SPAS2_HUMAN
ID SPAS2_HUMAN Reviewed; 545 AA.
AC Q86XZ4; A8K8G9; Q9H7D4; Q9H8Y7; Q9H8Z8;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Spermatogenesis-associated serine-rich protein 2;
DE AltName: Full=Serine-rich spermatocytes and round spermatid 59 kDa protein;
DE AltName: Full=p59scr;
GN Name=SPATS2; Synonyms=SCR59, SPATA10; ORFNames=Nbla00526;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Neuroblastoma;
RX PubMed=12880961; DOI=10.1016/s0304-3835(03)00085-5;
RA Ohira M., Morohashi A., Nakamura Y., Isogai E., Furuya K., Hamano S.,
RA Machida T., Aoyama M., Fukumura M., Miyazaki K., Suzuki Y., Sugano S.,
RA Hirato J., Nakagawara A.;
RT "Neuroblastoma oligo-capping cDNA project: toward the understanding of the
RT genesis and biology of neuroblastoma.";
RL Cancer Lett. 197:63-68(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-520, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SPATS2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB14448.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAB14958.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB075515; BAE45760.1; -; mRNA.
DR EMBL; AK023179; BAB14448.1; ALT_INIT; mRNA.
DR EMBL; AK023202; BAB14460.1; -; mRNA.
DR EMBL; AK024678; BAB14958.1; ALT_INIT; mRNA.
DR EMBL; AK292334; BAF85023.1; -; mRNA.
DR EMBL; CH471111; EAW58068.1; -; Genomic_DNA.
DR EMBL; BC048299; AAH48299.1; -; mRNA.
DR CCDS; CCDS31794.1; -.
DR RefSeq; NP_001280214.1; NM_001293285.1.
DR RefSeq; NP_001280215.1; NM_001293286.1.
DR RefSeq; NP_075559.2; NM_023071.3.
DR RefSeq; XP_011536992.1; XM_011538690.2.
DR RefSeq; XP_011536993.1; XM_011538691.2.
DR AlphaFoldDB; Q86XZ4; -.
DR BioGRID; 122410; 142.
DR IntAct; Q86XZ4; 30.
DR MINT; Q86XZ4; -.
DR STRING; 9606.ENSP00000448228; -.
DR GlyGen; Q86XZ4; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q86XZ4; -.
DR MetOSite; Q86XZ4; -.
DR PhosphoSitePlus; Q86XZ4; -.
DR BioMuta; SPATS2; -.
DR DMDM; 74750549; -.
DR EPD; Q86XZ4; -.
DR jPOST; Q86XZ4; -.
DR MassIVE; Q86XZ4; -.
DR MaxQB; Q86XZ4; -.
DR PaxDb; Q86XZ4; -.
DR PeptideAtlas; Q86XZ4; -.
DR PRIDE; Q86XZ4; -.
DR ProteomicsDB; 70343; -.
DR Antibodypedia; 48974; 49 antibodies from 12 providers.
DR DNASU; 65244; -.
DR Ensembl; ENST00000321898.10; ENSP00000326841.6; ENSG00000123352.18.
DR Ensembl; ENST00000552918.6; ENSP00000447947.2; ENSG00000123352.18.
DR Ensembl; ENST00000553127.5; ENSP00000448228.1; ENSG00000123352.18.
DR GeneID; 65244; -.
DR KEGG; hsa:65244; -.
DR MANE-Select; ENST00000552918.6; ENSP00000447947.2; NM_023071.4; NP_075559.2.
DR UCSC; uc001rud.3; human.
DR CTD; 65244; -.
DR DisGeNET; 65244; -.
DR GeneCards; SPATS2; -.
DR HGNC; HGNC:18650; SPATS2.
DR HPA; ENSG00000123352; Low tissue specificity.
DR MIM; 611667; gene.
DR neXtProt; NX_Q86XZ4; -.
DR OpenTargets; ENSG00000123352; -.
DR PharmGKB; PA134871579; -.
DR VEuPathDB; HostDB:ENSG00000123352; -.
DR eggNOG; ENOG502QY9Y; Eukaryota.
DR GeneTree; ENSGT00390000001138; -.
DR HOGENOM; CLU_037089_0_0_1; -.
DR InParanoid; Q86XZ4; -.
DR OMA; SEWGQAH; -.
DR OrthoDB; 1523681at2759; -.
DR PhylomeDB; Q86XZ4; -.
DR TreeFam; TF320553; -.
DR PathwayCommons; Q86XZ4; -.
DR SignaLink; Q86XZ4; -.
DR BioGRID-ORCS; 65244; 16 hits in 1078 CRISPR screens.
DR ChiTaRS; SPATS2; human.
DR GenomeRNAi; 65244; -.
DR Pharos; Q86XZ4; Tdark.
DR PRO; PR:Q86XZ4; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q86XZ4; protein.
DR Bgee; ENSG00000123352; Expressed in ganglionic eminence and 187 other tissues.
DR ExpressionAtlas; Q86XZ4; baseline and differential.
DR Genevisible; Q86XZ4; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR InterPro; IPR009816; DUF1387.
DR InterPro; IPR009060; UBA-like_sf.
DR PANTHER; PTHR15623; PTHR15623; 1.
DR Pfam; PF07139; DUF1387; 1.
DR SUPFAM; SSF46934; SSF46934; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..545
FT /note="Spermatogenesis-associated serine-rich protein 2"
FT /id="PRO_0000307697"
FT REGION 85..137
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 197..216
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 369..545
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 105..126
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 369..438
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 454..520
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 143
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8K1N4"
FT MOD_RES 146
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19690332"
FT MOD_RES 148
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8K1N4"
FT MOD_RES 520
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT CONFLICT 393
FT /note="S -> N (in Ref. 2; BAB14958)"
FT /evidence="ECO:0000305"
FT CONFLICT 514
FT /note="G -> R (in Ref. 2; BAB14460)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 545 AA; 59545 MW; E203EA5BA3FDB0BF CRC64;
MSRKQNQKDS SGFIFDLQSN TVLAQGGAFE NMKEKINAVR AIVPNKSNNE IILVLQHFDN
CVDKTVQAFM EGSASEVLKE WTVTGKKKNK KKKNKPKPAA EPSNGIPDSS KSVSIQEEQS
APSSEKGGMN GYHVNGAIND TESVDSLSEG LETLSIDARE LEDPESAMLD TLDRTGSMLQ
NGVSDFETKS LTMHSIHNSQ QPRNAAKSLS RPTTETQFSN MGMEDVPLAT SKKLSSNIEK
SVKDLQRCTV SLARYRVVVK EEMDASIKKM KQAFAELESC LMDREVALLA EMDKVKAEAM
EILLSRQKKA ELLKKMTHVA VQMSEQQLVE LRADIKHFVS ERKYDEDLGR VARFTCDVET
LKKSIDSFGQ VSHPKNSYST RSRCSSVTSV SLSSPSDASA ASSSTCASPP SLTSANKKNF
APGETPAAIA NSSGQPYQPL REVLPGNRRG GQGYRPQGQK SNDPMNQGRH DSMGRYRNSS
WYSSGSRYQS APSQAPGNTI ERGQTHSAGT NGTGVSMEPS PPTPSFKKGL PQRKPRTSQT
EAVNS
