ID   SPAT4_MOUSE             Reviewed;         295 AA.
AC   Q8K3V1; Q923U8;
DT   10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Spermatogenesis-associated protein 4;
DE   AltName: Full=Spermatogenesis-related gene 2 protein;
DE   AltName: Full=Testis and spermatogenesis cell-related protein 2;
DE   AltName: Full=Testis spermatocyte apoptosis-related gene 2 protein;
GN   Name=Spata4; Synonyms=Srg2, Tsarg2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   TISSUE=Testis;
RX   PubMed=12417927;
RA   Liu S.F., Li L.Y., Fu J.J., Liu G., Xing X.W., Lu G.X.;
RT   "Molecular cloning of SRG2, a mouse testis spermatocyte apoptosis-related
RT   gene.";
RL   Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 34:796-799(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: May play a role in apoptosis regulation.
CC       {ECO:0000250|UniProtKB:Q8NEY3}.
CC   -!- INTERACTION:
CC       Q8K3V1; Q9CQP2: Trappc2; NbExp=3; IntAct=EBI-7067375, EBI-1172267;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8NEY3}.
CC   -!- TISSUE SPECIFICITY: Testis. {ECO:0000269|PubMed:12417927}.
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DR   EMBL; AF530515; AAM94341.1; -; mRNA.
DR   EMBL; AF395083; AAK77865.2; -; mRNA.
DR   EMBL; BC048650; AAH48650.1; -; mRNA.
DR   CCDS; CCDS22308.1; -.
DR   RefSeq; NP_598472.2; NM_133711.3.
DR   AlphaFoldDB; Q8K3V1; -.
DR   SMR; Q8K3V1; -.
DR   IntAct; Q8K3V1; 4.
DR   MINT; Q8K3V1; -.
DR   STRING; 10090.ENSMUSP00000033917; -.
DR   PhosphoSitePlus; Q8K3V1; -.
DR   PaxDb; Q8K3V1; -.
DR   PRIDE; Q8K3V1; -.
DR   ProteomicsDB; 257335; -.
DR   Antibodypedia; 17256; 149 antibodies from 26 providers.
DR   DNASU; 69281; -.
DR   Ensembl; ENSMUST00000033917; ENSMUSP00000033917; ENSMUSG00000031518.
DR   GeneID; 69281; -.
DR   KEGG; mmu:69281; -.
DR   UCSC; uc009lsf.1; mouse.
DR   CTD; 132851; -.
DR   MGI; MGI:1916531; Spata4.
DR   VEuPathDB; HostDB:ENSMUSG00000031518; -.
DR   eggNOG; ENOG502QU8V; Eukaryota.
DR   GeneTree; ENSGT00910000144159; -.
DR   HOGENOM; CLU_077979_1_0_1; -.
DR   InParanoid; Q8K3V1; -.
DR   OMA; SYQMHLP; -.
DR   OrthoDB; 1471531at2759; -.
DR   PhylomeDB; Q8K3V1; -.
DR   TreeFam; TF323506; -.
DR   BioGRID-ORCS; 69281; 2 hits in 72 CRISPR screens.
DR   ChiTaRS; Spata4; mouse.
DR   PRO; PR:Q8K3V1; -.
DR   Proteomes; UP000000589; Chromosome 8.
DR   RNAct; Q8K3V1; protein.
DR   Bgee; ENSMUSG00000031518; Expressed in seminiferous tubule of testis and 18 other tissues.
DR   Genevisible; Q8K3V1; MM.
DR   GO; GO:0005930; C:axoneme; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR   GO; GO:0051493; P:regulation of cytoskeleton organization; IBA:GO_Central.
DR   Gene3D; 1.10.418.10; -; 1.
DR   InterPro; IPR001715; CH-domain.
DR   InterPro; IPR010441; CH_2.
DR   InterPro; IPR036872; CH_dom_sf.
DR   Pfam; PF06294; CH_2; 1.
DR   PROSITE; PS50021; CH; 1.
PE   1: Evidence at protein level;
KW   Nucleus; Reference proteome.
FT   CHAIN           1..295
FT                   /note="Spermatogenesis-associated protein 4"
FT                   /id="PRO_0000072102"
FT   DOMAIN          48..154
FT                   /note="Calponin-homology (CH)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT   REGION          1..34
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          251..295
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        259..284
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   295 AA;  33579 MW;  6C4C1ECECC2A597E CRC64;
     MAAAGQAEEC LPLPAAESSK TSLPTPPAVP AGKKPKKCLV YPHPPRSSRL SRSVLRWLQG
     LDLSFFPRNV TRDFSNGYLV AEIFCIYYPW DLRLSSFENG TSLKVKLDNW AQIEKFLAKK
     KFKLPKELIH GTIHCKAGVP EILIQEIYTL LTHQEIRSIQ DDLANFTDYI YQMRLPLVPR
     NTVSKSIKNN IRLSELLSNP NVLSNELKIE FLILLQMLQR KLSRKLNPGW FDVKPTVGEI
     TIDRLPAHSY KRRYKSRGSK EKAAQPLSKS DNDGNARKEI HVKQSGNPCE NTENL