ID   SPC24_XENLA             Reviewed;         199 AA.
AC   Q6NRW3;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Kinetochore protein Spc24;
DE            Short=xSpc24;
GN   Name=spc24; Synonyms=spbc24;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY,
RP   IDENTIFICATION IN THE NDC80 COMPLEX, AND SUBCELLULAR LOCATION.
RX   PubMed=14738735; DOI=10.1016/j.cub.2003.12.058;
RA   McCleland M.L., Kallio M.J., Barrett-Wilt G.A., Kestner C.A.,
RA   Shabanowitz J., Hunt D.F., Gorbsky G.J., Stukenberg P.T.;
RT   "The vertebrate Ndc80 complex contains Spc24 and Spc25 homologs, which are
RT   required to establish and maintain kinetochore-microtubule attachment.";
RL   Curr. Biol. 14:131-137(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Ovary;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   CHARACTERIZATION OF THE NDC80 COMPLEX, AND INTERACTION OF THE NDC80 COMPLEX
RP   WITH MIS12 AND ZWINT.
RX   PubMed=16079178; DOI=10.1091/mbc.e05-03-0239;
RA   Emanuele M.J., McCleland M.L., Satinover D.L., Stukenberg P.T.;
RT   "Measuring the stoichiometry and physical interactions between components
RT   elucidates the architecture of the vertebrate kinetochore.";
RL   Mol. Biol. Cell 16:4882-4892(2005).
CC   -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC       NDC80 complex, which is required for chromosome segregation and spindle
CC       checkpoint activity (PubMed:14738735). Required for kinetochore
CC       integrity and the organization of stable microtubule binding sites in
CC       the outer plate of the kinetochore (PubMed:14738735). The NDC80 complex
CC       synergistically enhances the affinity of the SKA1 complex for
CC       microtubules and may allow the NDC80 complex to track depolymerizing
CC       microtubules (By similarity). {ECO:0000250|UniProtKB:Q8NBT2,
CC       ECO:0000269|PubMed:14738735}.
CC   -!- SUBUNIT: Component of the NDC80 complex, which is composed of ndc80,
CC       cdca1, spbc24 and spbc25. The NDC80 complex interacts with mis12 and
CC       zwint. {ECO:0000269|PubMed:14738735, ECO:0000269|PubMed:16079178}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14738735}.
CC       Chromosome, centromere, kinetochore {ECO:0000269|PubMed:14738735}.
CC       Note=Localizes to kinetochores from late prophase to anaphase.
CC       {ECO:0000269|PubMed:14738735}.
CC   -!- SIMILARITY: Belongs to the SPC24 family. {ECO:0000305}.
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DR   EMBL; AY456385; AAR88649.1; -; mRNA.
DR   EMBL; BC070597; AAH70597.1; -; mRNA.
DR   RefSeq; NP_001084436.1; NM_001090967.1.
DR   AlphaFoldDB; Q6NRW3; -.
DR   SMR; Q6NRW3; -.
DR   IntAct; Q6NRW3; 4.
DR   DNASU; 403385; -.
DR   GeneID; 403385; -.
DR   KEGG; xla:403385; -.
DR   CTD; 403385; -.
DR   Xenbase; XB-GENE-974565; spc24.L.
DR   OMA; KWEYDTQ; -.
DR   OrthoDB; 1602618at2759; -.
DR   Proteomes; UP000186698; Chromosome 3L.
DR   Bgee; 403385; Expressed in blastula and 19 other tissues.
DR   GO; GO:0031262; C:Ndc80 complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   InterPro; IPR013252; Ndc80_Spc24.
DR   PANTHER; PTHR22142; PTHR22142; 1.
DR   Pfam; PF08286; Spc24; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..199
FT                   /note="Kinetochore protein Spc24"
FT                   /id="PRO_0000249562"
FT   COILED          45..134
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   199 AA;  22722 MW;  0E49B6BD9F03D46A CRC64;
     MLCEQISEYV DVSREIVKVM VSDSAAGALK KSLDRQEAMI DSLLDTEVQA SQLIRDLMAV
     EEKVAQKLLD TEETKQKSSS KLQKIDRELQ ERMEKNASLE SSIKFLQKDL EELKVMEEEI
     ADMQREADED TTTVIPSAVY LAKLFHNVTK IDWDYNCDPS LIKGIHYGGD IAQPISIDSN
     QHSKIFICNY LWSLLSTDW