SPAK_SHIFL
ID SPAK_SHIFL Reviewed; 133 AA.
AC P35530;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 25-MAY-2022, entry version 128.
DE RecName: Full=Surface presentation of antigens protein SpaK;
DE AltName: Full=Class 1B type III secretion system chaperone spa15;
GN Name=spaK; Synonyms=spa15; OrderedLocusNames=CP0148;
OS Shigella flexneri.
OG Plasmid pWR100, Plasmid pMYSH6000, and Plasmid pCP301.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=1312536; DOI=10.1128/jb.174.6.1990-2001.1992;
RA Venkatesan M.M., Buysse J.M., Oaks E.V.;
RT "Surface presentation of Shigella flexneri invasion plasmid antigens
RT requires the products of the spa locus.";
RL J. Bacteriol. 174:1990-2001(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11115111; DOI=10.1046/j.1365-2958.2000.02179.x;
RA Buchrieser C., Glaser P., Rusniok C., Nedjari H., d'Hauteville H.,
RA Kunst F., Sansonetti P.J., Parsot C.;
RT "The virulence plasmid pWR100 and the repertoire of proteins secreted by
RT the type III secretion apparatus of Shigella flexneri.";
RL Mol. Microbiol. 38:760-771(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11292750; DOI=10.1128/iai.69.5.3271-3285.2001;
RA Venkatesan M.M., Goldberg M.B., Rose D.J., Grotbeck E.J., Burland V.,
RA Blattner F.R.;
RT "Complete DNA sequence and analysis of the large virulence plasmid of
RT Shigella flexneri.";
RL Infect. Immun. 69:3271-3285(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=YSH6000 / Serotype 2a; PLASMID=pMYSH6000;
RX PubMed=8385666; DOI=10.1128/jb.175.8.2334-2346.1993;
RA Sasakawa C., Komatsu K., Tobe T., Suzuki T., Yoshikawa M.;
RT "Eight genes in region 5 that form an operon are essential for invasion of
RT epithelial cells by Shigella flexneri 2a.";
RL J. Bacteriol. 175:2334-2346(1993).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a; PLASMID=pCP301;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (1.82 ANGSTROMS), SUBUNIT, AND FUNCTION.
RX PubMed=15088068; DOI=10.1038/sj.embor.7400144;
RA van Eerde A., Hamiaux C., Perez J., Parsot C., Dijkstra B.W.;
RT "Structure of Spa15, a type III secretion chaperone from Shigella flexneri
RT with broad specificity.";
RL EMBO Rep. 5:477-483(2004).
CC -!- FUNCTION: Required for surface presentation of invasion plasmid
CC antigens. Chaperone specialized in the storage of effectors within the
CC bacterial cytoplasm, maintaining them in a secretion-competent state,
CC and allowing their immediate delivery to target cells upon contact of
CC the bacterium with the host cells. Has been shown to chaperone IpaA,
CC IpgB1, OspC3 and probably also OspB. {ECO:0000269|PubMed:15088068}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:15088068}.
CC -!- SIMILARITY: Belongs to the SpaK family. {ECO:0000305}.
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DR EMBL; M81458; AAA26540.1; -; Genomic_DNA.
DR EMBL; AL391753; CAC05823.1; -; Genomic_DNA.
DR EMBL; AF348706; AAK18467.1; -; Genomic_DNA.
DR EMBL; D13663; BAA02824.1; -; Genomic_DNA.
DR EMBL; AF386526; AAL72308.1; -; Genomic_DNA.
DR PIR; B42284; B42284.
DR RefSeq; NP_085311.1; NC_002698.1.
DR RefSeq; NP_858281.1; NC_004851.1.
DR RefSeq; WP_000065502.1; NZ_WPGU01000437.1.
DR RefSeq; YP_009062505.1; NC_024996.1.
DR PDB; 1RY9; X-ray; 1.82 A; A/B/C/D=1-133.
DR PDB; 2XGA; X-ray; 2.30 A; A/B=1-133.
DR PDBsum; 1RY9; -.
DR PDBsum; 2XGA; -.
DR AlphaFoldDB; P35530; -.
DR SMR; P35530; -.
DR STRING; 198214.CP0148; -.
DR TCDB; 3.A.6.1.2; the type iii (virulence-related) secretory pathway (iiisp) family.
DR PRIDE; P35530; -.
DR EnsemblBacteria; AAL72308; AAL72308; SF_p0148.
DR GeneID; 1238004; -.
DR KEGG; sfl:CP0148; -.
DR HOGENOM; CLU_125441_0_0_6; -.
DR OMA; TIMEGCQ; -.
DR EvolutionaryTrace; P35530; -.
DR Proteomes; UP000001006; Plasmid pCP301.
DR InterPro; IPR003065; Invas_SpaK.
DR Pfam; PF03519; Invas_SpaK; 1.
DR PRINTS; PR01305; SSPAKPROTEIN.
PE 1: Evidence at protein level;
KW 3D-structure; Chaperone; Plasmid; Reference proteome; Virulence.
FT CHAIN 1..133
FT /note="Surface presentation of antigens protein SpaK"
FT /id="PRO_0000180966"
FT HELIX 4..17
FT /evidence="ECO:0007829|PDB:1RY9"
FT HELIX 21..23
FT /evidence="ECO:0007829|PDB:1RY9"
FT STRAND 24..28
FT /evidence="ECO:0007829|PDB:1RY9"
FT STRAND 33..37
FT /evidence="ECO:0007829|PDB:1RY9"
FT STRAND 40..48
FT /evidence="ECO:0007829|PDB:1RY9"
FT STRAND 51..59
FT /evidence="ECO:0007829|PDB:1RY9"
FT HELIX 63..77
FT /evidence="ECO:0007829|PDB:1RY9"
FT STRAND 90..93
FT /evidence="ECO:0007829|PDB:1RY9"
FT STRAND 95..104
FT /evidence="ECO:0007829|PDB:1RY9"
FT HELIX 106..108
FT /evidence="ECO:0007829|PDB:1RY9"
FT HELIX 112..132
FT /evidence="ECO:0007829|PDB:1RY9"
SQ SEQUENCE 133 AA; 15116 MW; EA8F145A85A002B1 CRC64;
MSNINLVQLV RDSLFTIGCP PSIITDLDSH SAITISLDSM PAINIALVNE QVMLWANFDA
PSDVKLQSSA YNILNLMLMN FSYSINELVE LHRSDEYLQL RVVIKDDYVH DGIVFAEILH
EFYQRMEILN GVL
