ID   SPAN1_BPPA2             Reviewed;          64 AA.
AC   P10438;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Spanin, inner membrane subunit;
DE            Short=i-spanin;
DE   AltName: Full=Lysis protein;
DE   Flags: Fragment;
GN   Name=Rz;
OS   Enterobacteria phage PA-2 (Bacteriophage PA-2).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae; Lambdavirus; unclassified Lambdavirus.
OX   NCBI_TaxID=10738;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3017988; DOI=10.1016/s0021-9258(18)67152-2;
RA   Blasband A.J., Marcotte W.R. Jr., Schnaitman C.A.;
RT   "Structure of the lc and nmpC outer membrane porin protein genes of
RT   lambdoid bacteriophage.";
RL   J. Biol. Chem. 261:12723-12732(1986).
CC   -!- FUNCTION: Component of the spanin complex that disrupts the host outer
CC       membrane and causes cell lysis during virus exit. The spanin complex
CC       conducts the final step in host lysis by disrupting the outer membrane
CC       after holin and endolysin action have permeabilized the inner membrane
CC       and degraded the host peptidoglycans (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer; disulfide-linked. Interacts (via C-terminus) with
CC       RZ1 (via C-terminus). Part of the spanin complex which spans the entire
CC       periplasmic space. The spanin complex is composed of spanin, inner
CC       membrane subunit and spanin, outer membrane subunit (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cell inner membrane {ECO:0000250}; Single-
CC       pass type II membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the lambdalikevirus i-spanin family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; J02580; AAA32299.1; -; Genomic_DNA.
DR   SMR; P10438; -.
DR   MEROPS; X19.001; -.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Cytolysis; Disulfide bond; Host cell inner membrane;
KW   Host cell lysis by virus; Host cell membrane; Host membrane; Membrane;
KW   Signal-anchor; Transmembrane; Transmembrane helix;
KW   Viral release from host cell.
FT   CHAIN           1..>64
FT                   /note="Spanin, inner membrane subunit"
FT                   /id="PRO_0000077570"
FT   TOPO_DOM        1..3
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        4..24
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        25..>64
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   NON_TER         64
SQ   SEQUENCE   64 AA;  7201 MW;  A4A8B552308B5311 CRC64;
     MSRVTAIISA LIICIIISLS WAVNHYRDNA IAYKAQRDKN ARELKLANAA ITDMQMRQRD
     VAAL