SPAP_SHIFL
ID SPAP_SHIFL Reviewed; 216 AA.
AC P0A1L3; P35529;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 102.
DE RecName: Full=Surface presentation of antigens protein SpaP;
DE AltName: Full=Spa24 protein;
GN Name=spaP; Synonyms=spa24; OrderedLocusNames=CP0153;
OS Shigella flexneri.
OG Plasmid pWR100, Plasmid pMYSH6000, and Plasmid pCP301.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=1312536; DOI=10.1128/jb.174.6.1990-2001.1992;
RA Venkatesan M.M., Buysse J.M., Oaks E.V.;
RT "Surface presentation of Shigella flexneri invasion plasmid antigens
RT requires the products of the spa locus.";
RL J. Bacteriol. 174:1990-2001(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=YSH6000 / Serotype 2a; PLASMID=pMYSH6000;
RX PubMed=8385666; DOI=10.1128/jb.175.8.2334-2346.1993;
RA Sasakawa C., Komatsu K., Tobe T., Suzuki T., Yoshikawa M.;
RT "Eight genes in region 5 that form an operon are essential for invasion of
RT epithelial cells by Shigella flexneri 2a.";
RL J. Bacteriol. 175:2334-2346(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11115111; DOI=10.1046/j.1365-2958.2000.02179.x;
RA Buchrieser C., Glaser P., Rusniok C., Nedjari H., d'Hauteville H.,
RA Kunst F., Sansonetti P.J., Parsot C.;
RT "The virulence plasmid pWR100 and the repertoire of proteins secreted by
RT the type III secretion apparatus of Shigella flexneri.";
RL Mol. Microbiol. 38:760-771(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11292750; DOI=10.1128/iai.69.5.3271-3285.2001;
RA Venkatesan M.M., Goldberg M.B., Rose D.J., Grotbeck E.J., Burland V.,
RA Blattner F.R.;
RT "Complete DNA sequence and analysis of the large virulence plasmid of
RT Shigella flexneri.";
RL Infect. Immun. 69:3271-3285(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a; PLASMID=pCP301;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
CC -!- FUNCTION: Required for surface presentation of invasion plasmid
CC antigens. Could play a role in preserving the translocation competence
CC of the ipa antigens. Required for invasion and for secretion of the
CC three Ipa proteins.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the FliP/MopC/SpaP family. {ECO:0000305}.
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DR EMBL; M81458; AAA26544.1; -; Genomic_DNA.
DR EMBL; D13663; BAA02829.1; -; Genomic_DNA.
DR EMBL; AL391753; CAC05828.1; -; Genomic_DNA.
DR EMBL; AF348706; AAK18472.1; -; Genomic_DNA.
DR EMBL; AF386526; AAL72307.1; -; Genomic_DNA.
DR PIR; F42284; F42284.
DR RefSeq; NP_085316.1; NC_002698.1.
DR RefSeq; NP_858286.1; NC_004851.1.
DR RefSeq; WP_000947539.1; NZ_WPGS01000043.1.
DR RefSeq; YP_009062510.1; NC_024996.1.
DR PDB; 6R6B; EM; 3.50 A; A/B/C/D/E=1-216.
DR PDB; 6RWY; EM; 5.11 A; a/b/c/d/e=1-216.
DR PDBsum; 6R6B; -.
DR PDBsum; 6RWY; -.
DR AlphaFoldDB; P0A1L3; -.
DR SMR; P0A1L3; -.
DR STRING; 198214.CP0153; -.
DR TCDB; 3.A.6.1.2; the type iii (virulence-related) secretory pathway (iiisp) family.
DR EnsemblBacteria; AAL72307; AAL72307; SF_p0153.
DR GeneID; 1238003; -.
DR KEGG; sfl:CP0153; -.
DR PATRIC; fig|198214.7.peg.5398; -.
DR HOGENOM; CLU_042028_2_0_6; -.
DR OMA; SIVLVMM; -.
DR Proteomes; UP000001006; Plasmid pCP301.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR InterPro; IPR005838; T3SS_IM_P.
DR InterPro; IPR005773; YopR.
DR PANTHER; PTHR30587; PTHR30587; 1.
DR Pfam; PF00813; FliP; 1.
DR PRINTS; PR01302; TYPE3IMPPROT.
DR TIGRFAMs; TIGR01102; yscR; 1.
DR PROSITE; PS01060; FLIP_1; 1.
DR PROSITE; PS01061; FLIP_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Membrane; Plasmid; Reference proteome;
KW Transmembrane; Transmembrane helix; Virulence.
FT CHAIN 1..216
FT /note="Surface presentation of antigens protein SpaP"
FT /id="PRO_0000191993"
FT TRANSMEM 8..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 52..72
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 183..203
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TURN 13..15
FT /evidence="ECO:0007829|PDB:6R6B"
FT HELIX 17..20
FT /evidence="ECO:0007829|PDB:6R6B"
FT STRAND 23..26
FT /evidence="ECO:0007829|PDB:6R6B"
FT HELIX 27..38
FT /evidence="ECO:0007829|PDB:6R6B"
FT TURN 39..42
FT /evidence="ECO:0007829|PDB:6R6B"
FT STRAND 44..47
FT /evidence="ECO:0007829|PDB:6R6B"
FT HELIX 49..77
FT /evidence="ECO:0007829|PDB:6R6B"
FT HELIX 85..94
FT /evidence="ECO:0007829|PDB:6R6B"
FT HELIX 99..106
FT /evidence="ECO:0007829|PDB:6R6B"
FT TURN 109..111
FT /evidence="ECO:0007829|PDB:6R6B"
FT HELIX 112..114
FT /evidence="ECO:0007829|PDB:6R6B"
FT TURN 136..138
FT /evidence="ECO:0007829|PDB:6R6B"
FT HELIX 139..158
FT /evidence="ECO:0007829|PDB:6R6B"
FT HELIX 159..161
FT /evidence="ECO:0007829|PDB:6R6B"
FT HELIX 163..173
FT /evidence="ECO:0007829|PDB:6R6B"
FT HELIX 182..195
FT /evidence="ECO:0007829|PDB:6R6B"
FT TURN 196..199
FT /evidence="ECO:0007829|PDB:6R6B"
FT HELIX 200..208
FT /evidence="ECO:0007829|PDB:6R6B"
FT TURN 209..211
FT /evidence="ECO:0007829|PDB:6R6B"
SQ SEQUENCE 216 AA; 24200 MW; 8BB50BB4CAEB69F8 CRC64;
MLSDMSLIAT LSFFTLLPFL VAAGTCYIKF SIVFVMVRNA LGLQQVPSNM TLNGIALIMA
LFVMKPIIEA GYENYLNGPQ KFDTISDIVR FSDSGLMEYK QYLKKHTDLE LARFFQRSEE
ENADLKSAEN NDYSLFSLLP AYALSEIKDA FKIGFYLYLP FVVVDLVISS ILLALGMMMM
SPITISVPIK LVLFVALDGW GILSKALIEQ YINIPA
