ID   SPAN2_BPT3              Reviewed;          83 AA.
AC   P10302;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   07-OCT-2020, entry version 43.
DE   RecName: Full=Spanin, outer lipoprotein subunit;
DE            Short=o-spanin;
DE   AltName: Full=Gene product 18.7;
DE            Short=Gp18.7;
DE   AltName: Full=Protein 18.7;
DE   Flags: Precursor;
GN   Name=18.7;
OS   Enterobacteria phage T3 (Bacteriophage T3).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Autographiviridae; Studiervirinae; Teetrevirus;
OC   Escherichia virus T3.
OX   NCBI_TaxID=10759;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3010556; DOI=10.1016/0042-6822(86)90055-3;
RA   Yamada M., Fujisawa H., Kato H., Hamada K., Minagawa T.;
RT   "Cloning and sequencing of the genetic right end of bacteriophage T3 DNA.";
RL   Virology 151:350-361(1986).
RN   [2]
RP   ERRATUM OF PUBMED:3010556.
RA   Yamada M., Fujisawa H., Kato H., Hamada K., Minagawa T.;
RL   Virology 154:246-246(1986).
CC   -!- FUNCTION: Component of the spanin complex that disrupts the host outer
CC       membrane and participates in cell lysis during virus exit. The spanin
CC       complex conducts the final step in host lysis by disrupting the outer
CC       membrane after holin and endolysin action have permeabilized the inner
CC       membrane and degraded the host peptidoglycans. Host outer membrane
CC       disruption is possibly due to local fusion between the inner and outer
CC       membrane performed by the spanin complex.
CC       {ECO:0000250|UniProtKB:P03788}.
CC   -!- SUBUNIT: Homodimer. Interacts (via C-terminus) with the spanin inner
CC       membrane subunit (via C-terminus). Part of the spanin complex which
CC       spans the entire periplasmic space. The spanin complex is composed of
CC       spanin inner membrane subunit and spanin outer membrane subunit.
CC       {ECO:0000250|UniProtKB:P03788}.
CC   -!- SUBCELLULAR LOCATION: Host cell outer membrane
CC       {ECO:0000250|UniProtKB:P03788}; Lipid-anchor
CC       {ECO:0000250|UniProtKB:P03788}; Periplasmic side
CC       {ECO:0000250|UniProtKB:P03788}.
CC   -!- SIMILARITY: Belongs to the T7likevirus o-spanin family. {ECO:0000305}.
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DR   EMBL; M14784; AAA92527.1; -; Genomic_DNA.
DR   PIR; E23476; W8BPT3.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0019076; P:viral release from host cell; IEA:InterPro.
DR   InterPro; IPR020130; O-spanin_T7likevirus.
DR   Pfam; PF17531; O_Spanin_T7; 1.
PE   3: Inferred from homology;
KW   Cytolysis; Host cell lysis by virus; Host cell outer membrane;
KW   Host membrane; Lipoprotein; Membrane; Signal; Viral release from host cell.
FT   SIGNAL          1..50
FT                   /evidence="ECO:0000255"
FT   CHAIN           51..83
FT                   /note="Spanin, outer lipoprotein subunit"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000106538"
SQ   SEQUENCE   83 AA;  9393 MW;  63128984CBAF0531 CRC64;
     MSTLRELRLR RALKEQSMKY RLSIKKTLPR WKGALIGLFL ICVRTISGSG SESNLPEPPK
     VSVDSSLMIE PNLTTEMLNV FSQ