ID   SPC24_DANRE             Reviewed;         202 AA.
AC   Q503N2; Q6DG65;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   19-SEP-2006, sequence version 2.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Kinetochore protein Spc24;
GN   Name=spc24; Synonyms=spbc24; ORFNames=zgc:110417;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC       NDC80 complex, which is required for chromosome segregation and spindle
CC       checkpoint activity. Required for kinetochore integrity and the
CC       organization of stable microtubule binding sites in the outer plate of
CC       the kinetochore. The NDC80 complex synergistically enhances the
CC       affinity of the SKA1 complex for microtubules and may allow the NDC80
CC       complex to track depolymerizing microtubules.
CC       {ECO:0000250|UniProtKB:Q8NBT2}.
CC   -!- SUBUNIT: Component of the NDC80 complex, which is composed of ndc80,
CC       cdca1, spbc24 and spbc25. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8NBT2}.
CC       Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:Q8NBT2}.
CC       Note=Localizes to kinetochores from late prophase to anaphase.
CC       Localizes specifically to the outer plate of the kinetochore.
CC       {ECO:0000250|UniProtKB:Q8NBT2}.
CC   -!- SIMILARITY: Belongs to the SPC24 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH76486.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; BC076486; AAH76486.1; ALT_INIT; mRNA.
DR   EMBL; BC095251; AAH95251.1; -; mRNA.
DR   RefSeq; NP_001019611.1; NM_001024440.1.
DR   RefSeq; XP_017209818.1; XM_017354329.1.
DR   AlphaFoldDB; Q503N2; -.
DR   SMR; Q503N2; -.
DR   STRING; 7955.ENSDARP00000059307; -.
DR   PaxDb; Q503N2; -.
DR   Ensembl; ENSDART00000059308; ENSDARP00000059307; ENSDARG00000093622.
DR   GeneID; 554147; -.
DR   KEGG; dre:554147; -.
DR   CTD; 147841; -.
DR   ZFIN; ZDB-GENE-050522-456; spc24.
DR   eggNOG; ENOG502S26V; Eukaryota.
DR   GeneTree; ENSGT00390000005584; -.
DR   HOGENOM; CLU_119584_0_0_1; -.
DR   InParanoid; Q503N2; -.
DR   OMA; KWEYDTQ; -.
DR   OrthoDB; 1602618at2759; -.
DR   PhylomeDB; Q503N2; -.
DR   TreeFam; TF333217; -.
DR   Reactome; R-DRE-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR   Reactome; R-DRE-2467813; Separation of Sister Chromatids.
DR   Reactome; R-DRE-2500257; Resolution of Sister Chromatid Cohesion.
DR   Reactome; R-DRE-5663220; RHO GTPases Activate Formins.
DR   Reactome; R-DRE-9648025; EML4 and NUDC in mitotic spindle formation.
DR   PRO; PR:Q503N2; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 3.
DR   Bgee; ENSDARG00000093622; Expressed in early embryo and 28 other tissues.
DR   ExpressionAtlas; Q503N2; baseline.
DR   GO; GO:0031262; C:Ndc80 complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; IBA:GO_Central.
DR   InterPro; IPR013252; Ndc80_Spc24.
DR   PANTHER; PTHR22142; PTHR22142; 1.
DR   Pfam; PF08286; Spc24; 1.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..202
FT                   /note="Kinetochore protein Spc24"
FT                   /id="PRO_0000249561"
FT   COILED          69..138
FT                   /evidence="ECO:0000255"
FT   CONFLICT        65
FT                   /note="E -> G (in Ref. 1; AAH95251)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   202 AA;  23398 MW;  52951162AD7F850F CRC64;
     MSHSISMHDD DLEKLGESLN EIIMSSKAES VLQAARKKKQ EFFDHYIKTK KTLTELLNDL
     VHTEEVVGQK LLDLDQQKSQ MMQEIMKMEQ EVQRSQSKSQ NLDSELEFLQ KELERLRDAE
     QEIQTLQQEV DEDTTEVIPS AIYVAQLYSK VTKIKLEFDT EPHILKGVHY GADVVTPINI
     DTSTRTPCEV SDELWSLVKT EW