SPAG8_BOVIN
ID SPAG8_BOVIN Reviewed; 484 AA.
AC E1BNS6;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 10-APR-2019, sequence version 3.
DT 03-AUG-2022, entry version 54.
DE RecName: Full=Sperm-associated antigen 8;
GN Name=SPAG8;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913 {ECO:0000312|Proteomes:UP000009136};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hereford;
RX PubMed=19393038; DOI=10.1186/gb-2009-10-4-r42;
RA Zimin A.V., Delcher A.L., Florea L., Kelley D.R., Schatz M.C., Puiu D.,
RA Hanrahan F., Pertea G., Van Tassell C.P., Sonstegard T.S., Marcais G.,
RA Roberts M., Subramanian P., Yorke J.A., Salzberg S.L.;
RT "A whole-genome assembly of the domestic cow, Bos taurus.";
RL Genome Biol. 10:R42.01-R42.10(2009).
RN [2] {ECO:0007744|PDB:7RRO}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.40 ANGSTROMS), AND SUBCELLULAR
RP LOCATION.
RX PubMed=34715025; DOI=10.1016/j.cell.2021.10.007;
RA Gui M., Farley H., Anujan P., Anderson J.R., Maxwell D.W., Whitchurch J.B.,
RA Botsch J.J., Qiu T., Meleppattu S., Singh S.K., Zhang Q., Thompson J.,
RA Lucas J.S., Bingle C.D., Norris D.P., Roy S., Brown A.;
RT "De novo identification of mammalian ciliary motility proteins using cryo-
RT EM.";
RL Cell 184:5791-5806.e19(2021).
CC -!- FUNCTION: Plays a role in spermatogenesis by enhancing the binding of
CC CREM isoform tau to its coactivator FHL5 and increasing the FHL5-
CC regulated transcriptional activation of CREM isoform tau (By
CC similarity). Involved in the acrosome reaction and in binding of sperm
CC to the zona pellucida (By similarity). Plays a role in regulation of
CC the cell cycle by controlling progression through the G2/M phase,
CC possibly by delaying the activation of CDK1 which is required for entry
CC into mitosis. May play a role in fertility and microtubule formation
CC through interaction with RANBP9 (By similarity).
CC {ECO:0000250|UniProtKB:Q3V0Q6, ECO:0000250|UniProtKB:Q99932}.
CC -!- SUBUNIT: Interacts with FHL5 (via second LIM domain) (By similarity).
CC Interacts with RANBP9 (By similarity). {ECO:0000250|UniProtKB:Q3V0Q6,
CC ECO:0000250|UniProtKB:Q99932}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q3V0Q6}. Nucleus
CC {ECO:0000250|UniProtKB:Q3V0Q6}. Cytoplasmic vesicle, secretory vesicle,
CC acrosome {ECO:0000250|UniProtKB:Q99932}. Cytoplasm, cytoskeleton,
CC microtubule organizing center {ECO:0000250|UniProtKB:Q99932}.
CC Cytoplasm, cytoskeleton, spindle {ECO:0000250|UniProtKB:Q99932}.
CC Cytoplasm, cytoskeleton, cilium axoneme {ECO:0000269|PubMed:34715025}.
CC Note=In mature sperm cells, detected in the acrosomal region of the
CC head and in the middle piece of the tail (By similarity). Localized to
CC the nucleus and cytoplasm of spermatocytes and round spermatids while,
CC in elongating spermatids, expressed in the cytoplasm but not in the
CC nucleus (By similarity). During the cell cycle, localized on the
CC microtubule-organizing center (MTOC) during prophase. In metaphase,
CC extends along spindle microtubules. In anaphase, detected on the astral
CC microtubules and mid-zone. In telophase, remains at the mid-zone. After
CC cytokinesis, returns to the MTOC (By similarity). Microtubule inner
CC protein (MIP) part of the dynein-decorated doublet microtubules (DMTs)
CC in cilia axoneme (PubMed:34715025). {ECO:0000250|UniProtKB:Q3V0Q6,
CC ECO:0000250|UniProtKB:Q99932, ECO:0000269|PubMed:34715025}.
CC -!- TISSUE SPECIFICITY: Expressed in trachea multiciliated cells.
CC {ECO:0000269|PubMed:34715025}.
CC -!- SIMILARITY: Belongs to the SPAG8 family. {ECO:0000305}.
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DR RefSeq; XP_005210137.1; XM_005210080.2.
DR PDB; 7RRO; EM; 3.40 A; D=1-484.
DR PDBsum; 7RRO; -.
DR STRING; 9913.ENSBTAP00000015206; -.
DR PaxDb; E1BNS6; -.
DR Ensembl; ENSBTAT00000015206.5; ENSBTAP00000015206.5; ENSBTAG00000011442.6.
DR GeneID; 514627; -.
DR CTD; 26206; -.
DR VEuPathDB; HostDB:ENSBTAG00000011442; -.
DR VGNC; VGNC:35169; SPAG8.
DR eggNOG; ENOG502S06E; Eukaryota.
DR GeneTree; ENSGT00640000091617; -.
DR HOGENOM; CLU_052284_0_0_1; -.
DR InParanoid; E1BNS6; -.
DR OMA; HCLLYNW; -.
DR OrthoDB; 1153567at2759; -.
DR TreeFam; TF329075; -.
DR Proteomes; UP000009136; Chromosome 8.
DR Bgee; ENSBTAG00000011442; Expressed in laryngeal cartilage and 103 other tissues.
DR GO; GO:0005879; C:axonemal microtubule; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0001673; C:male germ cell nucleus; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0008017; F:microtubule binding; IEA:InterPro.
DR GO; GO:0032092; P:positive regulation of protein binding; IEA:Ensembl.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR026124; Sperm-assoc_Ag8.
DR PANTHER; PTHR15510; PTHR15510; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; Cell projection; Cytoplasm; Cytoplasmic vesicle;
KW Cytoskeleton; Differentiation; Fertilization; Nucleus; Reference proteome;
KW Spermatogenesis.
FT CHAIN 1..484
FT /note="Sperm-associated antigen 8"
FT /id="PRO_0000456165"
SQ SEQUENCE 484 AA; 51542 MW; 4E21682D8C06DBC8 CRC64;
METSESTDRS QSRCLDLQPS SDGLGSSSDP FSSWDGRHRS ALVAATAAAS AAATAASTAR
AAALWTKSPA PYSHGNLLTE PSSDSLTERY TGPRFTHKIS HGRLGFQPAY FSHIAWNPYT
TNDLSSSRGP IPGSSSGPVP GSSSSPGPDS SSDPGPSSSS GPGGSPGGSG RGPGHGPGPG
GGSGQGPGGG SGQGTDLGPA IDSRHSPGHG HGPRFNFSAP VGFRNPRGDL IPNYTGCKHH
CHWEPQKQSW KFLKVSEPGA RGLWKPPEVE GKSTVLSETL PRGQCLLYNW EEERATNYLD
QVPVMQDGSE SFFFRHGHRG LLTLQPQSPT SSCTTQKDSY QPPKSHCQPI RGKREAILEM
LLRQQICKEV QAEQEPTRKD SEVESVTHHD YKKELVQAGP PAPTKIHDYH TEQPETFWLE
RAPQLPGVSN IRTLDTPFRK NCSFSTPVPL SLEQPLPFEP ESYSQHGEIS SLACQGGGQG
GGGG
