
DR BioGRID; 206052; 1.
DR IntAct; Q9JLI7; 1.
DR STRING; 10090.ENSMUSP00000023468; -.
DR PhosphoSitePlus; Q9JLI7; -.
DR MaxQB; Q9JLI7; -.
DR PaxDb; Q9JLI7; -.
DR PRIDE; Q9JLI7; -.
DR ProteomicsDB; 261490; -.
DR Ensembl; ENSMUST00000023468; ENSMUSP00000023468; ENSMUSG00000022783.
DR GeneID; 50525; -.
DR KEGG; mmu:50525; -.
DR UCSC; uc007yjb.2; mouse.
DR CTD; 50525; -.
DR MGI; MGI:1354388; Spag6.
DR VEuPathDB; HostDB:ENSMUSG00000022783; -.
DR eggNOG; KOG0166; Eukaryota.
DR GeneTree; ENSGT00900000141099; -.
DR HOGENOM; CLU_022627_1_0_1; -.
DR InParanoid; Q9JLI7; -.
DR OMA; GRHTSEH; -.
DR OrthoDB; 577008at2759; -.
DR PhylomeDB; Q9JLI7; -.
DR TreeFam; TF328894; -.
DR BioGRID-ORCS; 50525; 0 hits in 15 CRISPR screens.
DR ChiTaRS; Spag6; mouse.
DR PRO; PR:Q9JLI7; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q9JLI7; protein.
DR Bgee; ENSMUSG00000022783; Expressed in spermatocyte and 62 other tissues.
DR ExpressionAtlas; Q9JLI7; baseline and differential.
DR Genevisible; Q9JLI7; MM.
DR GO; GO:0001669; C:acrosomal vesicle; IDA:MGI.
DR GO; GO:1990716; C:axonemal central apparatus; IGI:MGI.
DR GO; GO:0005930; C:axoneme; IDA:UniProtKB.
DR GO; GO:0031410; C:cytoplasmic vesicle; IDA:MGI.
DR GO; GO:0005829; C:cytosol; IDA:MGI.
DR GO; GO:0005576; C:extracellular region; IEA:GOC.
DR GO; GO:0120249; C:lateral wall of outer hair cell; IDA:MGI.
DR GO; GO:0002177; C:manchette; IDA:MGI.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0015630; C:microtubule cytoskeleton; ISO:MGI.
DR GO; GO:0097228; C:sperm principal piece; IDA:MGI.
DR GO; GO:0008017; F:microtubule binding; IDA:MGI.
DR GO; GO:0051301; P:cell division; IMP:MGI.
DR GO; GO:0090660; P:cerebrospinal fluid circulation; IMP:MGI.
DR GO; GO:0003341; P:cilium movement; IBA:GO_Central.
DR GO; GO:0043583; P:ear development; IMP:MGI.
DR GO; GO:0003351; P:epithelial cilium movement involved in extracellular fluid movement; IMP:MGI.
DR GO; GO:0045198; P:establishment of epithelial cell apical/basal polarity; IMP:MGI.
DR GO; GO:0051649; P:establishment of localization in cell; IMP:MGI.
DR GO; GO:0010761; P:fibroblast migration; IMP:MGI.
DR GO; GO:0046847; P:filopodium assembly; IMP:MGI.
DR GO; GO:0030317; P:flagellated sperm motility; IMP:MGI.
DR GO; GO:0044458; P:motile cilium assembly; IMP:MGI.
DR GO; GO:0120197; P:mucociliary clearance; IGI:MGI.
DR GO; GO:1990138; P:neuron projection extension; IMP:MGI.
DR GO; GO:1905515; P:non-motile cilium assembly; IMP:MGI.
DR GO; GO:0008104; P:protein localization; IMP:MGI.
DR GO; GO:0003356; P:regulation of cilium beat frequency; IMP:MGI.
DR GO; GO:0090175; P:regulation of establishment of planar polarity; IMP:MGI.
DR GO; GO:0007288; P:sperm axoneme assembly; IMP:MGI.
DR GO; GO:0007283; P:spermatogenesis; IMP:MGI.
DR GO; GO:0043149; P:stress fiber assembly; IMP:MGI.
DR GO; GO:0006931; P:substrate-dependent cell migration, cell attachment to substrate; IMP:MGI.
DR GO; GO:0021591; P:ventricular system development; IMP:MGI.
DR Gene3D; 1.25.10.10; -; 2.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR Pfam; PF00514; Arm; 4.
DR SMART; SM00185; ARM; 7.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Cilium biogenesis/degradation; Cytoplasm;
KW Cytoskeleton; Flagellum; Microtubule; Reference proteome; Repeat.
FT CHAIN 1..507
FT /note="Sperm-associated antigen 6"
FT /id="PRO_0000072096"
FT REPEAT 31..70
FT /note="ARM 1"
FT REPEAT 73..112
FT /note="ARM 2"
FT REPEAT 115..154
FT /note="ARM 3"
FT REPEAT 157..196
FT /note="ARM 4"
FT REPEAT 199..238
FT /note="ARM 5"
FT REPEAT 241..280
FT /note="ARM 6"
FT REPEAT 325..365
FT /note="ARM 7"
FT REPEAT 402..441
FT /note="ARM 8"
SQ SEQUENCE 507 AA; 55269 MW; EFFAE719EC111285 CRC64;
MSQRQVLQVF EQYQKARTQF VQMVAELATR PQNIETLQNA GVMSLLRPLL LDVVPTIQQT
AALALGRLAN YNDDLAEAVV KGDILPQLVY SLAEQNRFYK KAAAFVLRAV GKHSPQLAQA
IVDCGALDTL VICLEDFDPG VKEAAAWALG YIARHNTELS QAVVDAGAIP LLVLCIQEPE
IALKRIAASA LSDISKHSPE LAQTVVDAGA IAHLAQMILN PDAKLKRQVL SALSQIAKHS
VDLAEMVVEA EIFPVVLTCL KDKDEYVKKN ACTLIREIAK HTPELSQLIV NAGGVAAVID
CIGSCKGNIR LPGIMMLGYV AAHSENLAMA VIISKGVPQL SICLSEEPED HIKAAAAWAL
GQLGRHTPEH ARAVAVTNTL PVLLSLYMSP ESSEDLQLKS KKAIKNILQK CTYLPALEPF
LYDAPPNILK HVVGQFSKVL PHDSKARRLF VTSGGLKKVQ EIKAEPGSLL QEYINSINNC
YPEEIVRYYS PGYSDTLLQR VDSYQPL
