SPAT1_MOUSE
ID SPAT1_MOUSE Reviewed; 444 AA.
AC Q9D5R4; B9EI96; Q7TPN4;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Spermatogenesis-associated protein 1;
GN Name=Spata1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain, and Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP SUBCELLULAR LOCATION, INTERACTION WITH IFT20, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=31816150; DOI=10.1002/dvdy.141;
RA Zhang L., Zhen J., Huang Q., Liu H., Li W., Zhang S., Min J., Li Y.,
RA Shi L., Woods J., Chen X., Shi Y., Liu Y., Hess R.A., Song S., Zhang Z.;
RT "Mouse spermatogenesis-associated protein 1 (SPATA1), an IFT20 binding
RT partner, is an acrosomal protein.";
RL Dev. Dyn. 249:543-555(2020).
CC -!- SUBUNIT: Interacts with IFT20. {ECO:0000269|PubMed:31816150}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome
CC {ECO:0000269|PubMed:31816150}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9D5R4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9D5R4-2; Sequence=VSP_035225, VSP_035226;
CC -!- TISSUE SPECIFICITY: Highly abundant in the testis, and is also
CC expressed in the heart and kidney (at protein level).
CC {ECO:0000269|PubMed:31816150}.
CC -!- DEVELOPMENTAL STAGE: Detectable at postnatal day 24 (P24) and its
CC expression is increased at day P30 and P35.
CC {ECO:0000269|PubMed:31816150}.
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DR EMBL; AK015003; BAB29666.1; -; mRNA.
DR EMBL; CH466532; EDL11973.1; -; Genomic_DNA.
DR EMBL; BC055056; AAH55056.1; -; mRNA.
DR EMBL; BC139279; AAI39280.1; -; mRNA.
DR EMBL; BC139280; AAI39281.1; -; mRNA.
DR CCDS; CCDS38666.1; -. [Q9D5R4-1]
DR RefSeq; NP_001303685.1; NM_001316756.1. [Q9D5R4-1]
DR RefSeq; NP_081893.1; NM_027617.3. [Q9D5R4-1]
DR RefSeq; XP_006502153.1; XM_006502090.3. [Q9D5R4-1]
DR AlphaFoldDB; Q9D5R4; -.
DR SMR; Q9D5R4; -.
DR STRING; 10090.ENSMUSP00000029839; -.
DR PhosphoSitePlus; Q9D5R4; -.
DR jPOST; Q9D5R4; -.
DR MaxQB; Q9D5R4; -.
DR PaxDb; Q9D5R4; -.
DR PRIDE; Q9D5R4; -.
DR ProteomicsDB; 257305; -. [Q9D5R4-1]
DR ProteomicsDB; 257306; -. [Q9D5R4-2]
DR Antibodypedia; 78709; 17 antibodies from 7 providers.
DR DNASU; 70951; -.
DR Ensembl; ENSMUST00000029839; ENSMUSP00000029839; ENSMUSG00000028188. [Q9D5R4-1]
DR Ensembl; ENSMUST00000093951; ENSMUSP00000091483; ENSMUSG00000028188. [Q9D5R4-2]
DR Ensembl; ENSMUST00000197980; ENSMUSP00000142800; ENSMUSG00000028188. [Q9D5R4-1]
DR GeneID; 70951; -.
DR KEGG; mmu:70951; -.
DR UCSC; uc008rri.1; mouse. [Q9D5R4-1]
DR UCSC; uc008rrj.1; mouse. [Q9D5R4-2]
DR CTD; 100505741; -.
DR MGI; MGI:1918201; Spata1.
DR VEuPathDB; HostDB:ENSMUSG00000028188; -.
DR eggNOG; ENOG502QT5V; Eukaryota.
DR GeneTree; ENSGT00390000003298; -.
DR HOGENOM; CLU_2120304_0_0_1; -.
DR InParanoid; Q9D5R4; -.
DR OMA; RVYPDIT; -.
DR OrthoDB; 657461at2759; -.
DR PhylomeDB; Q9D5R4; -.
DR TreeFam; TF328372; -.
DR BioGRID-ORCS; 70951; 4 hits in 72 CRISPR screens.
DR ChiTaRS; Spata1; mouse.
DR PRO; PR:Q9D5R4; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q9D5R4; protein.
DR Bgee; ENSMUSG00000028188; Expressed in seminiferous tubule of testis and 125 other tissues.
DR ExpressionAtlas; Q9D5R4; baseline and differential.
DR Genevisible; Q9D5R4; MM.
DR GO; GO:0001669; C:acrosomal vesicle; IDA:UniProtKB.
DR GO; GO:0031410; C:cytoplasmic vesicle; IDA:MGI.
DR InterPro; IPR039062; SPAT1.
DR InterPro; IPR031478; SPATA1_C.
DR PANTHER; PTHR14421; PTHR14421; 1.
DR Pfam; PF15743; SPATA1_C; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasmic vesicle; Reference proteome.
FT CHAIN 1..444
FT /note="Spermatogenesis-associated protein 1"
FT /id="PRO_0000349216"
FT COILED 268..403
FT /evidence="ECO:0000255"
FT VAR_SEQ 106..114
FT /note="ALQPELYLL -> VCTMMLNSH (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_035225"
FT VAR_SEQ 115..444
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_035226"
SQ SEQUENCE 444 AA; 51077 MW; 07698646F286EAC3 CRC64;
MSLNPSRPSS SELVELHVFY VPEGSWNYKL NTISIEVINN FISAGFIRVS PQLTLQALRE
RLGEFLGVDA VAEKFLFLKC IGNNLAVVKE KQESELKLKS FAPPYALQPE LYLLPVIDHL
GNVYSASTVT LDEQESINDT TEINGTIYRP DSVSLSKDEP GNPSLLENTW RDFTNQEEAE
ESQPTQNHFG NSKLLGSLEE SNDYFGNLKS PFLWKNDDEE EDEEKDNAAL SRRQATLVCD
KECTALPDLI DFPSFPSQRV SSRLTDTSLL KIEREKIIEQ MKQVKEERKY LENIREELIK
KVDKLFEQNK SKRYHASDSW KKKYLDTKKV TASLEEVLTK LREDLELYYK KLLMQLEARE
IKMRPRNLAN ISDSKNYLII QITEVQHAID QLKRKLDTDK MKLILEVKMR KQAVSDLQTL
KADLTQKKMG APFRPPMFSG SVPT
