ID   SPC25_ASHGO             Reviewed;         231 AA.
AC   Q751Z6;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Probable kinetochore protein SPC25;
GN   Name=SPC25; OrderedLocusNames=AFR679C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC       NDC80 complex, which is required for chromosome segregation and spindle
CC       checkpoint activity. {ECO:0000250}.
CC   -!- SUBUNIT: Component of the NDC80 complex, which consists of NDC80, NUF2,
CC       SPC24 and SPC25. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P40014}.
CC       Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:P40014}.
CC       Note=Associated with kinetochores. {ECO:0000250|UniProtKB:P40014}.
CC   -!- SIMILARITY: Belongs to the SPC25 family. {ECO:0000305}.
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DR   EMBL; AE016819; AAS54051.1; -; Genomic_DNA.
DR   RefSeq; NP_986227.1; NM_212363.1.
DR   AlphaFoldDB; Q751Z6; -.
DR   SMR; Q751Z6; -.
DR   STRING; 33169.AAS54051; -.
DR   EnsemblFungi; AAS54051; AAS54051; AGOS_AFR679C.
DR   GeneID; 4622516; -.
DR   KEGG; ago:AGOS_AFR679C; -.
DR   eggNOG; KOG4657; Eukaryota.
DR   HOGENOM; CLU_085127_1_0_1; -.
DR   InParanoid; Q751Z6; -.
DR   OMA; HEDQRMK; -.
DR   Proteomes; UP000000591; Chromosome VI.
DR   GO; GO:0031262; C:Ndc80 complex; IEA:InterPro.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; IEA:InterPro.
DR   InterPro; IPR045143; Spc25.
DR   InterPro; IPR013255; Spc25_C.
DR   PANTHER; PTHR14281; PTHR14281; 1.
DR   Pfam; PF08234; Spindle_Spc25; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..231
FT                   /note="Probable kinetochore protein SPC25"
FT                   /id="PRO_0000246669"
FT   COILED          23..132
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   231 AA;  26094 MW;  D1EE00E65433E240 CRC64;
     MSLEGFAELR AQMEAFQGRV HGHLGQQRHA MEQLLAAHRQ EVEKLRLRTG AVQQQLARLA
     GEEQELRQNI SSLQERTQHT TAQRDAYAAQ RQKLAAERTQ LEHDRAELHA MLARKQAELQ
     RQREAIKRQH MRDNPEVRVY EQLLGLYVRA SRPDSLEFAF RNVSEADARA ECSFTLDLGA
     DGYRVAAAQP PLPLERLRDL ERDLGSSGDL PAFLKAIRAA LVTAMAGQAD A