ID   SPC25_DROLT             Reviewed;         223 AA.
AC   Q64EV9;
DT   23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 29.
DE   RecName: Full=Kinetochore protein Spc25 {ECO:0000250|UniProtKB:Q9V3V7};
GN   Name=Spc25 {ECO:0000250|UniProtKB:Q9V3V7};
GN   Synonyms=mitch {ECO:0000312|EMBL:AAU15008.1,
GN   ECO:0000312|FlyBase:FBgn0082543};
OS   Drosophila lutescens (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=51159;
RN   [1] {ECO:0000312|EMBL:AAU15008.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=17895365; DOI=10.1242/jcs.012112;
RA   Williams B., Leung G., Maiato H., Wong A., Li Z., Williams E.V.,
RA   Kirkpatrick C., Aquadro C.F., Rieder C.L., Goldberg M.L.;
RT   "Mitch a rapidly evolving component of the Ndc80 kinetochore complex
RT   required for correct chromosome segregation in Drosophila.";
RL   J. Cell Sci. 120:3522-3533(2007).
CC   -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC       Ndc80 complex, which is required for chromosome segregation and spindle
CC       checkpoint activity during meiosis and mitosis. Required for
CC       kinetochore integrity and the organization of stable microtubule
CC       binding sites in the outer plate of the kinetochore. Participates in
CC       SAC signaling that responds specifically to disruptions in spindle
CC       microtubule dynamics. The NDC80 complex synergistically enhances the
CC       affinity of the SKA1 complex for microtubules and may allow the NDC80
CC       complex to track depolymerizing microtubules.
CC       {ECO:0000250|UniProtKB:Q9HBM1, ECO:0000250|UniProtKB:Q9V3V7}.
CC   -!- SUBUNIT: Component of the Ndc80 complex, which is composed of Ndc80,
CC       Nuf2 and Spc25. {ECO:0000250|UniProtKB:Q9V3V7}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9V3V7}.
CC       Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:Q9V3V7}.
CC   -!- SIMILARITY: Belongs to the SPC25 family. {ECO:0000255}.
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DR   EMBL; AY714314; AAU15008.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q64EV9; -.
DR   SMR; Q64EV9; -.
DR   FlyBase; FBgn0082543; Dlut\mitch.
DR   GO; GO:0031262; C:Ndc80 complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0051311; P:meiotic metaphase plate congression; ISS:UniProtKB.
DR   GO; GO:0000212; P:meiotic spindle organization; ISS:UniProtKB.
DR   GO; GO:0007080; P:mitotic metaphase plate congression; ISS:UniProtKB.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Meiosis; Mitosis; Nucleus.
FT   CHAIN           1..223
FT                   /note="Kinetochore protein Spc25"
FT                   /id="PRO_0000392418"
FT   COILED          65..115
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   223 AA;  25447 MW;  9B08DCFD19C76E28 CRC64;
     MAKAVDESTY EQRVRALYDK QIRMEAREAG VIKKISKFNS NLLDLKEAVV RHHQKVGKVR
     KVKMLRCGEL EKRANFMEEL TQELEATKQR NLVMRDQIKQ LNVLARQHRN EVMESIHTLS
     KTTGTYLNHE ALPARVKGVT VLRNDNSDQL IPFDLKATDV EGLESLCQHL QGFNIDAFQW
     RQLVSLATEM SMDSHTTPTT PPKEAAEGKS IIEIDLTSPT SHI