ID   SPAN1_BPP2              Reviewed;         141 AA.
AC   P51770;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   23-FEB-2022, entry version 69.
DE   RecName: Full=Probable spanin, inner membrane subunit;
DE            Short=i-spanin;
DE   AltName: Full=Protein lysB;
GN   Name=lysB;
OS   Escherichia phage P2 (Bacteriophage P2).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Peduovirinae; Peduovirus.
OX   NCBI_TaxID=10679;
OH   NCBI_TaxID=543; Enterobacteriaceae.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8051010; DOI=10.1128/jb.176.16.4974-4984.1994;
RA   Ziermann R., Bartlett B., Calendar R., Christie G.E.;
RT   "Functions involved in bacteriophage P2-induced host cell lysis and
RT   identification of a new tail gene.";
RL   J. Bacteriol. 176:4974-4984(1994).
CC   -!- FUNCTION: Component of the spanin complex that disrupts the host outer
CC       membrane and participates in cell lysis during virus exit. The spanin
CC       complex conducts the final step in host lysis by disrupting the outer
CC       membrane after holin and endolysin action have permeabilized the inner
CC       membrane and degraded the host peptidoglycans. Host outer membrane
CC       disruption is possibly due to local fusion between the inner and outer
CC       membrane performed by the spanin complex (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts (via C-terminus) with the spanin outer lipoprotein
CC       subunit (via C-terminus). Part of the spanin complex which spans the
CC       entire periplasmic space. The spanin complex is composed of spanin
CC       inner membrane subunit and spanin outer membrane subunit (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cell inner membrane {ECO:0000250}; Single-
CC       pass type II membrane protein {ECO:0000250}; Periplasmic side
CC       {ECO:0000250}.
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DR   EMBL; AF063097; AAD03278.1; -; Genomic_DNA.
DR   PIR; F55855; F55855.
DR   RefSeq; NP_046767.1; NC_001895.1.
DR   SMR; P51770; -.
DR   GeneID; 1261521; -.
DR   KEGG; vg:1261521; -.
DR   Proteomes; UP000009092; Genome.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR020000; Phage_P2_LysB.
DR   TIGRFAMs; TIGR03495; phage_LysB; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytolysis; Host cell inner membrane; Host cell lysis by virus;
KW   Host cell membrane; Host membrane; Membrane; Reference proteome;
KW   Signal-anchor; Transmembrane; Transmembrane helix;
KW   Viral release from host cell.
FT   CHAIN           1..141
FT                   /note="Probable spanin, inner membrane subunit"
FT                   /id="PRO_0000165243"
FT   TRANSMEM        1..17
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        18..141
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          119..141
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   141 AA;  15811 MW;  8EFB62D29ABE116D CRC64;
     MSRLMIVLVV LLSLAVAGLF LVKHKNASLR ASLDRANNVA SGQQTTITML KNQLHVALTR
     ADKNELAQVA LRQELENAAK REAQREKTIT RLLNENEDFR RWYGADLPDA VRRLHQRPAC
     TDASDCPQRM PESEPLPDAG Q