SPC24_ARATH
ID SPC24_ARATH Reviewed; 201 AA.
AC Q67XT3; Q9SR88;
DT 23-MAY-2018, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Kinetochore protein SPC24 homolog {ECO:0000303|PubMed:29356153};
DE Short=AtSPC24 {ECO:0000303|PubMed:29356153};
DE AltName: Full=Protein MERISTEM UNSTRUCTURED {ECO:0000303|PubMed:29356153};
GN Name=SPC24 {ECO:0000303|PubMed:29356153};
GN Synonyms=MUN {ECO:0000303|PubMed:29356153};
GN OrderedLocusNames=At3g08880 {ECO:0000312|Araport:AT3G08880};
GN ORFNames=T16O11.19 {ECO:0000312|EMBL:AAF07836.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-107.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=29356153; DOI=10.1111/tpj.13823;
RA Shin J., Jeong G., Park J.Y., Kim H., Lee I.;
RT "MUN (MERISTEM UNSTRUCTURED), encoding a SPC24 homolog of NDC80 kinetochore
RT complex, affects development through cell division in Arabidopsis
RT thaliana.";
RL Plant J. 93:977-991(2018).
CC -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC NDC80 complex, which is required for chromosome segregation and spindle
CC checkpoint activity to ensure proper cell division. Required for the
CC maintenance of plant architecture. {ECO:0000269|PubMed:29356153}.
CC -!- SUBUNIT: Component of the NDC80 complex, which consists of NDC80, NUF2,
CC SPC24 and SPC25. {ECO:0000269|PubMed:29356153}.
CC -!- SUBCELLULAR LOCATION: Chromosome, centromere
CC {ECO:0000269|PubMed:29356153}. Note=Colocalizes with CENH3 at the
CC centromere. {ECO:0000269|PubMed:29356153}.
CC -!- TISSUE SPECIFICITY: Highly expressed in actively dividing tissues, such
CC as shoot apical meristem (SAM), root apical meristem (RAM),
CC vasculature, newly emerging leaves and inflorescence shoots.
CC {ECO:0000269|PubMed:29356153}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethality due to division arrest before
CC the globular stage. {ECO:0000269|PubMed:29356153}.
CC -!- SIMILARITY: Belongs to the SPC24 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF07836.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC010871; AAF07836.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE74690.1; -; Genomic_DNA.
DR EMBL; AK175522; BAD43285.1; -; mRNA.
DR EMBL; AK176735; BAD44498.1; -; mRNA.
DR EMBL; BT010497; AAQ65120.1; -; mRNA.
DR RefSeq; NP_187500.2; NM_111722.3.
DR AlphaFoldDB; Q67XT3; -.
DR SMR; Q67XT3; -.
DR IntAct; Q67XT3; 1.
DR STRING; 3702.AT3G08880.1; -.
DR PaxDb; Q67XT3; -.
DR PRIDE; Q67XT3; -.
DR ProteomicsDB; 232615; -.
DR DNASU; 820036; -.
DR EnsemblPlants; AT3G08880.1; AT3G08880.1; AT3G08880.
DR GeneID; 820036; -.
DR Gramene; AT3G08880.1; AT3G08880.1; AT3G08880.
DR KEGG; ath:AT3G08880; -.
DR Araport; AT3G08880; -.
DR TAIR; locus:2097603; AT3G08880.
DR eggNOG; ENOG502S0WN; Eukaryota.
DR HOGENOM; CLU_117840_0_0_1; -.
DR InParanoid; Q67XT3; -.
DR OMA; MYASITN; -.
DR OrthoDB; 1450813at2759; -.
DR PhylomeDB; Q67XT3; -.
DR PRO; PR:Q67XT3; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q67XT3; baseline and differential.
DR GO; GO:0000776; C:kinetochore; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0009933; P:meristem structural organization; IMP:TAIR.
DR GO; GO:0051781; P:positive regulation of cell division; IMP:TAIR.
DR GO; GO:0051983; P:regulation of chromosome segregation; IMP:TAIR.
DR InterPro; IPR044951; SPC24-like.
DR PANTHER; PTHR35730; PTHR35730; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Mitosis;
KW Reference proteome.
FT CHAIN 1..201
FT /note="Kinetochore protein SPC24 homolog"
FT /id="PRO_0000444101"
FT COILED 78..133
FT /evidence="ECO:0000255"
SQ SEQUENCE 201 AA; 23332 MW; 2137FCC96935E76C CRC64;
MGNASENFDI EDLMSYGDDL INLLNVKNGF DIISQSSEQF KALNFACHED FNQIQGSIED
CKTKLYACKK KTEEAYSDIA AEDEIERLQK ELDEEMEREF KLKDELRLVA DELKDLNAQL
SSIDEHKQST KRKVRDDLRA EKKLSMYASV TNVIPDIDDP SKISGYMVDR EKRLIEKFQF
ETNKMTAYET CNSIWSIINK Q
