ID   SPAS_SHISO              Reviewed;         342 AA.
AC   P0A1M9; P40707;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 52.
DE   RecName: Full=Surface presentation of antigens protein SpaS;
DE   AltName: Full=Spa40 protein;
GN   Name=spaS; Synonyms=spa40;
OS   Shigella sonnei.
OG   Plasmid pINV.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=624;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=HW383;
RA   Arakawa E., Kato J., Ito K., Watanabe H.;
RT   "Comparison and high conservation of nucleotide sequences of spa-mxi
RT   regions between S.sonnei and S.flexneri -- identification of a new gene
RT   coding plausible membrane protein.";
RL   Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for surface presentation of invasion plasmid
CC       antigens. Could play a role in preserving the translocation competence
CC       of the ipa antigens. Required for invasion and for secretion of the
CC       three ipa proteins (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the type III secretion exporter family.
CC       {ECO:0000305}.
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DR   EMBL; D50601; BAA09165.1; -; Genomic_DNA.
DR   AlphaFoldDB; P0A1M9; -.
DR   SMR; P0A1M9; -.
DR   STRING; 216599.GCA_000283715_05247; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR   Gene3D; 3.40.1690.10; -; 1.
DR   InterPro; IPR006307; BsaZ.
DR   InterPro; IPR006135; T3SS_substrate_exporter.
DR   InterPro; IPR029025; T3SS_substrate_exporter_C.
DR   PANTHER; PTHR30531; PTHR30531; 1.
DR   Pfam; PF01312; Bac_export_2; 1.
DR   PRINTS; PR00950; TYPE3IMSPROT.
DR   SUPFAM; SSF160544; SSF160544; 1.
DR   TIGRFAMs; TIGR01404; FlhB_rel_III; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Plasmid; Transmembrane;
KW   Transmembrane helix; Virulence.
FT   CHAIN           1..342
FT                   /note="Surface presentation of antigens protein SpaS"
FT                   /id="PRO_0000180958"
FT   TRANSMEM        28..48
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        70..90
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        133..153
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        158..178
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        181..201
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        260..280
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   342 AA;  39852 MW;  48BBF531BFE034BC CRC64;
     MANKTEKPTP KKLKDAAKKG QSFKFKDLTT VVIILVGTFT IISFFSLSDV MLLYRYVIIN
     DFEINEGKYF FAVVIVFFKI IGFPLFFCVL SAVLPTLVQT KFVLATKAIK IDFSVLNPVK
     GLKKIFSIKT IKEFFKSILL LIILALTTYF FWINDRKIIF SQVFSSVDGL YLIWGRLFKD
     IILFFLAFSI LVIILDFVIE FILYMKDMMM DKQEIKREYI EQEGHFETKS RRRELHIEIL
     SEQTKSDIRN SKLVVMNPTH IAIGIYFNPE IAPAPFISLI ETNQCALAVR KYANEVGIPT
     VRDVKLARKL YKTHTKYSFV DFEHLDEVLR LIVWLEQVEN TH