ID   SPAN1_BPT3              Reviewed;         147 AA.
AC   P10305;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   07-APR-2021, entry version 67.
DE   RecName: Full=Spanin, inner membrane subunit {ECO:0000250|UniProtKB:P03803};
DE            Short=i-spanin;
DE   AltName: Full=Gene product 18.5;
DE            Short=Gp18.5;
GN   Name=18.5;
OS   Enterobacteria phage T3 (Bacteriophage T3).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Autographiviridae; Studiervirinae; Teetrevirus;
OC   Escherichia virus T3.
OX   NCBI_TaxID=10759;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3010556; DOI=10.1016/0042-6822(86)90055-3;
RA   Yamada M., Fujisawa H., Kato H., Hamada K., Minagawa T.;
RT   "Cloning and sequencing of the genetic right end of bacteriophage T3 DNA.";
RL   Virology 151:350-361(1986).
RN   [2]
RP   ERRATUM OF PUBMED:3010556.
RA   Yamada M., Fujisawa H., Kato H., Hamada K., Minagawa T.;
RL   Virology 154:246-246(1986).
CC   -!- FUNCTION: Component of the spanin complex that disrupts the host outer
CC       membrane and participates in cell lysis during virus exit. The spanin
CC       complex conducts the final step in host lysis by disrupting the outer
CC       membrane after holin and endolysin action have permeabilized the inner
CC       membrane and degraded the host peptidoglycans. Host outer membrane
CC       disruption is possibly due to local fusion between the inner and outer
CC       membrane performed by the spanin complex.
CC       {ECO:0000250|UniProtKB:P03803}.
CC   -!- SUBUNIT: Interacts (via C-terminus) with the spanin outer lipoprotein
CC       subunit (o-spanin) (via C-terminus). Part of the spanin complex which
CC       spans the entire periplasmic space. The spanin complex is composed of
CC       spanin inner membrane subunit and spanin outer membrane subunit.
CC       {ECO:0000250|UniProtKB:P03803}.
CC   -!- SUBCELLULAR LOCATION: Host cell inner membrane
CC       {ECO:0000250|UniProtKB:P03803}; Single-pass type II membrane protein
CC       {ECO:0000250|UniProtKB:P03803}; Periplasmic side
CC       {ECO:0000250|UniProtKB:P03803}.
CC   -!- SIMILARITY: Belongs to the T7likevirus i-spanin family. {ECO:0000305}.
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DR   EMBL; M14784; AAA92526.1; -; Genomic_DNA.
DR   PIR; D23476; Q8BPT3.
DR   SMR; P10305; -.
DR   MEROPS; X19.002; -.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0019076; P:viral release from host cell; IEA:InterPro.
DR   InterPro; IPR004929; I-spanin.
DR   InterPro; IPR016417; I-spanin_T7likevirus.
DR   Pfam; PF03245; Phage_lysis; 1.
DR   PIRSF; PIRSF004485; T7_18-5_prd; 1.
PE   3: Inferred from homology;
KW   Cytolysis; Host cell inner membrane; Host cell lysis by virus;
KW   Host cell membrane; Host membrane; Membrane; Signal-anchor; Transmembrane;
KW   Transmembrane helix; Viral release from host cell.
FT   CHAIN           1..147
FT                   /note="Spanin, inner membrane subunit"
FT                   /id="PRO_0000106536"
FT   TOPO_DOM        1..7
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P03803"
FT   TRANSMEM        8..24
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        25..143
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:P03803"
SQ   SEQUENCE   147 AA;  16911 MW;  B6FDD684ADF3FFD8 CRC64;
     MLEFLRKLIP WVLAGMLFGL GWHLGSDSMD AKWKQEVHNE YVKRVEATAS TQRAINEISA
     KYQEDLAALE GSTDRIISDL RQDNKRLRVR VKPTGTSEGQ CGFEPDDRAE LDDRDAKRIL
     SVTQKGDAWI RALQDTIREL QRKQEIK