ID   SPAN2_BPT4              Reviewed;          99 AA.
AC   P39503; Q9T0T4;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   15-AUG-2003, sequence version 3.
DT   12-AUG-2020, entry version 63.
DE   RecName: Full=Spanin, outer lipoprotein subunit;
DE            Short=o-spanin;
DE   Flags: Precursor;
GN   Name=y13J; Synonyms=pseT.2;
OS   Enterobacteria phage T4 (Bacteriophage T4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX   NCBI_TaxID=10665;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA   Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT   "Bacteriophage T4 genome.";
RL   Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN   [2]
RP   FUNCTION.
RX   PubMed=17900620; DOI=10.1016/j.jmb.2007.08.045;
RA   Summer E.J., Berry J., Tran T.A., Niu L., Struck D.K., Young R.;
RT   "Rz/Rz1 lysis gene equivalents in phages of Gram-negative hosts.";
RL   J. Mol. Biol. 373:1098-1112(2007).
CC   -!- FUNCTION: Component of the spanin complex that disrupts the host outer
CC       membrane and participates in cell lysis during virus exit. The spanin
CC       complex conducts the final step in host lysis by disrupting the outer
CC       membrane after holin and endolysin action have permeabilized the inner
CC       membrane and degraded the host peptidoglycans. Host outer membrane
CC       disruption is possibly due to local fusion between the inner and outer
CC       membrane performed by the spanin complex (By similarity). {ECO:0000250,
CC       ECO:0000269|PubMed:17900620}.
CC   -!- SUBUNIT: Interacts (via C-terminus) with the spanin inner membrane
CC       subunit (via C-terminus). Part of the spanin complex which spans the
CC       entire periplasmic space. The spanin complex is composed of spanin
CC       inner membrane subunit and spanin outer membrane subunit (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cell outer membrane {ECO:0000250}; Lipid-
CC       anchor {ECO:0000250}; Periplasmic side {ECO:0000250}.
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DR   EMBL; AF158101; AAD42644.2; -; Genomic_DNA.
DR   RefSeq; NP_049836.2; NC_000866.4.
DR   TCDB; 1.M.3.1.1; the spanin3 (spanin3) family.
DR   GeneID; 1258607; -.
DR   KEGG; vg:1258607; -.
DR   Proteomes; UP000009087; Genome.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0044659; P:viral release from host cell by cytolysis; IGI:CACAO.
PE   3: Inferred from homology;
KW   Cytolysis; Host cell lysis by virus; Host cell outer membrane;
KW   Host membrane; Lipoprotein; Membrane; Palmitate; Reference proteome;
KW   Signal; Viral release from host cell.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000255"
FT   CHAIN           17..99
FT                   /note="Spanin, outer lipoprotein subunit"
FT                   /id="PRO_0000165183"
FT   TOPO_DOM        18..99
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   LIPID           17
FT                   /note="N-palmitoyl cysteine; by host"
FT                   /evidence="ECO:0000250"
FT   LIPID           17
FT                   /note="S-diacylglycerol cysteine; by host"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   99 AA;  11628 MW;  4E710D17320DE34F CRC64;
     MIKLSAVILS IGLLVGCSTK PLEVKKETVH PNWPVQIKSY DEAKLSWQVK VIDGKAWVGM
     PFEDSQEFRI WLNDVKRYVH DQKTMXCYYR QELKEDKCK