SPAO_SHIFL
ID SPAO_SHIFL Reviewed; 293 AA.
AC P0A1K9; P35534; Q8VSG8;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 94.
DE RecName: Full=Surface presentation of antigens protein SpaO;
DE AltName: Full=Spa33 protein;
GN Name=spaO; Synonyms=spa33; OrderedLocusNames=CP0152;
OS Shigella flexneri.
OG Plasmid pWR100, Plasmid pMYSH6000, and Plasmid pCP301.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=1312536; DOI=10.1128/jb.174.6.1990-2001.1992;
RA Venkatesan M.M., Buysse J.M., Oaks E.V.;
RT "Surface presentation of Shigella flexneri invasion plasmid antigens
RT requires the products of the spa locus.";
RL J. Bacteriol. 174:1990-2001(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=YSH6000 / Serotype 2a; PLASMID=pMYSH6000;
RX PubMed=8385666; DOI=10.1128/jb.175.8.2334-2346.1993;
RA Sasakawa C., Komatsu K., Tobe T., Suzuki T., Yoshikawa M.;
RT "Eight genes in region 5 that form an operon are essential for invasion of
RT epithelial cells by Shigella flexneri 2a.";
RL J. Bacteriol. 175:2334-2346(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11115111; DOI=10.1046/j.1365-2958.2000.02179.x;
RA Buchrieser C., Glaser P., Rusniok C., Nedjari H., d'Hauteville H.,
RA Kunst F., Sansonetti P.J., Parsot C.;
RT "The virulence plasmid pWR100 and the repertoire of proteins secreted by
RT the type III secretion apparatus of Shigella flexneri.";
RL Mol. Microbiol. 38:760-771(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11292750; DOI=10.1128/iai.69.5.3271-3285.2001;
RA Venkatesan M.M., Goldberg M.B., Rose D.J., Grotbeck E.J., Burland V.,
RA Blattner F.R.;
RT "Complete DNA sequence and analysis of the large virulence plasmid of
RT Shigella flexneri.";
RL Infect. Immun. 69:3271-3285(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a; PLASMID=pCP301;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
CC -!- FUNCTION: Required for surface presentation of invasion plasmid
CC antigens. Could play a role in preserving the translocation competence
CC of the Ipa antigens. Required for invasion and for secretion of the
CC three Ipa proteins.
CC -!- SIMILARITY: Belongs to the FliN/MopA/SpaO family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL72304.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
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DR EMBL; M81458; AAA26543.1; -; Genomic_DNA.
DR EMBL; D13663; BAA02828.1; -; Genomic_DNA.
DR EMBL; AL391753; CAC05827.1; -; Genomic_DNA.
DR EMBL; AF348706; AAK18471.1; -; Genomic_DNA.
DR EMBL; AF386526; AAL72304.1; ALT_SEQ; Genomic_DNA.
DR PIR; F49846; F49846.
DR RefSeq; NP_085315.1; NC_002698.1.
DR RefSeq; NP_858285.1; NC_004851.1.
DR RefSeq; WP_000944309.1; NZ_WHSI01000037.1.
DR RefSeq; WP_000944310.1; NZ_WPGS01000043.1.
DR RefSeq; YP_009062509.1; NC_024996.1.
DR PDB; 4TT9; X-ray; 2.30 A; A/B/C/D=208-293.
DR PDBsum; 4TT9; -.
DR AlphaFoldDB; P0A1K9; -.
DR SMR; P0A1K9; -.
DR STRING; 198214.CP0152; -.
DR TCDB; 3.A.6.1.2; the type iii (virulence-related) secretory pathway (iiisp) family.
DR PRIDE; P0A1K9; -.
DR EnsemblBacteria; AAL72304; AAL72304; SF_p0152.
DR GeneID; 1238000; -.
DR KEGG; sfl:CP0152; -.
DR PATRIC; fig|198214.7.peg.5397; -.
DR HOGENOM; CLU_949621_0_0_6; -.
DR Proteomes; UP000001006; Plasmid pCP301.
DR GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR Gene3D; 2.30.330.10; -; 1.
DR InterPro; IPR001543; FliN-like_C.
DR InterPro; IPR036429; SpoA-like_sf.
DR InterPro; IPR003283; T3SS_OMP_SpaO.
DR Pfam; PF01052; FliMN_C; 1.
DR PRINTS; PR01339; TYPE3OMOPROT.
DR SUPFAM; SSF101801; SSF101801; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Plasmid; Reference proteome; Virulence.
FT CHAIN 1..293
FT /note="Surface presentation of antigens protein SpaO"
FT /id="PRO_0000184127"
FT VARIANT 13
FT /note="L -> I (in plasmid pCP301)"
FT VARIANT 143
FT /note="L -> S (in plasmid pMYSH6000 and plasmid pCP301)"
FT STRAND 224..238
FT /evidence="ECO:0007829|PDB:4TT9"
FT HELIX 239..247
FT /evidence="ECO:0007829|PDB:4TT9"
FT STRAND 250..253
FT /evidence="ECO:0007829|PDB:4TT9"
FT TURN 257..259
FT /evidence="ECO:0007829|PDB:4TT9"
FT STRAND 261..265
FT /evidence="ECO:0007829|PDB:4TT9"
FT STRAND 268..278
FT /evidence="ECO:0007829|PDB:4TT9"
FT STRAND 281..288
FT /evidence="ECO:0007829|PDB:4TT9"
SQ SEQUENCE 293 AA; 33418 MW; 5AC0BCDD96223E77 CRC64;
MLRIKHFDAN EKLQILYAKQ LCERFSIQTF KNKFTGSESL VTLTSVCGDW VIRIDTLSFL
KKKYEVFSGF STQESLLHLS KCVFIESSSV FSIPELSDKI TFRITNEIQY ATTGSHLCCF
SSSLGIIYFD KMPVLRNQVS LDLLHHLLEF CLGSSNVRLA TLKRIRTGDI IIVQKLYNLL
LCNQVIIGDY IVNDNNEAKI NLSESNGESE HTEVSLALFN YDDINVKVDF ILLEKNMTIN
ELKMYVENEL FKFPDDIVKH VNIKVNGSLV GHGELVSIED GYGIEISSWM VKE
