SPB4_YARLI
ID SPB4_YARLI Reviewed; 626 AA.
AC Q6C193;
DT 04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=ATP-dependent rRNA helicase SPB4;
DE EC=3.6.4.-;
GN Name=SPB4; OrderedLocusNames=YALI0F18238g;
OS Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Dipodascaceae; Yarrowia.
OX NCBI_TaxID=284591;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CLIB 122 / E 150;
RX PubMed=15229592; DOI=10.1038/nature02579;
RA Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA Weissenbach J., Wincker P., Souciet J.-L.;
RT "Genome evolution in yeasts.";
RL Nature 430:35-44(2004).
CC -!- FUNCTION: ATP-binding RNA helicase involved in the biogenesis of 60S
CC ribosomal subunits. Required for the normal formation of 18S rRNA
CC through the processing of pre-rRNAs at sites A0, A1 and A2, and the
CC normal formation of 25S and 5.8S rRNAs through the processing of pre-
CC rRNAs at sites C1 and C2 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX55/SPB4
CC subfamily. {ECO:0000305}.
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DR EMBL; CR382132; CAG78378.1; -; Genomic_DNA.
DR RefSeq; XP_505569.1; XM_505569.1.
DR AlphaFoldDB; Q6C193; -.
DR SMR; Q6C193; -.
DR STRING; 4952.CAG78378; -.
DR EnsemblFungi; CAG78378; CAG78378; YALI0_F18238g.
DR GeneID; 2908671; -.
DR KEGG; yli:YALI0F18238g; -.
DR VEuPathDB; FungiDB:YALI0_F18238g; -.
DR HOGENOM; CLU_003041_26_4_1; -.
DR InParanoid; Q6C193; -.
DR OMA; DRAIHDK; -.
DR Proteomes; UP000001300; Chromosome F.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:InterPro.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR025313; DUF4217.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF13959; DUF4217; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM01178; DUF4217; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 3: Inferred from homology;
KW ATP-binding; Coiled coil; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing.
FT CHAIN 1..626
FT /note="ATP-dependent rRNA helicase SPB4"
FT /id="PRO_0000232334"
FT DOMAIN 34..239
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 273..437
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 549..626
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 523..604
FT /evidence="ECO:0000255"
FT MOTIF 3..31
FT /note="Q motif"
FT MOTIF 187..190
FT /note="DEAD box"
FT COMPBIAS 561..626
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 47..54
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 626 AA; 71091 MW; 629C7DA23D26AEC4 CRC64;
MSTDWKDLPV APWLIDTMEQ FGFTDMTPVQ ASVIPMFAGN KDVIVEAVTG SGKTIAFLVP
LIQRMLNLLK EGPAVSGRVY SVVVSPTREL ARQTYEVLQS ILEMGCPEAD ASDKITLEKK
KKGKAAPTMP KKIRGQLIMG GDLPSHMDLK NFLRDKPQII VATPGRLLEL LRAPQIKTSA
FDSLVLDEAD RLLDLGFGRD ITSIINILPK QRRTGLFSAT ITDAIQNLVK IGLRNPVKIV
VKVGGKKEQK TPLSLGLSYV VLEPREKLAY ALNLLSIYPY RKAIVYLPTC AAVTYYQQMF
SHLNEGREEP YEIYGLHGKL PSSTRIKILN KYQKTGAQAI LMTTDIAARG LDIPEVDLVV
QLDPPSDADT FQHRCGRAGR AGRQGQAIVL LHKGREEEYV DLMRVRKVKL RPYTGPGSEL
EGEKLDQEAT ELYTKLRKWV LEDRQRHDQA LLSFVSFVRF YSKHVAQSIF RIQSLDLPGL
AASYGLLRLP KMPELRTKDN ERPENTWLGE VIDFDTYSYK NPAKEEARLK ELEAHKEAMK
NKVKIHKTKN DVQKANRAWS STLQKKEEKS ERHQKKQTRI NEKIKRDFEE AKDLKQEESK
EVVNDWKDMV KKSKKRKVDL PTFDDL
