SPAG8_HUMAN
ID SPAG8_HUMAN Reviewed; 485 AA.
AC Q99932; B4DY89; E9PDV6; Q12937; Q5TCV8; Q8WWB4;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 16-JAN-2019, sequence version 3.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Sperm-associated antigen 8;
DE AltName: Full=HSD-1;
DE AltName: Full=Sperm membrane protein 1;
DE Short=SMP-1;
DE AltName: Full=Sperm membrane protein BS-84;
GN Name=SPAG8 {ECO:0000312|HGNC:HGNC:14105};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND SUBCELLULAR
RP LOCATION.
RC TISSUE=Testis;
RX PubMed=8788182; DOI=10.1095/biolreprod54.2.323;
RA Liu Q.-Y., Wang L.F., Miao S.Y., Catterall J.F.;
RT "Expression and characterization of a novel human sperm membrane protein.";
RL Biol. Reprod. 54:323-330(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING, AND FUNCTION.
RC TISSUE=Testis;
RX PubMed=10500252; DOI=10.1016/s0167-4781(99)00136-0;
RA Wang H., Miao S.Y., Chen D., Wang L.F., Koide S.S.;
RT "Assignment of chromosomal locus and evidence for alternatively spliced
RT mRNAs of a human sperm membrane protein (hSMP-1).";
RL Biochim. Biophys. Acta 1447:119-124(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANT LEU-226.
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP INTERACTION WITH RANBP9, AND SUBCELLULAR LOCATION.
RC TISSUE=Testis;
RX PubMed=15014887; DOI=10.1007/s00109-004-0535-2;
RA Tang X., Zhang J., Cai Y., Miao S.Y., Zong S., Koide S.S., Wang L.F.;
RT "Sperm membrane protein (hSMP-1) and RanBPM complex in the microtubule-
RT organizing centre.";
RL J. Mol. Med. 82:383-388(2004).
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=17187156; DOI=10.1111/j.1745-7262.2007.00247.x;
RA Cheng G.Y., Shi J.L., Wang M., Hu Y.Q., Liu C.M., Wang Y.F., Xu C.;
RT "Inhibition of mouse acrosome reaction and sperm-zona pellucida binding by
RT anti-human sperm membrane protein 1 antibody.";
RL Asian J. Androl. 9:23-29(2007).
RN [8]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=19548270; DOI=10.1002/cbf.1574;
RA Li R., Tang X.L., Miao S.Y., Zong S.D., Wang L.F.;
RT "Regulation of the G2/M phase of the cell cycle by sperm associated antigen
RT 8 (SPAG8) protein.";
RL Cell Biochem. Funct. 27:264-268(2009).
CC -!- FUNCTION: Plays a role in spermatogenesis by enhancing the binding of
CC CREM isoform tau to its coactivator FHL5 and increasing the FHL5-
CC regulated transcriptional activation of CREM isoform tau (By
CC similarity). Involved in the acrosome reaction and in binding of sperm
CC to the zona pellucida (By similarity). Plays a role in regulation of
CC the cell cycle by controlling progression through the G2/M phase,
CC possibly by delaying the activation of CDK1 which is required for entry
CC into mitosis (PubMed:19548270). May play a role in fertility and
CC microtubule formation through interaction with RANBP9
CC (PubMed:10500252). {ECO:0000250|UniProtKB:Q3V0Q6,
CC ECO:0000269|PubMed:10500252, ECO:0000269|PubMed:19548270}.
CC -!- SUBUNIT: Interacts with FHL5 (via second LIM domain) (By similarity).
CC Interacts with RANBP9 (PubMed:15014887). {ECO:0000250|UniProtKB:Q3V0Q6,
CC ECO:0000269|PubMed:15014887}.
CC -!- INTERACTION:
CC Q99932; P33240: CSTF2; NbExp=3; IntAct=EBI-954419, EBI-711360;
CC Q99932; Q86UW9: DTX2; NbExp=3; IntAct=EBI-954419, EBI-740376;
CC Q99932; Q5THT1: KLHL32; NbExp=3; IntAct=EBI-954419, EBI-10247181;
CC Q99932; Q5TAL4: SNRPC; NbExp=3; IntAct=EBI-954419, EBI-10246938;
CC Q99932-2; P05067: APP; NbExp=3; IntAct=EBI-11959123, EBI-77613;
CC Q99932-2; P05067-2: APP; NbExp=3; IntAct=EBI-11959123, EBI-17264467;
CC Q99932-2; Q96C12: ARMC5; NbExp=3; IntAct=EBI-11959123, EBI-6425121;
CC Q99932-2; P54253: ATXN1; NbExp=6; IntAct=EBI-11959123, EBI-930964;
CC Q99932-2; P54252: ATXN3; NbExp=6; IntAct=EBI-11959123, EBI-946046;
CC Q99932-2; Q9BPU9: B9D2; NbExp=3; IntAct=EBI-11959123, EBI-6958971;
CC Q99932-2; Q8N1L9: BATF2; NbExp=3; IntAct=EBI-11959123, EBI-742695;
CC Q99932-2; J3KQ12: BSCL2; NbExp=3; IntAct=EBI-11959123, EBI-11532900;
CC Q99932-2; Q5BKX5-3: C19orf54; NbExp=4; IntAct=EBI-11959123, EBI-11976299;
CC Q99932-2; Q96HB5: CCDC120; NbExp=3; IntAct=EBI-11959123, EBI-744556;
CC Q99932-2; P24863: CCNC; NbExp=3; IntAct=EBI-11959123, EBI-395261;
CC Q99932-2; P48643: CCT5; NbExp=3; IntAct=EBI-11959123, EBI-355710;
CC Q99932-2; Q9UJX2: CDC23; NbExp=3; IntAct=EBI-11959123, EBI-396137;
CC Q99932-2; P27918: CFP; NbExp=4; IntAct=EBI-11959123, EBI-9038570;
CC Q99932-2; Q8N5K1: CISD2; NbExp=3; IntAct=EBI-11959123, EBI-1045797;
CC Q99932-2; P33240: CSTF2; NbExp=3; IntAct=EBI-11959123, EBI-711360;
CC Q99932-2; Q9H0L4: CSTF2T; NbExp=3; IntAct=EBI-11959123, EBI-747012;
CC Q99932-2; P78358: CTAG1B; NbExp=5; IntAct=EBI-11959123, EBI-1188472;
CC Q99932-2; Q14203-5: DCTN1; NbExp=3; IntAct=EBI-11959123, EBI-25840379;
CC Q99932-2; Q9NQL9: DMRT3; NbExp=3; IntAct=EBI-11959123, EBI-9679045;
CC Q99932-2; G5E9A7: DMWD; NbExp=3; IntAct=EBI-11959123, EBI-10976677;
CC Q99932-2; Q7L591-3: DOK3; NbExp=3; IntAct=EBI-11959123, EBI-10694655;
CC Q99932-2; Q96FJ2: DYNLL2; NbExp=3; IntAct=EBI-11959123, EBI-742371;
CC Q99932-2; Q9UHF1: EGFL7; NbExp=3; IntAct=EBI-11959123, EBI-949532;
CC Q99932-2; A1KXE4-2: FAM168B; NbExp=3; IntAct=EBI-11959123, EBI-12193763;
CC Q99932-2; Q8WU58: FAM222B; NbExp=3; IntAct=EBI-11959123, EBI-2807642;
CC Q99932-2; O43559: FRS3; NbExp=3; IntAct=EBI-11959123, EBI-725515;
CC Q99932-2; P41250: GARS1; NbExp=3; IntAct=EBI-11959123, EBI-724143;
CC Q99932-2; O75420: GIGYF1; NbExp=3; IntAct=EBI-11959123, EBI-947774;
CC Q99932-2; Q8IVS8: GLYCTK; NbExp=3; IntAct=EBI-11959123, EBI-748515;
CC Q99932-2; Q9Y4H4: GPSM3; NbExp=3; IntAct=EBI-11959123, EBI-347538;
CC Q99932-2; Q9Y5R4: HEMK1; NbExp=3; IntAct=EBI-11959123, EBI-10329202;
CC Q99932-2; O43593: HR; NbExp=3; IntAct=EBI-11959123, EBI-2880706;
CC Q99932-2; P04792: HSPB1; NbExp=3; IntAct=EBI-11959123, EBI-352682;
CC Q99932-2; P42858: HTT; NbExp=18; IntAct=EBI-11959123, EBI-466029;
CC Q99932-2; Q0VD86: INCA1; NbExp=3; IntAct=EBI-11959123, EBI-6509505;
CC Q99932-2; Q5TA45: INTS11; NbExp=3; IntAct=EBI-11959123, EBI-748258;
CC Q99932-2; O60333-2: KIF1B; NbExp=3; IntAct=EBI-11959123, EBI-10975473;
CC Q99932-2; O76011: KRT34; NbExp=3; IntAct=EBI-11959123, EBI-1047093;
CC Q99932-2; P60410: KRTAP10-8; NbExp=3; IntAct=EBI-11959123, EBI-10171774;
CC Q99932-2; Q8IUC1: KRTAP11-1; NbExp=3; IntAct=EBI-11959123, EBI-1052037;
CC Q99932-2; Q3SY46: KRTAP13-3; NbExp=3; IntAct=EBI-11959123, EBI-10241252;
CC Q99932-2; Q3LI76: KRTAP15-1; NbExp=3; IntAct=EBI-11959123, EBI-11992140;
CC Q99932-2; Q3LHN2: KRTAP19-2; NbExp=3; IntAct=EBI-11959123, EBI-12196745;
CC Q99932-2; Q3LI70: KRTAP19-6; NbExp=3; IntAct=EBI-11959123, EBI-12805508;
CC Q99932-2; Q3SYF9: KRTAP19-7; NbExp=3; IntAct=EBI-11959123, EBI-10241353;
CC Q99932-2; Q3LI59: KRTAP21-2; NbExp=3; IntAct=EBI-11959123, EBI-18395721;
CC Q99932-2; Q6PEX3: KRTAP26-1; NbExp=3; IntAct=EBI-11959123, EBI-3957672;
CC Q99932-2; Q9BYR8: KRTAP3-1; NbExp=3; IntAct=EBI-11959123, EBI-9996449;
CC Q99932-2; Q3LI66: KRTAP6-2; NbExp=5; IntAct=EBI-11959123, EBI-11962084;
CC Q99932-2; Q8IUC3: KRTAP7-1; NbExp=3; IntAct=EBI-11959123, EBI-18394498;
CC Q99932-2; Q14847-2: LASP1; NbExp=3; IntAct=EBI-11959123, EBI-9088686;
CC Q99932-2; P50221: MEOX1; NbExp=3; IntAct=EBI-11959123, EBI-2864512;
CC Q99932-2; A6NJ78-4: METTL15; NbExp=3; IntAct=EBI-11959123, EBI-10174029;
CC Q99932-2; Q92597: NDRG1; NbExp=3; IntAct=EBI-11959123, EBI-716486;
CC Q99932-2; P49821: NDUFV1; NbExp=3; IntAct=EBI-11959123, EBI-748312;
CC Q99932-2; P07196: NEFL; NbExp=3; IntAct=EBI-11959123, EBI-475646;
CC Q99932-2; P0CG21: NHLRC4; NbExp=3; IntAct=EBI-11959123, EBI-12868744;
CC Q99932-2; O43809: NUDT21; NbExp=3; IntAct=EBI-11959123, EBI-355720;
CC Q99932-2; Q9HBE1-4: PATZ1; NbExp=3; IntAct=EBI-11959123, EBI-11022007;
CC Q99932-2; Q9UBV8: PEF1; NbExp=3; IntAct=EBI-11959123, EBI-724639;
CC Q99932-2; O00628-2: PEX7; NbExp=3; IntAct=EBI-11959123, EBI-25882083;
CC Q99932-2; Q99471: PFDN5; NbExp=3; IntAct=EBI-11959123, EBI-357275;
CC Q99932-2; O43189: PHF1; NbExp=3; IntAct=EBI-11959123, EBI-530034;
CC Q99932-2; Q9BXM7: PINK1; NbExp=3; IntAct=EBI-11959123, EBI-2846068;
CC Q99932-2; P78337: PITX1; NbExp=4; IntAct=EBI-11959123, EBI-748265;
CC Q99932-2; O15496: PLA2G10; NbExp=3; IntAct=EBI-11959123, EBI-726466;
CC Q99932-2; P50897: PPT1; NbExp=3; IntAct=EBI-11959123, EBI-1237011;
CC Q99932-2; O60260-5: PRKN; NbExp=6; IntAct=EBI-11959123, EBI-21251460;
CC Q99932-2; P60891: PRPS1; NbExp=3; IntAct=EBI-11959123, EBI-749195;
CC Q99932-2; P0CG20: PRR35; NbExp=3; IntAct=EBI-11959123, EBI-11986293;
CC Q99932-2; P49768-2: PSEN1; NbExp=6; IntAct=EBI-11959123, EBI-11047108;
CC Q99932-2; P10745: RBP3; NbExp=3; IntAct=EBI-11959123, EBI-12806054;
CC Q99932-2; P57078-2: RIPK4; NbExp=3; IntAct=EBI-11959123, EBI-12854608;
CC Q99932-2; Q9Y3C5: RNF11; NbExp=3; IntAct=EBI-11959123, EBI-396669;
CC Q99932-2; Q9BVN2: RUSC1; NbExp=3; IntAct=EBI-11959123, EBI-6257312;
CC Q99932-2; Q9UGK8: SERGEF; NbExp=3; IntAct=EBI-11959123, EBI-465368;
CC Q99932-2; Q53HV7-2: SMUG1; NbExp=3; IntAct=EBI-11959123, EBI-12275818;
CC Q99932-2; Q8NB12: SMYD1; NbExp=3; IntAct=EBI-11959123, EBI-8463848;
CC Q99932-2; P37840: SNCA; NbExp=3; IntAct=EBI-11959123, EBI-985879;
CC Q99932-2; P14678-2: SNRPB; NbExp=3; IntAct=EBI-11959123, EBI-372475;
CC Q99932-2; P09234: SNRPC; NbExp=3; IntAct=EBI-11959123, EBI-766589;
CC Q99932-2; Q7Z699: SPRED1; NbExp=3; IntAct=EBI-11959123, EBI-5235340;
CC Q99932-2; Q96PV0: SYNGAP1; NbExp=3; IntAct=EBI-11959123, EBI-2682386;
CC Q99932-2; Q13148: TARDBP; NbExp=6; IntAct=EBI-11959123, EBI-372899;
CC Q99932-2; Q9BXF9: TEKT3; NbExp=4; IntAct=EBI-11959123, EBI-8644516;
CC Q99932-2; Q96M29: TEKT5; NbExp=3; IntAct=EBI-11959123, EBI-10239812;
CC Q99932-2; Q96A09: TENT5B; NbExp=3; IntAct=EBI-11959123, EBI-752030;
CC Q99932-2; Q96RU7: TRIB3; NbExp=3; IntAct=EBI-11959123, EBI-492476;
CC Q99932-2; Q8IWZ5: TRIM42; NbExp=5; IntAct=EBI-11959123, EBI-5235829;
CC Q99932-2; Q86WV8: TSC1; NbExp=6; IntAct=EBI-11959123, EBI-12806590;
CC Q99932-2; Q5W5X9-3: TTC23; NbExp=3; IntAct=EBI-11959123, EBI-9090990;
CC Q99932-2; Q5T6F2: UBAP2; NbExp=3; IntAct=EBI-11959123, EBI-2514383;
CC Q99932-2; Q9UMX0: UBQLN1; NbExp=3; IntAct=EBI-11959123, EBI-741480;
CC Q99932-2; O75604: USP2; NbExp=3; IntAct=EBI-11959123, EBI-743272;
CC Q99932-2; A5D8V6: VPS37C; NbExp=3; IntAct=EBI-11959123, EBI-2559305;
CC Q99932-2; O76024: WFS1; NbExp=3; IntAct=EBI-11959123, EBI-720609;
CC Q99932-2; P54577: YARS1; NbExp=3; IntAct=EBI-11959123, EBI-1048893;
CC Q99932-2; A0A0C4DGF1: ZBTB32; NbExp=7; IntAct=EBI-11959123, EBI-10188476;
CC Q99932-2; Q15915: ZIC1; NbExp=5; IntAct=EBI-11959123, EBI-11963196;
CC Q99932-2; Q9P0T4: ZNF581; NbExp=3; IntAct=EBI-11959123, EBI-745520;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q3V0Q6}. Nucleus
CC {ECO:0000250|UniProtKB:Q3V0Q6}. Cytoplasmic vesicle, secretory vesicle,
CC acrosome {ECO:0000269|PubMed:15014887, ECO:0000269|PubMed:17187156,
CC ECO:0000269|PubMed:8788182}. Cytoplasm, cytoskeleton, microtubule
CC organizing center {ECO:0000269|PubMed:19548270}. Cytoplasm,
CC cytoskeleton, spindle {ECO:0000269|PubMed:19548270}. Cytoplasm,
CC cytoskeleton, cilium axoneme {ECO:0000250|UniProtKB:E1BNS6}. Note=In
CC mature sperm cells, detected in the acrosomal region of the head and in
CC the middle piece of the tail (By similarity). Localized to the nucleus
CC and cytoplasm of spermatocytes and round spermatids while, in
CC elongating spermatids, expressed in the cytoplasm but not in the
CC nucleus (By similarity). During the cell cycle, localized on the
CC microtubule-organizing center (MTOC) during prophase. In metaphase,
CC extends along spindle microtubules. In anaphase, detected on the astral
CC microtubules and mid-zone. In telophase, remains at the mid-zone. After
CC cytokinesis, returns to the MTOC (PubMed:19548270). Microtubule inner
CC protein (MIP) part of the dynein-decorated doublet microtubules (DMTs)
CC in cilia axoneme (By similarity). {ECO:0000250|UniProtKB:E1BNS6,
CC ECO:0000250|UniProtKB:Q3V0Q6, ECO:0000269|PubMed:19548270}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=3;
CC IsoId=Q99932-3; Sequence=Displayed;
CC Name=1;
CC IsoId=Q99932-1; Sequence=VSP_059991, VSP_059992;
CC Name=2;
CC IsoId=Q99932-2; Sequence=VSP_059990;
CC -!- TISSUE SPECIFICITY: Expressed in testis (germ cells), but not in liver,
CC kidney, prostate and small intestine. {ECO:0000269|PubMed:8788182}.
CC -!- MISCELLANEOUS: [Isoform 1]: May be produced at very low levels due to a
CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC decay. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the SPAG8 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB46833.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR EMBL; S83157; AAB46833.1; ALT_SEQ; mRNA.
DR EMBL; U12978; AAA21129.2; -; mRNA.
DR EMBL; AK302313; BAG63651.1; -; mRNA.
DR EMBL; AL133410; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC019247; AAH19247.1; -; mRNA.
DR CCDS; CCDS43798.1; -. [Q99932-3]
DR CCDS; CCDS6592.1; -. [Q99932-2]
DR RefSeq; NP_001034681.1; NM_001039592.1. [Q99932-3]
DR RefSeq; NP_758516.1; NM_172312.1. [Q99932-2]
DR AlphaFoldDB; Q99932; -.
DR SMR; Q99932; -.
DR BioGRID; 117606; 89.
DR IntAct; Q99932; 113.
DR MINT; Q99932; -.
DR STRING; 9606.ENSP00000340982; -.
DR iPTMnet; Q99932; -.
DR PhosphoSitePlus; Q99932; -.
DR BioMuta; SPAG8; -.
DR DMDM; 62511128; -.
DR jPOST; Q99932; -.
DR MassIVE; Q99932; -.
DR PaxDb; Q99932; -.
DR PeptideAtlas; Q99932; -.
DR PRIDE; Q99932; -.
DR ProteomicsDB; 19756; -.
DR ProteomicsDB; 78524; -. [Q99932-1]
DR ProteomicsDB; 78525; -. [Q99932-2]
DR Antibodypedia; 26087; 224 antibodies from 26 providers.
DR DNASU; 26206; -.
DR Ensembl; ENST00000340291.6; ENSP00000340982.2; ENSG00000137098.14. [Q99932-2]
DR Ensembl; ENST00000396638.7; ENSP00000379878.2; ENSG00000137098.14. [Q99932-3]
DR Ensembl; ENST00000475644.5; ENSP00000418530.1; ENSG00000137098.14. [Q99932-1]
DR GeneID; 26206; -.
DR KEGG; hsa:26206; -.
DR MANE-Select; ENST00000396638.7; ENSP00000379878.2; NM_001039592.2; NP_001034681.1.
DR UCSC; uc003zye.4; human. [Q99932-3]
DR CTD; 26206; -.
DR DisGeNET; 26206; -.
DR GeneCards; SPAG8; -.
DR HGNC; HGNC:14105; SPAG8.
DR HPA; ENSG00000137098; Tissue enhanced (choroid plexus, fallopian tube, testis).
DR MalaCards; SPAG8; -.
DR MIM; 605731; gene.
DR neXtProt; NX_Q99932; -.
DR OpenTargets; ENSG00000137098; -.
DR PharmGKB; PA37843; -.
DR VEuPathDB; HostDB:ENSG00000137098; -.
DR eggNOG; ENOG502S06E; Eukaryota.
DR GeneTree; ENSGT00640000091617; -.
DR HOGENOM; CLU_052284_0_0_1; -.
DR InParanoid; Q99932; -.
DR OMA; HCLLYNW; -.
DR OrthoDB; 1153567at2759; -.
DR PhylomeDB; Q99932; -.
DR TreeFam; TF329075; -.
DR PathwayCommons; Q99932; -.
DR SignaLink; Q99932; -.
DR BioGRID-ORCS; 26206; 6 hits in 1062 CRISPR screens.
DR ChiTaRS; SPAG8; human.
DR GeneWiki; SPAG8; -.
DR GenomeRNAi; 26206; -.
DR Pharos; Q99932; Tbio.
DR PRO; PR:Q99932; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; Q99932; protein.
DR Bgee; ENSG00000137098; Expressed in right uterine tube and 171 other tissues.
DR ExpressionAtlas; Q99932; baseline and differential.
DR Genevisible; Q99932; HS.
DR GO; GO:0001669; C:acrosomal vesicle; IEA:UniProtKB-SubCell.
DR GO; GO:0005879; C:axonemal microtubule; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0001673; C:male germ cell nucleus; IEA:Ensembl.
DR GO; GO:0016020; C:membrane; NAS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:0008017; F:microtubule binding; IEA:InterPro.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0032092; P:positive regulation of protein binding; IEA:Ensembl.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007338; P:single fertilization; IEA:UniProtKB-KW.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR InterPro; IPR026124; Sperm-assoc_Ag8.
DR PANTHER; PTHR15510; PTHR15510; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell cycle; Cell projection; Cytoplasm;
KW Cytoplasmic vesicle; Cytoskeleton; Differentiation; Fertilization; Nucleus;
KW Reference proteome; Spermatogenesis.
FT CHAIN 1..485
FT /note="Sperm-associated antigen 8"
FT /id="PRO_0000072099"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 75..99
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 127..215
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 13..37
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 127..157
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 422..485
FT /note="GVSNIRTLDTPFRKNCSFSTPVPLSLGKLLPYEPENYPYQLGEISSLPCPGG
FT RLGGGGGRMTPF -> TWWPLPTQVPAAEDYLTWKEWGFTGVQEVLSALLRATPGEYSV
FT NICGMNEHPVCSRTWTNRLCHQEMGSKKTVTQEDRGW (in isoform 2)"
FT /id="VSP_059990"
FT VAR_SEQ 422..426
FT /note="GVSNI -> VCEGD (in isoform 1)"
FT /id="VSP_059991"
FT VAR_SEQ 427..485
FT /note="Missing (in isoform 1)"
FT /id="VSP_059992"
FT VARIANT 69
FT /note="A -> T (in dbSNP:rs13299596)"
FT /id="VAR_056992"
FT VARIANT 226
FT /note="P -> L (in dbSNP:rs17851728)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_069166"
FT CONFLICT 5
FT /note="E -> G (in Ref. 5; AAH19247)"
FT /evidence="ECO:0000305"
FT CONFLICT 48
FT /note="T -> A (in Ref. 3; BAG63651)"
FT /evidence="ECO:0000305"
FT CONFLICT 49
FT /note="A -> S (in Ref. 1; AAB46833)"
FT /evidence="ECO:0000305"
FT CONFLICT 176..179
FT /note="Missing (in Ref. 3; BAG63651)"
FT /evidence="ECO:0000305"
FT CONFLICT 269
FT /note="M -> Q (in Ref. 1; AAB46833)"
FT /evidence="ECO:0000305"
FT CONFLICT 322
FT /note="K -> T (in Ref. 1; AAB46833)"
FT /evidence="ECO:0000305"
FT CONFLICT 438
FT /note="S -> G (in Ref. 3; BAG63651)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 485 AA; 51139 MW; 206A2AE68C5A4887 CRC64;
METNESTEGS RSRSRSLDIQ PSSEGLGPTS EPFPSSDDSP RSALAAATAA AAAAASAAAA
TAAFTTAKAA ALSTKTPAPC SEFMEPSSDP SLLGEPCAGP GFTHNIAHGS LGFEPVYVSC
IAQDTCTTTD HSSNPGPVPG SSSGPVLGSS SGAGHGSGSG SGPGCGSVPG SGSGPGPGSG
PGSGPGHGSG SHPGPASGPG PDTGPDSELS PCIPPGFRNL VADRVPNYTS WSQHCPWEPQ
KQPPWEFLQV LEPGARGLWK PPDIKGKLMV CYETLPRGQC LLYNWEEERA TNHLDQVPSM
QDGSESFFFR HGHRGLLTMQ LKSPMPSSTT QKDSYQPPGN VYWPLRGKRE AMLEMLLQHQ
ICKEVQAEQE PTRKLFEVES VTHHDYRMEL AQAGTPAPTK PHDYRQEQPE TFWIQRAPQL
PGVSNIRTLD TPFRKNCSFS TPVPLSLGKL LPYEPENYPY QLGEISSLPC PGGRLGGGGG
RMTPF
