SPAQ_SHIFL
ID SPAQ_SHIFL Reviewed; 86 AA.
AC P0A1M4; P40705; Q55297;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=Surface presentation of antigens protein SpaQ;
DE AltName: Full=Protein spa9;
GN Name=spaQ; Synonyms=spa9; OrderedLocusNames=CP0154;
OS Shigella flexneri.
OG Plasmid pWR100, Plasmid pMYSH6000, and Plasmid pCP301.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=YSH6000 / Serotype 2a; PLASMID=pMYSH6000;
RX PubMed=8385666; DOI=10.1128/jb.175.8.2334-2346.1993;
RA Sasakawa C., Komatsu K., Tobe T., Suzuki T., Yoshikawa M.;
RT "Eight genes in region 5 that form an operon are essential for invasion of
RT epithelial cells by Shigella flexneri 2a.";
RL J. Bacteriol. 175:2334-2346(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11115111; DOI=10.1046/j.1365-2958.2000.02179.x;
RA Buchrieser C., Glaser P., Rusniok C., Nedjari H., d'Hauteville H.,
RA Kunst F., Sansonetti P.J., Parsot C.;
RT "The virulence plasmid pWR100 and the repertoire of proteins secreted by
RT the type III secretion apparatus of Shigella flexneri.";
RL Mol. Microbiol. 38:760-771(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11292750; DOI=10.1128/iai.69.5.3271-3285.2001;
RA Venkatesan M.M., Goldberg M.B., Rose D.J., Grotbeck E.J., Burland V.,
RA Blattner F.R.;
RT "Complete DNA sequence and analysis of the large virulence plasmid of
RT Shigella flexneri.";
RL Infect. Immun. 69:3271-3285(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a; PLASMID=pCP301;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-53.
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=1312536; DOI=10.1128/jb.174.6.1990-2001.1992;
RA Venkatesan M.M., Buysse J.M., Oaks E.V.;
RT "Surface presentation of Shigella flexneri invasion plasmid antigens
RT requires the products of the spa locus.";
RL J. Bacteriol. 174:1990-2001(1992).
CC -!- FUNCTION: Required for surface presentation of invasion plasmid
CC antigens. Could play a role in preserving the translocation competence
CC of the Ipa antigens. Required for invasion and for secretion of the
CC three Ipa proteins.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the FliQ/MopD/SpaQ family. {ECO:0000305}.
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DR EMBL; D13663; BAA02830.1; -; Genomic_DNA.
DR EMBL; AL391753; CAC05829.1; -; Genomic_DNA.
DR EMBL; AF348706; AAK18473.1; -; Genomic_DNA.
DR EMBL; AF386526; AAL72556.1; -; Genomic_DNA.
DR EMBL; M81458; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; H49846; H49846.
DR RefSeq; NP_085317.1; NC_002698.1.
DR RefSeq; NP_858287.1; NC_004851.1.
DR RefSeq; WP_001280545.1; NZ_UIQL01000031.1.
DR RefSeq; YP_009062511.1; NC_024996.1.
DR PDB; 6R6B; EM; 3.50 A; G/H/I/J=1-86.
DR PDB; 6RWY; EM; 5.11 A; g/h/i/j/k=1-86.
DR PDBsum; 6R6B; -.
DR PDBsum; 6RWY; -.
DR AlphaFoldDB; P0A1M4; -.
DR SMR; P0A1M4; -.
DR STRING; 198214.CP0154; -.
DR TCDB; 3.A.6.1.2; the type iii (virulence-related) secretory pathway (iiisp) family.
DR EnsemblBacteria; AAL72556; AAL72556; SF_p0154.
DR GeneID; 1238259; -.
DR KEGG; sfl:CP0154; -.
DR PATRIC; fig|198214.7.peg.5399; -.
DR HOGENOM; CLU_164516_1_2_6; -.
DR OMA; IKLLAVF; -.
DR Proteomes; UP000001006; Plasmid pCP301.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR InterPro; IPR002191; Bac_export_3.
DR InterPro; IPR006306; T3SS_HrpO.
DR PANTHER; PTHR34040; PTHR34040; 1.
DR Pfam; PF01313; Bac_export_3; 1.
DR PRINTS; PR00952; TYPE3IMQPROT.
DR TIGRFAMs; TIGR01403; fliQ_rel_III; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Membrane; Plasmid; Reference proteome;
KW Transmembrane; Transmembrane helix; Virulence.
FT CHAIN 1..86
FT /note="Surface presentation of antigens protein SpaQ"
FT /id="PRO_0000129110"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 54..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT HELIX 2..34
FT /evidence="ECO:0007829|PDB:6R6B"
FT HELIX 35..37
FT /evidence="ECO:0007829|PDB:6R6B"
FT STRAND 38..41
FT /evidence="ECO:0007829|PDB:6R6B"
FT STRAND 45..47
FT /evidence="ECO:0007829|PDB:6R6B"
FT HELIX 49..61
FT /evidence="ECO:0007829|PDB:6R6B"
FT HELIX 64..83
FT /evidence="ECO:0007829|PDB:6R6B"
SQ SEQUENCE 86 AA; 9429 MW; 552D3DBCB9C896E3 CRC64;
MSDIVYMGNK ALYLILIFSL WPVGIATVIG LSIGLLQTVT QLQEQTLPFG IKLIGVSISL
LLLSGWYGEV LLSFCHEIMF LIKSGV
