ID   SPAN2_BPT7              Reviewed;          83 AA.
AC   P03788;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   23-FEB-2022, entry version 70.
DE   RecName: Full=Spanin, outer lipoprotein subunit {ECO:0000303|PubMed:17900620};
DE            Short=o-spanin;
DE   AltName: Full=Gene product 18.7;
DE            Short=Gp18.7;
DE   AltName: Full=Protein 18.7;
DE   Flags: Precursor;
GN   OrderedLocusNames=18.7;
OS   Escherichia phage T7 (Bacteriophage T7).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Autographiviridae; Studiervirinae; Teseptimavirus.
OX   NCBI_TaxID=10760;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=6864790; DOI=10.1016/s0022-2836(83)80282-4;
RA   Dunn J.J., Studier F.W.;
RT   "Complete nucleotide sequence of bacteriophage T7 DNA and the locations of
RT   T7 genetic elements.";
RL   J. Mol. Biol. 166:477-535(1983).
RN   [2]
RP   INTERACTION WITH I-SPANIN.
RX   PubMed=8528255; DOI=10.1038/ng0196-72;
RA   Bartel P.L., Roecklein J.A., SenGupta D., Fields S.;
RT   "A protein linkage map of Escherichia coli bacteriophage T7.";
RL   Nat. Genet. 12:72-77(1996).
RN   [3]
RP   IDENTIFICATION, AND FUNCTION.
RX   PubMed=17900620; DOI=10.1016/j.jmb.2007.08.045;
RA   Summer E.J., Berry J., Tran T.A., Niu L., Struck D.K., Young R.;
RT   "Rz/Rz1 lysis gene equivalents in phages of Gram-negative hosts.";
RL   J. Mol. Biol. 373:1098-1112(2007).
CC   -!- FUNCTION: Component of the spanin complex that disrupts the host outer
CC       membrane and participates in cell lysis during virus exit. The spanin
CC       complex conducts the final step in host lysis by disrupting the outer
CC       membrane after holin and endolysin action have permeabilized the inner
CC       membrane and degraded the host peptidoglycans. Host outer membrane
CC       disruption is possibly due to local fusion between the inner and outer
CC       membrane performed by the spanin complex.
CC       {ECO:0000305|PubMed:17900620}.
CC   -!- SUBUNIT: Homodimer. Interacts (via C-terminus) with the spanin inner
CC       membrane subunit (via C-terminus). Part of the spanin complex which
CC       spans the entire periplasmic space. The spanin complex is composed of
CC       spanin inner membrane subunit and spanin outer membrane subunit.
CC       {ECO:0000305|PubMed:8528255}.
CC   -!- SUBCELLULAR LOCATION: Host cell outer membrane
CC       {ECO:0000305|PubMed:17900620}; Lipid-anchor
CC       {ECO:0000305|PubMed:17900620}; Periplasmic side
CC       {ECO:0000305|PubMed:17900620}.
CC   -!- SIMILARITY: Belongs to the T7likevirus o-spanin family. {ECO:0000305}.
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DR   EMBL; V01146; CAA24439.1; -; Genomic_DNA.
DR   PIR; A04413; W8BPG7.
DR   RefSeq; NP_042009.1; NC_001604.1.
DR   IntAct; P03788; 1.
DR   MINT; P03788; -.
DR   GeneID; 1261057; -.
DR   KEGG; vg:1261057; -.
DR   Proteomes; UP000000840; Genome.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0019076; P:viral release from host cell; IEA:InterPro.
DR   InterPro; IPR020130; O-spanin_T7likevirus.
DR   Pfam; PF17531; O_Spanin_T7; 1.
PE   1: Evidence at protein level;
KW   Cytolysis; Host cell lysis by virus; Host cell outer membrane;
KW   Host membrane; Lipoprotein; Membrane; Reference proteome; Signal;
KW   Viral release from host cell.
FT   SIGNAL          1..50
FT                   /evidence="ECO:0000255"
FT   CHAIN           51..83
FT                   /note="Spanin, outer lipoprotein subunit"
FT                   /id="PRO_0000106539"
SQ   SEQUENCE   83 AA;  9326 MW;  B3DB055DE79A53C5 CRC64;
     MSTLRELRLR RALKEQSMRY LLSIKKTLPR WKGALIGLFL ICVATISGCA SESKLPEPPM
     VSVDSSLMVE PNLTTEMLNV FSQ