SPB9_HUMAN
ID SPB9_HUMAN Reviewed; 376 AA.
AC P50453; B2RBW3; Q5TD03;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 194.
DE RecName: Full=Serpin B9;
DE AltName: Full=Cytoplasmic antiproteinase 3;
DE Short=CAP-3;
DE Short=CAP3;
DE AltName: Full=Peptidase inhibitor 9;
DE Short=PI-9;
GN Name=SERPINB9; Synonyms=PI9;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Placenta;
RX PubMed=8530382; DOI=10.1074/jbc.270.50.29854;
RA Sprecher C.A., Morgenstern K.A., Mathewes S., Dahlen J.R., Schrader S.K.,
RA Foster D.C., Kisiel W.;
RT "Molecular cloning, expression, and partial characterization of two novel
RT members of the ovalbumin family of serine proteinase inhibitors.";
RL J. Biol. Chem. 270:29854-29861(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Bone marrow, and Placenta;
RX PubMed=8910377; DOI=10.1074/jbc.271.44.27802;
RA Sun J., Bird C.H., Sutton V., McDonald L., Coughlin P.B., Jong T.A.,
RA Trapani J.A., Bird P.I.;
RT "A cytosolic granzyme B inhibitor related to the viral apoptotic regulator
RT cytokine response modifier A is present in cytotoxic lymphocytes.";
RL J. Biol. Chem. 271:27802-27809(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Tanaka K., Harashima N., Shichijo S., Harada M., Itoh K.;
RT "Serine proteinase inhibitor 9 as a tumor antigen recognized by cytotoxic T
RT lymphocytes of epithelial cancer patients.";
RL Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [11]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [12]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [13]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- FUNCTION: Granzyme B inhibitor.
CC -!- INTERACTION:
CC P50453; O00463: TRAF5; NbExp=6; IntAct=EBI-711626, EBI-523498;
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the serpin family. Ov-serpin subfamily.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L40378; AAC41940.1; -; mRNA.
DR EMBL; U71364; AAC50793.1; -; mRNA.
DR EMBL; AB060690; BAB91078.1; -; mRNA.
DR EMBL; AK314842; BAG37360.1; -; mRNA.
DR EMBL; BT006673; AAP35319.1; -; mRNA.
DR EMBL; CR407627; CAG28555.1; -; mRNA.
DR EMBL; AL133351; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471087; EAW55093.1; -; Genomic_DNA.
DR EMBL; BC002538; AAH02538.1; -; mRNA.
DR CCDS; CCDS4478.1; -.
DR PIR; B59273; B59273.
DR RefSeq; NP_004146.1; NM_004155.5.
DR RefSeq; XP_005249241.1; XM_005249184.4.
DR AlphaFoldDB; P50453; -.
DR SMR; P50453; -.
DR BioGRID; 111290; 52.
DR IntAct; P50453; 31.
DR STRING; 9606.ENSP00000370074; -.
DR DrugBank; DB00481; Raloxifene.
DR MEROPS; I04.014; -.
DR GlyGen; P50453; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; P50453; -.
DR PhosphoSitePlus; P50453; -.
DR SwissPalm; P50453; -.
DR BioMuta; SERPINB9; -.
DR DMDM; 1709896; -.
DR OGP; P50453; -.
DR REPRODUCTION-2DPAGE; IPI00032139; -.
DR EPD; P50453; -.
DR jPOST; P50453; -.
DR MassIVE; P50453; -.
DR MaxQB; P50453; -.
DR PaxDb; P50453; -.
DR PeptideAtlas; P50453; -.
DR PRIDE; P50453; -.
DR ProteomicsDB; 56228; -.
DR Antibodypedia; 9285; 414 antibodies from 38 providers.
DR DNASU; 5272; -.
DR Ensembl; ENST00000380698.5; ENSP00000370074.4; ENSG00000170542.6.
DR GeneID; 5272; -.
DR KEGG; hsa:5272; -.
DR MANE-Select; ENST00000380698.5; ENSP00000370074.4; NM_004155.6; NP_004146.1.
DR UCSC; uc003mug.5; human.
DR CTD; 5272; -.
DR DisGeNET; 5272; -.
DR GeneCards; SERPINB9; -.
DR HGNC; HGNC:8955; SERPINB9.
DR HPA; ENSG00000170542; Tissue enhanced (lymphoid tissue, placenta).
DR MIM; 601799; gene.
DR neXtProt; NX_P50453; -.
DR OpenTargets; ENSG00000170542; -.
DR PharmGKB; PA35519; -.
DR VEuPathDB; HostDB:ENSG00000170542; -.
DR eggNOG; KOG2392; Eukaryota.
DR GeneTree; ENSGT00940000154931; -.
DR HOGENOM; CLU_023330_0_2_1; -.
DR InParanoid; P50453; -.
DR OMA; FEKFTAW; -.
DR OrthoDB; 1124079at2759; -.
DR PhylomeDB; P50453; -.
DR TreeFam; TF352619; -.
DR PathwayCommons; P50453; -.
DR SignaLink; P50453; -.
DR BioGRID-ORCS; 5272; 7 hits in 1081 CRISPR screens.
DR ChiTaRS; SERPINB9; human.
DR GeneWiki; SERPINB9; -.
DR GenomeRNAi; 5272; -.
DR Pharos; P50453; Tbio.
DR PRO; PR:P50453; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; P50453; protein.
DR Bgee; ENSG00000170542; Expressed in epithelium of nasopharynx and 174 other tissues.
DR ExpressionAtlas; P50453; baseline and differential.
DR Genevisible; P50453; HS.
DR GO; GO:0062023; C:collagen-containing extracellular matrix; HDA:BHF-UCL.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; IDA:HPA.
DR GO; GO:0016020; C:membrane; HDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0043027; F:cysteine-type endopeptidase inhibitor activity involved in apoptotic process; IDA:UniProtKB.
DR GO; GO:0002020; F:protease binding; IPI:UniProtKB.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IDA:UniProtKB.
DR GO; GO:0071391; P:cellular response to estrogen stimulus; IEP:UniProtKB.
DR GO; GO:0006955; P:immune response; IDA:UniProtKB.
DR GO; GO:0002448; P:mast cell mediated immunity; IEP:UniProtKB.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB.
DR GO; GO:0010951; P:negative regulation of endopeptidase activity; IMP:UniProtKB.
DR GO; GO:0042270; P:protection from natural killer cell mediated cytotoxicity; IMP:UniProtKB.
DR Gene3D; 2.30.39.10; -; 2.
DR Gene3D; 3.30.497.10; -; 1.
DR InterPro; IPR000240; Serpin_B9/Maspin.
DR InterPro; IPR023795; Serpin_CS.
DR InterPro; IPR023796; Serpin_dom.
DR InterPro; IPR000215; Serpin_fam.
DR InterPro; IPR036186; Serpin_sf.
DR InterPro; IPR042178; Serpin_sf_1.
DR InterPro; IPR042185; Serpin_sf_2.
DR PANTHER; PTHR11461; PTHR11461; 1.
DR Pfam; PF00079; Serpin; 1.
DR PRINTS; PR00676; MASPIN.
DR SMART; SM00093; SERPIN; 1.
DR SUPFAM; SSF56574; SSF56574; 1.
DR PROSITE; PS00284; SERPIN; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Protease inhibitor; Reference proteome;
KW Serine protease inhibitor.
FT CHAIN 1..376
FT /note="Serpin B9"
FT /id="PRO_0000094113"
FT SITE 340..341
FT /note="Reactive bond"
FT /evidence="ECO:0000250"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22223895,
FT ECO:0007744|PubMed:22814378"
SQ SEQUENCE 376 AA; 42404 MW; ECBA80869B939753 CRC64;
METLSNASGT FAIRLLKILC QDNPSHNVFC SPVSISSALA MVLLGAKGNT ATQMAQALSL
NTEEDIHRAF QSLLTEVNKA GTQYLLRTAN RLFGEKTCQF LSTFKESCLQ FYHAELKELS
FIRAAEESRK HINTWVSKKT EGKIEELLPG SSIDAETRLV LVNAIYFKGK WNEPFDETYT
REMPFKINQE EQRPVQMMYQ EATFKLAHVG EVRAQLLELP YARKELSLLV LLPDDGVELS
TVEKSLTFEK LTAWTKPDCM KSTEVEVLLP KFKLQEDYDM ESVLRHLGIV DAFQQGKADL
SAMSAERDLC LSKFVHKSFV EVNEEGTEAA AASSCFVVAE CCMESGPRFC ADHPFLFFIR
HNRANSILFC GRFSSP
