ID   SPAT2_DANRE             Reviewed;         550 AA.
AC   F1QCY8; A1YAC7; A7E269;
DT   10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT   03-MAY-2011, sequence version 1.
DT   03-AUG-2022, entry version 53.
DE   RecName: Full=Spermatogenesis-associated protein 2 {ECO:0000305};
GN   Name=spata2 {ECO:0000312|ZFIN:ZDB-GENE-070822-5};
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RX   PubMed=17486545; DOI=10.1387/ijdb.062220em;
RA   Moro E., Maran C., Slongo M.L., Argenton F., Toppo S., Onisto M.;
RT   "Zebrafish spata2 is expressed at early developmental stages.";
RL   Int. J. Dev. Biol. 51:241-246(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Intestine;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Bridging factor that mediates the recruitment of cyld to the
CC       LUBAC complex, thereby regulating TNF-alpha-induced necroptosis.
CC       Required to activate the 'Met-1'- (linear) and 'Lys-63'-linked
CC       deubiquitinase activities of cyld. {ECO:0000250|UniProtKB:Q9UM82}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9UM82}. Nucleus
CC       {ECO:0000250|UniProtKB:Q66HP6}.
CC   -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC       Detected at two-cell stage. Ubiquitously expressed from the shield
CC       stage to 24 hours post-fertilization (hpf), and then becomes
CC       preferentially expressed in the central nervous system. From 5 days
CC       post-fertilization (dpf), expression becomes detectable in the gut and
CC       pronephric duct epithelium. {ECO:0000269|PubMed:17486545}.
CC   -!- SIMILARITY: Belongs to the SPATA2 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; DQ869310; ABI74625.1; -; mRNA.
DR   EMBL; CR759920; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC150216; AAI50217.1; -; mRNA.
DR   RefSeq; NP_001073660.2; NM_001080191.2.
DR   RefSeq; XP_005161965.1; XM_005161908.3.
DR   RefSeq; XP_005161966.1; XM_005161909.3.
DR   AlphaFoldDB; F1QCY8; -.
DR   SMR; F1QCY8; -.
DR   STRING; 7955.ENSDARP00000086690; -.
DR   PaxDb; F1QCY8; -.
DR   Ensembl; ENSDART00000092258; ENSDARP00000086690; ENSDARG00000063250.
DR   GeneID; 568830; -.
DR   KEGG; dre:568830; -.
DR   CTD; 9825; -.
DR   ZFIN; ZDB-GENE-070822-5; spata2.
DR   eggNOG; ENOG502QQYQ; Eukaryota.
DR   GeneTree; ENSGT00530000063956; -.
DR   HOGENOM; CLU_039585_0_0_1; -.
DR   InParanoid; F1QCY8; -.
DR   OMA; RTHLAKE; -.
DR   OrthoDB; 461395at2759; -.
DR   TreeFam; TF328840; -.
DR   PRO; PR:F1QCY8; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 23.
DR   Bgee; ENSDARG00000063250; Expressed in retina and 31 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0012501; P:programmed cell death; IEA:UniProtKB-KW.
DR   GO; GO:0070536; P:protein K63-linked deubiquitination; ISS:UniProtKB.
DR   GO; GO:1990108; P:protein linear deubiquitination; ISS:UniProtKB.
DR   GO; GO:0050727; P:regulation of inflammatory response; ISS:UniProtKB.
DR   GO; GO:0060544; P:regulation of necroptotic process; ISS:UniProtKB.
DR   GO; GO:0010803; P:regulation of tumor necrosis factor-mediated signaling pathway; ISS:UniProtKB.
DR   InterPro; IPR036339; PUB-like_dom_sf.
DR   InterPro; IPR029744; SPATA2.
DR   PANTHER; PTHR15326:SF8; PTHR15326:SF8; 1.
DR   SUPFAM; SSF143503; SSF143503; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Necrosis; Nucleus; Reference proteome.
FT   CHAIN           1..550
FT                   /note="Spermatogenesis-associated protein 2"
FT                   /id="PRO_0000445576"
FT   DOMAIN          83..156
FT                   /note="PUB"
FT                   /evidence="ECO:0000255"
FT   REGION          457..480
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           320..337
FT                   /note="PIM motif"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UM82"
FT   COMPBIAS        466..480
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        246..265
FT                   /note="DVTDSAGHWHQASKPVLKAS -> RPLDIDRAHLRRSGRPSKSA (in
FT                   Ref. 1; ABI74625)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        297
FT                   /note="A -> V (in Ref. 3; AAI50217)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   550 AA;  60648 MW;  890EA182A6CD1F5E CRC64;
     MDAKLREDLF RRYVASLENR LEEGAAEGGG DRQGAKTEEA LISTATALLG SYQADPGQRF
     RMLRFYDVVE NSLRTQRGTS LRTVGTAFAT LETICTNLLL FPWKKEYRCI KTFTGPYVYQ
     LQSVLCDSDL RSLLRSMGYS REQELQYNVR DHPGGASHLR QLAFELLLAQ AECRLLGEVV
     SMSRGFASEL EAVELRRNTR EDAAGCADAL RRRDSLTGDL SRLSVRPLDI DRAHLRRSGR
     PSKSVDVTDS AGHWHQASKP VLKASLSLRK EPLFVDTEED MKDEIIRPSP SLYPVAAPPS
     YSPVADFFPI QSPPSEPYSY HLSSLDEVDL YTERGLGGHQ TPSRPPSREP RDSWVLKGHM
     MKCQGCGMGC PSLSSCQRCD MILCSSCHAV DPAPCCGFQD YTKTSRPLDG YMPIKEKLSV
     YSSAHSHTHP HPHPLPHAQS HSLLLDKAVM SSKLFPSKPV GSGPSPVGSL VSSGSSSSGG
     ERLSVGGSRC GFCNKPGASH TCVNCSKVSC DTCMSLYASD LCTRKNPHHN FVPNHQLNFK
     SSTISHLVYR