SPAN_SHIFL
ID SPAN_SHIFL Reviewed; 292 AA.
AC P0A1K5; P35532;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 99.
DE RecName: Full=Surface presentation of antigens protein SpaN;
DE AltName: Full=Spa32 protein;
GN Name=spaN; Synonyms=spa32; OrderedLocusNames=CP0151;
OS Shigella flexneri.
OG Plasmid pWR100, Plasmid pMYSH6000, Plasmid pINV_F6_M1382, and
OG Plasmid pCP301.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-8, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=1312536; DOI=10.1128/jb.174.6.1990-2001.1992;
RA Venkatesan M.M., Buysse J.M., Oaks E.V.;
RT "Surface presentation of Shigella flexneri invasion plasmid antigens
RT requires the products of the spa locus.";
RL J. Bacteriol. 174:1990-2001(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=YSH6000 / Serotype 2a; PLASMID=pMYSH6000;
RX PubMed=8385666; DOI=10.1128/jb.175.8.2334-2346.1993;
RA Sasakawa C., Komatsu K., Tobe T., Suzuki T., Yoshikawa M.;
RT "Eight genes in region 5 that form an operon are essential for invasion of
RT epithelial cells by Shigella flexneri 2a.";
RL J. Bacteriol. 175:2334-2346(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-8, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11115111; DOI=10.1046/j.1365-2958.2000.02179.x;
RA Buchrieser C., Glaser P., Rusniok C., Nedjari H., d'Hauteville H.,
RA Kunst F., Sansonetti P.J., Parsot C.;
RT "The virulence plasmid pWR100 and the repertoire of proteins secreted by
RT the type III secretion apparatus of Shigella flexneri.";
RL Mol. Microbiol. 38:760-771(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11292750; DOI=10.1128/iai.69.5.3271-3285.2001;
RA Venkatesan M.M., Goldberg M.B., Rose D.J., Grotbeck E.J., Burland V.,
RA Blattner F.R.;
RT "Complete DNA sequence and analysis of the large virulence plasmid of
RT Shigella flexneri.";
RL Infect. Immun. 69:3271-3285(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M1382 / Serotype 6; PLASMID=pINV_F6_M1382;
RX PubMed=14573649; DOI=10.1128/iai.71.11.6298-6306.2003;
RA Lan R., Stevenson G., Reeves P.R.;
RT "Comparison of two major forms of the Shigella virulence plasmid pINV:
RT positive selection is a major force driving the divergence.";
RL Infect. Immun. 71:6298-6306(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a; PLASMID=pCP301;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [7]
RP DISULFIDE BOND, AND MUTAGENESIS.
RX PubMed=7761426; DOI=10.1073/pnas.92.11.4927;
RA Watarai M., Tobe T., Yoshikawa M., Sasakawa C.;
RT "Disulfide oxidoreductase activity of Shigella flexneri is required for
RT release of Ipa proteins and invasion of epithelial cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:4927-4931(1995).
CC -!- FUNCTION: Required for surface presentation of invasion plasmid
CC antigens. Could play a role in preserving the translocation competence
CC of the ipa antigens. Required for invasion and for secretion of IpaB
CC and IpaD proteins.
CC -!- INTERACTION:
CC P0A1K5; Q6XVW5: spa33; NbExp=3; IntAct=EBI-971034, EBI-970890;
CC P0A1K5; Q6XVW1: spa40; NbExp=2; IntAct=EBI-971034, EBI-6405436;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11115111,
CC ECO:0000269|PubMed:1312536}. Cell outer membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}. Note=Secreted via the Mxi-
CC Spa type III secretion system (TTSS).
CC -!- SIMILARITY: Belongs to the SpaN family. {ECO:0000305}.
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DR EMBL; M81458; AAA26542.1; -; Genomic_DNA.
DR EMBL; D13663; BAA02827.1; -; Genomic_DNA.
DR EMBL; AL391753; CAC05826.1; -; Genomic_DNA.
DR EMBL; AF348706; AAK18470.1; -; Genomic_DNA.
DR EMBL; AY206439; AAP79014.1; -; Genomic_DNA.
DR EMBL; AF386526; AAL72305.1; -; Genomic_DNA.
DR PIR; D42284; D42284.
DR RefSeq; NP_085314.1; NC_002698.1.
DR RefSeq; NP_858284.1; NC_004851.1.
DR RefSeq; WP_001162455.1; NZ_WPGG01000042.1.
DR RefSeq; YP_009062508.1; NC_024996.1.
DR AlphaFoldDB; P0A1K5; -.
DR IntAct; P0A1K5; 3.
DR STRING; 198214.CP0151; -.
DR TCDB; 3.A.6.1.2; the type iii (virulence-related) secretory pathway (iiisp) family.
DR EnsemblBacteria; AAL72305; AAL72305; SF_p0151.
DR GeneID; 1238001; -.
DR KEGG; sfl:CP0151; -.
DR PATRIC; fig|198214.7.peg.5396; -.
DR HOGENOM; CLU_952818_0_0_6; -.
DR OMA; NESGNAD; -.
DR Proteomes; UP000001006; Plasmid pCP301.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE 1: Evidence at protein level;
KW Cell outer membrane; Direct protein sequencing; Disulfide bond; Membrane;
KW Plasmid; Reference proteome; Secreted; Virulence.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:11115111"
FT CHAIN 2..292
FT /note="Surface presentation of antigens protein SpaN"
FT /id="PRO_0000180939"
FT REGION 129..151
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 267..292
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 269..286
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 19..292
FT /evidence="ECO:0000250"
SQ SEQUENCE 292 AA; 32978 MW; 78A112B3D24AB63C CRC64;
MALDNINLNF SSDKQIEKCE KLSSIDNIDS LVLKKKRKVE IPEYSLIASN YFTIDKHFEH
KHDKGEIYSG IKNAFELRNE RATYSDIPES MAIKENILIP DQDIKAREKI NIGDMRGIFS
YNKSGNADKN FERSHTSSVN PDNLLESDNR NGQIGLKNHS LSIDKNIADI ISLLNGSVAK
SFELPVMNKN TADITPSMSL QEKSIVENDK NVFQKNSEMT YHFKQWGAGH SVSISVESGS
FVLKPSDQFV GNKLDLILKQ DAEGNYRFDS SQHNKGNKNN STGYNEQSEE EC
