SPAT6_RAT
ID SPAT6_RAT Reviewed; 488 AA.
AC Q99MU5; A0JN12; Q9EQU0;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 2.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=Spermatogenesis-associated protein 6;
DE AltName: Full=Kinesin-related protein;
DE AltName: Full=Spermatogenesis-related factor 1;
DE Flags: Precursor;
GN Name=Spata6; Synonyms=Hash, Srf1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley;
RX PubMed=12771232; DOI=10.1093/molehr/gag047;
RA Oh C., Aho H., Shamsadin R., Nayernia K., Mueller C., Sancken U.,
RA Szpirer C., Engel W., Adham I.M.;
RT "Characterization, expression pattern and chromosomal localization of the
RT spermatogenesis associated 6 gene (Spata6).";
RL Mol. Hum. Reprod. 9:321-330(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-431.
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 259-488, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=11735130; DOI=10.1006/bbrc.2001.6048;
RA Yamano Y., Ohyama K., Sano T., Ohta M., Shimada A., Hirakawa Y.,
RA Sugimoto M., Morishima I.;
RT "A novel spermatogenesis-related factor-1 gene expressed in maturing rat
RT testis.";
RL Biochem. Biophys. Res. Commun. 289:888-893(2001).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-217; SER-219; SER-265;
RP SER-274; SER-325; SER-343; SER-346; SER-354; SER-424; SER-465 AND SER-487,
RP AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Required for formation of the sperm connecting piece during
CC spermiogenesis. Sperm connecting piece is essential for linking the
CC developing flagellum to the head during late spermiogenesis. May be
CC involved in myosin-based microfilament transport through interaction
CC with myosin subunits. {ECO:0000250|UniProtKB:Q3U6K5}.
CC -!- SUBUNIT: Interacts with MYL6. {ECO:0000250|UniProtKB:Q3U6K5}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q3U6K5}. Cell
CC projection, cilium, flagellum {ECO:0000250|UniProtKB:Q3U6K5}.
CC Note=Specifically localizes to the segmented columns and the capitulum
CC of the sperm connecting piece. {ECO:0000250|UniProtKB:Q3U6K5}.
CC -!- TISSUE SPECIFICITY: Testis-specific, in spermatocytes.
CC {ECO:0000269|PubMed:11735130}.
CC -!- DEVELOPMENTAL STAGE: Expression, first detected at 5 weeks of age,
CC increases up to 15 weeks and remains high for at least 63 weeks.
CC {ECO:0000269|PubMed:11735130}.
CC -!- SIMILARITY: Belongs to the SPATA6 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK20996.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAB20802.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF291466; AAK20996.1; ALT_INIT; mRNA.
DR EMBL; BC126081; AAI26082.1; -; mRNA.
DR EMBL; AB046606; BAB20802.1; ALT_INIT; mRNA.
DR AlphaFoldDB; Q99MU5; -.
DR STRING; 10116.ENSRNOP00000010524; -.
DR iPTMnet; Q99MU5; -.
DR PhosphoSitePlus; Q99MU5; -.
DR PaxDb; Q99MU5; -.
DR PRIDE; Q99MU5; -.
DR UCSC; RGD:628657; rat.
DR RGD; 628657; Spata6.
DR eggNOG; ENOG502QRV3; Eukaryota.
DR InParanoid; Q99MU5; -.
DR PhylomeDB; Q99MU5; -.
DR PRO; PR:Q99MU5; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0097224; C:sperm connecting piece; ISS:UniProtKB.
DR GO; GO:0032027; F:myosin light chain binding; ISO:RGD.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0044458; P:motile cilium assembly; ISS:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR InterPro; IPR027207; Spata6.
DR InterPro; IPR042769; SPATA6_fam.
DR InterPro; IPR032732; SPATA6_N.
DR PANTHER; PTHR16435; PTHR16435; 1.
DR PANTHER; PTHR16435:SF3; PTHR16435:SF3; 1.
DR Pfam; PF14909; SPATA6; 1.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Developmental protein; Differentiation; Flagellum;
KW Isopeptide bond; Phosphoprotein; Reference proteome; Secreted; Signal;
KW Spermatogenesis; Ubl conjugation.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..488
FT /note="Spermatogenesis-associated protein 6"
FT /id="PRO_0000278443"
FT REGION 175..225
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 198..220
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 217
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 219
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 265
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 274
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 325
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 343
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 346
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 354
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 424
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 465
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 487
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT CROSSLNK 248
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q9NWH7"
FT CONFLICT 24
FT /note="L -> V (in Ref. 2; AAI26082)"
FT /evidence="ECO:0000305"
FT CONFLICT 475
FT /note="Q -> K (in Ref. 3; BAB20802)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 488 AA; 56019 MW; CB34C041A185D570 CRC64;
MPKVKALQCA LALEIRSVTC PGVLLKDKED IYLSICVFGQ YKKTQCVPAT FPLVFNARMV
FEKVFPEAVD PGDVVAQLEY DTAVFELIQL VPPVGETLST YDENTRDFMF PGPNQISGHH
DSNRQVTMRR ISGLRGIAPK LEFSTTSVIT ECLISSRKCR TQDKFTYHSA PVEKPHGRLQ
CRTSRSQKKK SKSPERSKYC INTKNYEQPT ISSKSHSPSP YTKRRMCELS EDTRRRLAHL
NLGPYEFKKE TDKPPFVIRH VDPPSPRADN FFGSPGRDCE RDGWVRMHSD HPHLGCCRAK
DYKVIRSPHG RDFEDPFERC EDYLSPRTCS KPQHSARTLL VHSAPSTTPK HCASPVLNRA
SLRERFHSDW CSPPNCDEIH DRVKDVLKSH QAHGRHLCEE RDPEKEDELE LKRSLLYRDS
AYDSDPEYSS FQRPRGSFHL DDGECWSNRA ASCKGKSHRP VFENSMDKMY RNLYQKACSS
VSHTQESF
