ID   SPAN2_BPKMV             Reviewed;          89 AA.
AC   P0DJZ3;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   14-MAY-2014, sequence version 1.
DT   02-JUN-2021, entry version 16.
DE   RecName: Full=Probable spanin, outer lipoprotein subunit;
DE   Flags: Precursor;
GN   ORFNames=46.1;
OS   Pseudomonas phage phiKMV.
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Autographiviridae; Krylovirinae; Phikmvvirus.
OX   NCBI_TaxID=204270;
OH   NCBI_TaxID=287; Pseudomonas aeruginosa.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=12890620; DOI=10.1016/s0042-6822(03)00123-5;
RA   Lavigne R., Burkal'tseva M.V., Robben J., Sykilinda N.N., Kurochkina L.P.,
RA   Grymonprez B., Jonckx B., Krylov V.N., Mesyanzhinov V.V., Volckaert G.;
RT   "The genome of bacteriophage phiKMV, a T7-like virus infecting Pseudomonas
RT   aeruginosa.";
RL   Virology 312:49-59(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15549178; DOI=10.1007/s00018-004-4301-y;
RA   Lavigne R., Briers Y., Hertveldt K., Robben J., Volckaert G.;
RT   "Identification and characterization of a highly thermostable bacteriophage
RT   lysozyme.";
RL   Cell. Mol. Life Sci. 61:2753-2759(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16522177; DOI=10.2174/0929866054696127;
RA   Lavigne R., Roucourt B., Hertveldt K., Volckaert G.;
RT   "Characterization of the bacteriophage PhiKMV DNA ligase.";
RL   Protein Pept. Lett. 12:645-648(2005).
CC   -!- FUNCTION: Component of the spanin complex that disrupts the host outer
CC       membrane and participates in cell lysis during virus exit. The spanin
CC       complex conducts the final step in host lysis by disrupting the outer
CC       membrane after holin and endolysin action have permeabilized the inner
CC       membrane and degraded the host peptidoglycans. Host outer membrane
CC       disruption is possibly due to local fusion between the inner and outer
CC       membrane performed by the spanin complex (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts (via C-terminus) with the spanin inner membrane
CC       subunit (via C-terminus). Part of the spanin complex which spans the
CC       entire periplasmic space. The spanin complex is composed of spanin
CC       inner membrane subunit and spanin outer membrane subunit (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cell outer membrane {ECO:0000250}; Lipid-
CC       anchor {ECO:0000250}; Periplasmic side {ECO:0000250}.
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DR   EMBL; AJ505558; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   TCDB; 1.M.1.3.1; the rz/rz1 spanin1 (rz(1)) family.
DR   Proteomes; UP000000842; Genome.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Cytolysis; Host cell lysis by virus; Host cell outer membrane;
KW   Host membrane; Lipoprotein; Membrane; Reference proteome; Signal;
KW   Viral release from host cell.
FT   SIGNAL          1..47
FT                   /evidence="ECO:0000255"
FT   CHAIN           48..89
FT                   /note="Probable spanin, outer lipoprotein subunit"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000429262"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   89 AA;  9326 MW;  E744E2F1D7FD1540 CRC64;
     MPPCSATSRR SLPGPTPSAR RWTVPWTRTA RGLTGLCLLL SLTACATAPA PAVLCEHPLI
     DPTTQAGLIR AVAAYQDALD LCNALNQGD