SPAS_SHIFL
ID SPAS_SHIFL Reviewed; 342 AA.
AC P0A1M8; P40707;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 108.
DE RecName: Full=Surface presentation of antigens protein SpaS;
DE AltName: Full=Spa40 protein;
GN Name=spaS; Synonyms=spa40; OrderedLocusNames=CP0156;
OS Shigella flexneri.
OG Plasmid pWR100, Plasmid pMYSH6000, and Plasmid pCP301.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=YSH6000 / Serotype 2a; PLASMID=pMYSH6000;
RX PubMed=8385666; DOI=10.1128/jb.175.8.2334-2346.1993;
RA Sasakawa C., Komatsu K., Tobe T., Suzuki T., Yoshikawa M.;
RT "Eight genes in region 5 that form an operon are essential for invasion of
RT epithelial cells by Shigella flexneri 2a.";
RL J. Bacteriol. 175:2334-2346(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11115111; DOI=10.1046/j.1365-2958.2000.02179.x;
RA Buchrieser C., Glaser P., Rusniok C., Nedjari H., d'Hauteville H.,
RA Kunst F., Sansonetti P.J., Parsot C.;
RT "The virulence plasmid pWR100 and the repertoire of proteins secreted by
RT the type III secretion apparatus of Shigella flexneri.";
RL Mol. Microbiol. 38:760-771(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11292750; DOI=10.1128/iai.69.5.3271-3285.2001;
RA Venkatesan M.M., Goldberg M.B., Rose D.J., Grotbeck E.J., Burland V.,
RA Blattner F.R.;
RT "Complete DNA sequence and analysis of the large virulence plasmid of
RT Shigella flexneri.";
RL Infect. Immun. 69:3271-3285(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a; PLASMID=pCP301;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
CC -!- FUNCTION: Required for surface presentation of invasion plasmid
CC antigens. Could play a role in preserving the translocation competence
CC of the ipa antigens. Required for invasion and for secretion of the
CC three ipa proteins.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the type III secretion exporter family.
CC {ECO:0000305}.
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DR EMBL; D13663; BAA02832.1; -; Genomic_DNA.
DR EMBL; AL391753; CAC05831.1; -; Genomic_DNA.
DR EMBL; AF348706; AAK18475.1; -; Genomic_DNA.
DR EMBL; AF386526; AAL72303.1; -; Genomic_DNA.
DR PIR; A38908; A38908.
DR RefSeq; NP_085319.1; NC_002698.1.
DR RefSeq; NP_858289.1; NC_004851.1.
DR RefSeq; WP_001284686.1; NZ_WPGS01000043.1.
DR RefSeq; YP_009062513.1; NC_024996.1.
DR PDB; 2VT1; X-ray; 2.00 A; A=207-257, B=258-342.
DR PDBsum; 2VT1; -.
DR AlphaFoldDB; P0A1M8; -.
DR SMR; P0A1M8; -.
DR STRING; 198214.CP0156; -.
DR MEROPS; N06.002; -.
DR TCDB; 3.A.6.1.2; the type iii (virulence-related) secretory pathway (iiisp) family.
DR PRIDE; P0A1M8; -.
DR EnsemblBacteria; AAL72303; AAL72303; SF_p0156.
DR GeneID; 1237999; -.
DR KEGG; sfl:CP0156; -.
DR PATRIC; fig|198214.7.peg.5401; -.
DR HOGENOM; CLU_041013_1_3_6; -.
DR OMA; QPNPGAY; -.
DR EvolutionaryTrace; P0A1M8; -.
DR Proteomes; UP000001006; Plasmid pCP301.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR Gene3D; 3.40.1690.10; -; 1.
DR InterPro; IPR006307; BsaZ.
DR InterPro; IPR006135; T3SS_substrate_exporter.
DR InterPro; IPR029025; T3SS_substrate_exporter_C.
DR PANTHER; PTHR30531; PTHR30531; 1.
DR Pfam; PF01312; Bac_export_2; 1.
DR PRINTS; PR00950; TYPE3IMSPROT.
DR SUPFAM; SSF160544; SSF160544; 1.
DR TIGRFAMs; TIGR01404; FlhB_rel_III; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; Membrane; Plasmid;
KW Reference proteome; Transmembrane; Transmembrane helix; Virulence.
FT CHAIN 1..342
FT /note="Surface presentation of antigens protein SpaS"
FT /id="PRO_0000180957"
FT TRANSMEM 28..48
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..280
FT /note="Helical"
FT /evidence="ECO:0000255"
FT HELIX 242..249
FT /evidence="ECO:0007829|PDB:2VT1"
FT STRAND 252..256
FT /evidence="ECO:0007829|PDB:2VT1"
FT STRAND 260..266
FT /evidence="ECO:0007829|PDB:2VT1"
FT TURN 269..271
FT /evidence="ECO:0007829|PDB:2VT1"
FT STRAND 276..282
FT /evidence="ECO:0007829|PDB:2VT1"
FT HELIX 283..295
FT /evidence="ECO:0007829|PDB:2VT1"
FT STRAND 300..302
FT /evidence="ECO:0007829|PDB:2VT1"
FT HELIX 304..313
FT /evidence="ECO:0007829|PDB:2VT1"
FT STRAND 316..319
FT /evidence="ECO:0007829|PDB:2VT1"
FT TURN 322..324
FT /evidence="ECO:0007829|PDB:2VT1"
FT HELIX 325..337
FT /evidence="ECO:0007829|PDB:2VT1"
SQ SEQUENCE 342 AA; 39852 MW; 48BBF531BFE034BC CRC64;
MANKTEKPTP KKLKDAAKKG QSFKFKDLTT VVIILVGTFT IISFFSLSDV MLLYRYVIIN
DFEINEGKYF FAVVIVFFKI IGFPLFFCVL SAVLPTLVQT KFVLATKAIK IDFSVLNPVK
GLKKIFSIKT IKEFFKSILL LIILALTTYF FWINDRKIIF SQVFSSVDGL YLIWGRLFKD
IILFFLAFSI LVIILDFVIE FILYMKDMMM DKQEIKREYI EQEGHFETKS RRRELHIEIL
SEQTKSDIRN SKLVVMNPTH IAIGIYFNPE IAPAPFISLI ETNQCALAVR KYANEVGIPT
VRDVKLARKL YKTHTKYSFV DFEHLDEVLR LIVWLEQVEN TH
