SPAG7_MOUSE
ID SPAG7_MOUSE Reviewed; 227 AA.
AC Q7TNE3; Q3TM27; Q80XJ8; Q8BK16;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Sperm-associated antigen 7;
GN Name=Spag7;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain, and Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114 AND SER-202, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Lung, Pancreas, Spleen,
RC and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
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DR EMBL; AK042294; BAE20629.1; -; mRNA.
DR EMBL; AK077543; BAC36855.1; -; mRNA.
DR EMBL; AK166184; BAE38615.1; -; mRNA.
DR EMBL; AL596117; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC046751; AAH46751.1; -; mRNA.
DR EMBL; BC055821; AAH55821.1; -; mRNA.
DR CCDS; CCDS24962.1; -.
DR RefSeq; NP_001161141.1; NM_001167669.1.
DR RefSeq; NP_766149.2; NM_172561.3.
DR AlphaFoldDB; Q7TNE3; -.
DR SMR; Q7TNE3; -.
DR BioGRID; 229814; 1.
DR STRING; 10090.ENSMUSP00000018431; -.
DR iPTMnet; Q7TNE3; -.
DR PhosphoSitePlus; Q7TNE3; -.
DR EPD; Q7TNE3; -.
DR jPOST; Q7TNE3; -.
DR MaxQB; Q7TNE3; -.
DR PaxDb; Q7TNE3; -.
DR PeptideAtlas; Q7TNE3; -.
DR PRIDE; Q7TNE3; -.
DR ProteomicsDB; 261560; -.
DR Antibodypedia; 5775; 80 antibodies from 22 providers.
DR Ensembl; ENSMUST00000018431; ENSMUSP00000018431; ENSMUSG00000018287.
DR GeneID; 216873; -.
DR KEGG; mmu:216873; -.
DR UCSC; uc007jvz.2; mouse.
DR CTD; 9552; -.
DR MGI; MGI:107380; Spag7.
DR VEuPathDB; HostDB:ENSMUSG00000018287; -.
DR eggNOG; ENOG502QW1E; Eukaryota.
DR GeneTree; ENSGT00390000001520; -.
DR HOGENOM; CLU_091198_0_0_1; -.
DR InParanoid; Q7TNE3; -.
DR OMA; HRSVIHE; -.
DR OrthoDB; 1416114at2759; -.
DR PhylomeDB; Q7TNE3; -.
DR TreeFam; TF323631; -.
DR BioGRID-ORCS; 216873; 7 hits in 72 CRISPR screens.
DR ChiTaRS; Spag7; mouse.
DR PRO; PR:Q7TNE3; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q7TNE3; protein.
DR Bgee; ENSMUSG00000018287; Expressed in retinal neural layer and 263 other tissues.
DR ExpressionAtlas; Q7TNE3; baseline and differential.
DR Genevisible; Q7TNE3; MM.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR CDD; cd02636; R3H_sperm-antigen; 1.
DR Gene3D; 3.30.1370.50; -; 1.
DR InterPro; IPR001374; R3H_dom.
DR InterPro; IPR036867; R3H_dom_sf.
DR InterPro; IPR034068; R3H_sperm-antigen.
DR InterPro; IPR017330; SPAG7.
DR PANTHER; PTHR13498; PTHR13498; 1.
DR Pfam; PF01424; R3H; 1.
DR PIRSF; PIRSF037943; Sperm-assoc_antigen_PAG7; 1.
DR SMART; SM00393; R3H; 1.
DR SUPFAM; SSF82708; SSF82708; 1.
DR PROSITE; PS51061; R3H; 1.
PE 1: Evidence at protein level;
KW Acetylation; Nucleus; Phosphoprotein; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:O75391"
FT CHAIN 2..227
FT /note="Sperm-associated antigen 7"
FT /id="PRO_0000072098"
FT DOMAIN 46..109
FT /note="R3H"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00382"
FT REGION 1..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 118..161
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 35..51
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOTIF 122..139
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 18..45
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 118..146
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:O75391"
FT MOD_RES 114
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 158
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O75391"
FT MOD_RES 202
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 163
FT /note="Y -> N (in Ref. 1; BAC36855)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 227 AA; 25921 MW; A80FB02CEC2305EF CRC64;
MADLLGSILS SMEKPPSLGD QESRRKAREQ AARLKKLQEQ DKQQKVEFRK RMEKEVSDFI
QDSGQVKKKF QPMNKIERSI LHDVVEVAGL TSFSFGEDDD CRYVMIFKKE FAPSDEELDS
YRHGEEWDPQ KAEEKRKLKE LAQKQEEEAA QQGPAVVSPA SDYKDKYSHL IGKGAAKDAA
HMLQANKTYG CVPVANKRDT RSIEEAMNEI RAKKRLRQSG EELPTTS
