ID   SPAN1_BPMU              Reviewed;         128 AA.
AC   Q9T1X1;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   23-FEB-2022, entry version 47.
DE   RecName: Full=Probable spanin, inner membrane subunit;
DE            Short=i-spanin;
DE   AltName: Full=gene product 23;
DE            Short=gp23;
GN   OrderedLocusNames=Mup23;
OS   Escherichia phage Mu (Bacteriophage Mu).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Muvirus.
OX   NCBI_TaxID=10677;
OH   NCBI_TaxID=543; Enterobacteriaceae.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11922669; DOI=10.1006/jmbi.2002.5437;
RA   Morgan G.J., Hatfull G.F., Casjens S., Hendrix R.W.;
RT   "Bacteriophage Mu genome sequence: analysis and comparison with Mu-like
RT   prophages in Haemophilus, Neisseria and Deinococcus.";
RL   J. Mol. Biol. 317:337-359(2002).
RN   [2]
RP   IDENTIFICATION.
RX   PubMed=17900620; DOI=10.1016/j.jmb.2007.08.045;
RA   Summer E.J., Berry J., Tran T.A., Niu L., Struck D.K., Young R.;
RT   "Rz/Rz1 lysis gene equivalents in phages of Gram-negative hosts.";
RL   J. Mol. Biol. 373:1098-1112(2007).
RN   [3]
RP   INDUCTION.
RX   PubMed=2524470; DOI=10.1128/jb.171.6.3440-3448.1989;
RA   Stoddard S.F., Howe M.M.;
RT   "Localization and regulation of bacteriophage Mu promoters.";
RL   J. Bacteriol. 171:3440-3448(1989).
CC   -!- FUNCTION: Component of the spanin complex that disrupts the host outer
CC       membrane and causes cell lysis during virus exit. The spanin complex
CC       conducts the final step in host lysis by disrupting the outer membrane
CC       after holin and endolysin action have permeabilized the inner membrane
CC       and degraded the host peptidoglycans (Probable). {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Host cell inner membrane {ECO:0000305}.
CC   -!- INDUCTION: Expressed in the intermediate phase of the viral replicative
CC       cycle. {ECO:0000269|PubMed:2524470}.
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DR   EMBL; AF083977; AAF01101.1; -; Genomic_DNA.
DR   RefSeq; NP_050627.1; NC_000929.1.
DR   SMR; Q9T1X1; -.
DR   GeneID; 2636265; -.
DR   KEGG; vg:2636265; -.
DR   Proteomes; UP000002611; Genome.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Coiled coil; Cytolysis; Host cell inner membrane; Host cell lysis by virus;
KW   Host cell membrane; Host membrane; Membrane; Reference proteome;
KW   Viral release from host cell.
FT   CHAIN           1..128
FT                   /note="Probable spanin, inner membrane subunit"
FT                   /id="PRO_0000077816"
FT   COILED          24..51
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   128 AA;  14281 MW;  0D6613161EFD907D CRC64;
     MTFASKSLLL AAVFTAVLSG GLWHRLDSTR HDNQTLRREL QTEQQARHTA EWLLHGQEQT
     MQVFSAIRAA NRAARLADET EHHDAKEKIT TAITGDNCST RPVPAVAADR LRELEKRTRA
     IGGDPARN