ID   SPAN2_BP933             Reviewed;          61 AA.
AC   Q9XJJ7;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   02-DEC-2020, entry version 83.
DE   RecName: Full=Spanin, outer membrane subunit;
DE            Short=o-spanin;
DE   AltName: Full=Outer membrane lipoprotein Rz1;
DE   Flags: Precursor;
GN   Name=Rz1; OrderedLocusNames=L109;
OS   Escherichia phage 933W (Bacteriophage 933W).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Podoviridae; Sepvirinae; Traversvirus.
OX   NCBI_TaxID=10730;
OH   NCBI_TaxID=83334; Escherichia coli O157:H7.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=10074068; DOI=10.1128/jb.181.6.1767-1778.1999;
RA   Plunkett G. III, Rose D.J., Durfee T.J., Blattner F.R.;
RT   "Sequence of Shiga toxin 2 phage 933W from Escherichia coli O157:H7: Shiga
RT   toxin as a phage late-gene product.";
RL   J. Bacteriol. 181:1767-1778(1999).
CC   -!- FUNCTION: Component of the spanin complex that disrupts the host outer
CC       membrane and causes cell lysis during virus exit. The spanin complex
CC       conducts the final step in host lysis by disrupting the outer membrane
CC       after holin and endolysin action have permeabilized the inner membrane
CC       and degraded the host peptidoglycans (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer; disulfide-linked. Interacts (via C-terminus) with
CC       RZ (via C-terminus). Part of the spanin complex which spans the entire
CC       periplasmic space. The spanin complex is composed of spanin, inner
CC       membrane subunit and spanin, outer membrane subunit (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cell outer membrane {ECO:0000250}; Lipid-
CC       anchor {ECO:0000250}; Periplasmic side {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the lambdalikevirus o-spanin family.
CC       {ECO:0000305}.
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DR   EMBL; AF125520; AAD25453.1; -; Genomic_DNA.
DR   RefSeq; NP_049508.1; NC_000924.1.
DR   GeneID; 1261962; -.
DR   KEGG; vg:1261962; -.
DR   Proteomes; UP000002135; Genome.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0019076; P:viral release from host cell; IEA:InterPro.
DR   InterPro; IPR010346; O-spanin.
DR   Pfam; PF06085; Rz1; 1.
PE   3: Inferred from homology;
KW   Cytolysis; Disulfide bond; Host cell lysis by virus;
KW   Host cell outer membrane; Host membrane; Lipoprotein; Membrane; Palmitate;
KW   Reference proteome; Signal; Viral release from host cell.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000250"
FT   CHAIN           20..61
FT                   /note="Spanin, outer membrane subunit"
FT                   /id="PRO_0000003364"
FT   LIPID           20
FT                   /note="N-palmitoyl cysteine; by host"
FT                   /evidence="ECO:0000250"
FT   LIPID           20
FT                   /note="S-diacylglycerol cysteine; by host"
FT                   /evidence="ECO:0000250"
FT   DISULFID        30
FT                   /note="Interchain"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   61 AA;  6509 MW;  FAE16F60A615CC27 CRC64;
     MRELKMKLCV LMLPLVVSAC GSTPPAPVPC VKPPAPPAWI MQPAPDWQTP LNGIISSSER
     G