SPAS_SALTY
ID SPAS_SALTY Reviewed; 356 AA.
AC P40702;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Surface presentation of antigens protein SpaS;
GN Name=spaS; OrderedLocusNames=STM2887;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8404849; DOI=10.1002/j.1460-2075.1993.tb06056.x;
RA Groisman E.A., Ochman H.;
RT "Cognate gene clusters govern invasion of host epithelial cells by
RT Salmonella typhimurium and Shigella flexneri.";
RL EMBO J. 12:3779-3787(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
CC -!- FUNCTION: Involved in a secretory pathway responsible for the surface
CC presentation of determinants needed for the entry of Salmonella species
CC into mammalian cells.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the type III secretion exporter family.
CC {ECO:0000305}.
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DR EMBL; X73525; CAA51927.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL21767.1; -; Genomic_DNA.
DR PIR; S37356; S37356.
DR RefSeq; NP_461808.1; NC_003197.2.
DR RefSeq; WP_000097719.1; NC_003197.2.
DR PDB; 3C01; X-ray; 2.60 A; A/B/C/D=211-258, E/F/G/H=259-356.
DR PDBsum; 3C01; -.
DR AlphaFoldDB; P40702; -.
DR SMR; P40702; -.
DR DIP; DIP-59558N; -.
DR IntAct; P40702; 9.
DR STRING; 99287.STM2887; -.
DR MEROPS; N06.002; -.
DR TCDB; 3.A.6.1.3; the type iii (virulence-related) secretory pathway (iiisp) family.
DR PaxDb; P40702; -.
DR EnsemblBacteria; AAL21767; AAL21767; STM2887.
DR GeneID; 1254410; -.
DR KEGG; stm:STM2887; -.
DR PATRIC; fig|99287.12.peg.3043; -.
DR HOGENOM; CLU_041013_1_3_6; -.
DR OMA; QPNPGAY; -.
DR PhylomeDB; P40702; -.
DR BioCyc; SENT99287:STM2887-MON; -.
DR EvolutionaryTrace; P40702; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR Gene3D; 3.40.1690.10; -; 1.
DR InterPro; IPR006307; BsaZ.
DR InterPro; IPR006135; T3SS_substrate_exporter.
DR InterPro; IPR029025; T3SS_substrate_exporter_C.
DR PANTHER; PTHR30531; PTHR30531; 1.
DR Pfam; PF01312; Bac_export_2; 1.
DR PRINTS; PR00950; TYPE3IMSPROT.
DR SUPFAM; SSF160544; SSF160544; 1.
DR TIGRFAMs; TIGR01404; FlhB_rel_III; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Virulence.
FT CHAIN 1..356
FT /note="Surface presentation of antigens protein SpaS"
FT /id="PRO_0000180956"
FT TRANSMEM 29..49
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 72..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 261..281
FT /note="Helical"
FT /evidence="ECO:0000255"
FT HELIX 243..250
FT /evidence="ECO:0007829|PDB:3C01"
FT STRAND 253..257
FT /evidence="ECO:0007829|PDB:3C01"
FT STRAND 262..267
FT /evidence="ECO:0007829|PDB:3C01"
FT TURN 270..272
FT /evidence="ECO:0007829|PDB:3C01"
FT STRAND 277..283
FT /evidence="ECO:0007829|PDB:3C01"
FT HELIX 284..297
FT /evidence="ECO:0007829|PDB:3C01"
FT STRAND 301..303
FT /evidence="ECO:0007829|PDB:3C01"
FT HELIX 305..314
FT /evidence="ECO:0007829|PDB:3C01"
FT HELIX 323..342
FT /evidence="ECO:0007829|PDB:3C01"
SQ SEQUENCE 356 AA; 40093 MW; 5860259314317CF8 CRC64;
MSSNKTEKPT KKRLEDSAKK GQSFKSKDLI IACLTLGGIA YLVSYGSFNE FMGIIKIIIA
DNFDQSMADY SLAVFGIGLK YLIPFMLLCL VCSALPALLQ AGFVLATEAL KPNLSALNPV
EGAKKLFSMR TVKDTVKTLL YLSSFVVAAI ICWKKYKVEI FSQLNGNIVG IAVIWRELLL
ALVLTCLACA LIVLLLDAIA EYFLTMKDMK MDKEEVKREM KEQEGNPEVK SKRREVHMEI
LSEQVKSDIE NSRLIVANPT HITIGIYFKP ELMPIPMISV YETNQRALAV RAYAEKVGVP
VIVDIKLARS LFKTHRRYDL VSLEEIDEVL RLLVWLEEVE NAGKDVIQPQ ENEVRH
