SPC25_ARATH
ID SPC25_ARATH Reviewed; 315 AA.
AC Q93VK9; Q8LAK6; Q9STR7;
DT 23-MAY-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Kinetochore protein SPC25 homolog {ECO:0000303|PubMed:29356153};
GN Name=SPC25 {ECO:0000303|PubMed:29356153};
GN OrderedLocusNames=At3g48210 {ECO:0000312|Araport:AT3G48210};
GN ORFNames=T24C20_90 {ECO:0000312|EMBL:CAB51068.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [6]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=29356153; DOI=10.1111/tpj.13823;
RA Shin J., Jeong G., Park J.Y., Kim H., Lee I.;
RT "MUN (MERISTEM UNSTRUCTURED), encoding a SPC24 homolog of NDC80 kinetochore
RT complex, affects development through cell division in Arabidopsis
RT thaliana.";
RL Plant J. 93:977-991(2018).
CC -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC NDC80 complex, which is required for chromosome segregation and spindle
CC checkpoint activity to ensure proper cell division.
CC {ECO:0000305|PubMed:29356153}.
CC -!- SUBUNIT: Component of the NDC80 complex, which consists of NDC80, NUF2,
CC SPC24 and SPC25. {ECO:0000269|PubMed:29356153}.
CC -!- SUBCELLULAR LOCATION: Chromosome, centromere
CC {ECO:0000269|PubMed:29356153}.
CC -!- SIMILARITY: Belongs to the SPC25 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM65290.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAB51068.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL096856; CAB51068.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE78385.1; -; Genomic_DNA.
DR EMBL; AF370157; AAK43972.1; -; mRNA.
DR EMBL; AY059099; AAL15205.1; -; mRNA.
DR EMBL; AY087754; AAM65290.1; ALT_INIT; mRNA.
DR PIR; T13010; T13010.
DR RefSeq; NP_566900.1; NM_114691.4.
DR AlphaFoldDB; Q93VK9; -.
DR IntAct; Q93VK9; 1.
DR STRING; 3702.AT3G48210.1; -.
DR iPTMnet; Q93VK9; -.
DR PaxDb; Q93VK9; -.
DR PRIDE; Q93VK9; -.
DR ProteomicsDB; 228289; -.
DR EnsemblPlants; AT3G48210.1; AT3G48210.1; AT3G48210.
DR GeneID; 823978; -.
DR Gramene; AT3G48210.1; AT3G48210.1; AT3G48210.
DR KEGG; ath:AT3G48210; -.
DR Araport; AT3G48210; -.
DR TAIR; locus:2100479; AT3G48210.
DR eggNOG; KOG4657; Eukaryota.
DR HOGENOM; CLU_069238_0_0_1; -.
DR InParanoid; Q93VK9; -.
DR OMA; TMREKFQ; -.
DR OrthoDB; 1460234at2759; -.
DR PhylomeDB; Q93VK9; -.
DR PRO; PR:Q93VK9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q93VK9; baseline and differential.
DR GO; GO:0031262; C:Ndc80 complex; IBA:GO_Central.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IBA:GO_Central.
DR InterPro; IPR045143; Spc25.
DR InterPro; IPR013255; Spc25_C.
DR PANTHER; PTHR14281; PTHR14281; 1.
DR Pfam; PF08234; Spindle_Spc25; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cell cycle; Cell division; Centromere; Chromosome;
KW Coiled coil; Mitosis; Reference proteome.
FT CHAIN 1..315
FT /note="Kinetochore protein SPC25 homolog"
FT /id="PRO_0000444104"
FT REGION 261..315
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 57..91
FT /evidence="ECO:0000255"
FT COMPBIAS 261..279
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CONFLICT 87
FT /note="R -> C (in Ref. 4; AAM65290)"
FT /evidence="ECO:0000305"
FT CONFLICT 136
FT /note="K -> T (in Ref. 4; AAM65290)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 315 AA; 35578 MW; A3552C4D1CACE84E CRC64;
MEQISNIAGG DTTKETMASL GLICEKDIHE QRLKIDSFIA SPFRRSMNSL VERAQATAQS
QVELMNLKAD LREAEDELVK VLAVKTRKEA RQMGIRDSIS ATQSRIEVLR RNLQLQKSKK
DDSVRIISQQ LQALSKSKDN AGKVTEDKAD IHEAISWYNH ALGFHVEAGH GVKFTFTNID
AKRPTREFSF TVHYGNDIYT LLDSDLQLDY INEMVQELNK TNDLFRFVRL MREQFLKSTL
SELPTHSGQL QQETSAISAS APAISFSTDT NMSTPENKRS KVQVNRRQKR GSESPLLAPV
STSATRRSSR FKGKK
