ID   SPAN2_BPP22             Reviewed;          61 AA.
AC   A8CGF6; Q8LTE9;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   12-AUG-2020, entry version 46.
DE   RecName: Full=Probable spanin, outer lipoprotein subunit;
DE            Short=o-spanin;
DE   AltName: Full=Rz1 protein;
DE   Flags: Precursor;
GN   Name=Rz1;
OS   Salmonella phage P22 (Bacteriophage P22).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Podoviridae; Lederbergvirus.
OX   NCBI_TaxID=10754;
OH   NCBI_TaxID=90371; Salmonella typhimurium.
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=2998005; DOI=10.1016/0042-6822(85)90115-1;
RA   Rennell D., Poteete A.R.;
RT   "Phage P22 lysis genes: nucleotide sequences and functional relationships
RT   with T4 and lambda genes.";
RL   Virology 143:280-289(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2763468; DOI=10.1016/0042-6822(89)90628-4;
RA   Casjens S., Eppler K., Parr R., Poteete A.R.;
RT   "Nucleotide sequence of the bacteriophage P22 gene 19 to 3 region:
RT   identification of a new gene required for lysis.";
RL   Virology 171:588-598(1989).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Casjens S.R., Pedulla M., Hendrix R.W., Poteete A.R., Hatfull G.F.,
RA   Ford M.E., Karthikeyan T., Houtz J.M., Winn D.A.;
RL   Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Wild type;
RX   PubMed=12562822; DOI=10.1128/jb.185.4.1475-1477.2003;
RA   Pedulla M.L., Ford M.E., Karthikeyan T., Houtz J.M., Hendrix R.W.,
RA   Hatfull G.F., Poteete A.R., Gilcrease E.B., Winn-Stapley D.A.,
RA   Casjens S.R.;
RT   "Corrected sequence of the bacteriophage P22 genome.";
RL   J. Bacteriol. 185:1475-1477(2003).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 19585-B1, and MSU;
RX   PubMed=18621868; DOI=10.1128/aem.00352-08;
RA   Masago Y., Shibata T., Rose J.B.;
RT   "Bacteriophage P22 and Staphylococcus aureus attenuation on nonporous
RT   fomites as determined by plate assay and quantitative PCR.";
RL   Appl. Environ. Microbiol. 74:5838-5840(2008).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 19585-B1;
RA   Masago Y., Fong T.T., Rose J.B.;
RT   "The molecular identity of contaminant hydrology (20 years later).";
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the spanin complex that disrupts the host outer
CC       membrane and participates in cell lysis during virus exit. The spanin
CC       complex conducts the final step in host lysis by disrupting the outer
CC       membrane after holin and endolysin action have permeabilized the inner
CC       membrane and degraded the host peptidoglycans. Host outer membrane
CC       disruption is possibly due to local fusion between the inner and outer
CC       membrane performed by the spanin complex (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts (via C-terminus) with the spanin inner membrane
CC       subunit (via C-terminus). Part of the spanin complex which spans the
CC       entire periplasmic space. The spanin complex is composed of spanin
CC       inner membrane subunit and spanin outer membrane subunit (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cell outer membrane {ECO:0000250}; Lipid-
CC       anchor {ECO:0000250}; Periplasmic side {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the caudovirales o-spanin family. {ECO:0000305}.
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DR   EMBL; BK000583; DAA01048.1; -; Genomic_DNA.
DR   EMBL; AF527608; AAM81450.1; -; Genomic_DNA.
DR   EMBL; AB362338; BAF80782.1; -; Genomic_DNA.
DR   EMBL; AB426868; BAG12665.1; -; Genomic_DNA.
DR   RefSeq; YP_063732.1; NC_002371.2.
DR   TCDB; 1.M.1.1.4; the rz/rz1 spanin1 (rz(1)) family.
DR   GeneID; 2944226; -.
DR   KEGG; vg:2944226; -.
DR   Proteomes; UP000001315; Genome.
DR   Proteomes; UP000001795; Genome.
DR   Proteomes; UP000001796; Genome.
DR   Proteomes; UP000002165; Genome.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0019076; P:viral release from host cell; IEA:InterPro.
DR   InterPro; IPR010346; O-spanin.
DR   Pfam; PF06085; Rz1; 1.
PE   3: Inferred from homology;
KW   Cytolysis; Host cell lysis by virus; Host cell outer membrane;
KW   Host membrane; Lipoprotein; Membrane; Reference proteome; Signal;
KW   Viral release from host cell.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..61
FT                   /note="Probable spanin, outer lipoprotein subunit"
FT                   /id="PRO_0000429261"
FT   TOPO_DOM        29..61
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   61 AA;  6617 MW;  2C30817CC6B76A7C CRC64;
     MLTRLSKLSA LMFLLGVSAC KSPPPVQSQR PEPAAWAMEK AQDLQQMLNS IITVSEVEST
     G