ID   SPAN2_BPP2              Reviewed;          96 AA.
AC   Q8LTD0;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 49.
DE   RecName: Full=Probable spanin, outer lipoprotein subunit;
DE   AltName: Full=Protein lysC;
DE   Flags: Precursor;
GN   Name=lysC;
OS   Escherichia phage P2 (Bacteriophage P2).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Peduovirinae; Peduovirus.
OX   NCBI_TaxID=10679;
OH   NCBI_TaxID=543; Enterobacteriaceae.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Christie G.E., Haggard-Ljungquist E., Calendar R.;
RT   "The complete genome of bacteriophage P2.";
RL   Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the spanin complex that disrupts the host outer
CC       membrane and participates in cell lysis during virus exit. The spanin
CC       complex conducts the final step in host lysis by disrupting the outer
CC       membrane after holin and endolysin action have permeabilized the inner
CC       membrane and degraded the host peptidoglycans. Host outer membrane
CC       disruption is possibly due to local fusion between the inner and outer
CC       membrane performed by the spanin complex (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts (via C-terminus) with the spanin inner membrane
CC       subunit (via C-terminus). Part of the spanin complex which spans the
CC       entire periplasmic space. The spanin complex is composed of spanin
CC       inner membrane subunit and spanin outer membrane subunit (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cell outer membrane {ECO:0000250}; Lipid-
CC       anchor {ECO:0000250}; Periplasmic side {ECO:0000250}.
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DR   EMBL; AF063097; AAM83596.1; -; Genomic_DNA.
DR   RefSeq; NP_757382.1; NC_001895.1.
DR   PRIDE; Q8LTD0; -.
DR   GeneID; 1261522; -.
DR   KEGG; vg:1261522; -.
DR   Proteomes; UP000009092; Genome.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Coiled coil; Cytolysis; Host cell lysis by virus; Host cell outer membrane;
KW   Host membrane; Lipoprotein; Membrane; Reference proteome; Signal;
KW   Viral release from host cell.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..96
FT                   /note="Probable spanin, outer lipoprotein subunit"
FT                   /id="PRO_0000429263"
FT   TOPO_DOM        25..96
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   COILED          54..78
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   96 AA;  10053 MW;  DF8B0B052486B95E CRC64;
     MRTKIFAAGT VLTCLMLCAG CTSAPPAPTP VIVPNACPKV SLCPMPGSDP QTNGDLSADI
     RQLENALARC ASQVKMIKHC QDENDAQTRQ PAQGAD