ID   SPC25_DROMA             Reviewed;         222 AA.
AC   Q64EW6;
DT   23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=Kinetochore protein Spc25 {ECO:0000250|UniProtKB:Q9V3V7};
GN   Name=Spc25 {ECO:0000250|UniProtKB:Q9V3V7};
GN   Synonyms=mitch {ECO:0000312|EMBL:AAU15001.1,
GN   ECO:0000312|FlyBase:FBgn0082531};
OS   Drosophila mauritiana (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7226;
RN   [1] {ECO:0000312|EMBL:AAU15001.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=17895365; DOI=10.1242/jcs.012112;
RA   Williams B., Leung G., Maiato H., Wong A., Li Z., Williams E.V.,
RA   Kirkpatrick C., Aquadro C.F., Rieder C.L., Goldberg M.L.;
RT   "Mitch a rapidly evolving component of the Ndc80 kinetochore complex
RT   required for correct chromosome segregation in Drosophila.";
RL   J. Cell Sci. 120:3522-3533(2007).
CC   -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC       Ndc80 complex, which is required for chromosome segregation and spindle
CC       checkpoint activity during meiosis and mitosis. Required for
CC       kinetochore integrity and the organization of stable microtubule
CC       binding sites in the outer plate of the kinetochore. Participates in
CC       SAC signaling that responds specifically to disruptions in spindle
CC       microtubule dynamics. The NDC80 complex synergistically enhances the
CC       affinity of the SKA1 complex for microtubules and may allow the NDC80
CC       complex to track depolymerizing microtubules.
CC       {ECO:0000250|UniProtKB:Q9HBM1, ECO:0000250|UniProtKB:Q9V3V7}.
CC   -!- SUBUNIT: Component of the Ndc80 complex, which is composed of Ndc80,
CC       Nuf2 and Spc25. {ECO:0000250|UniProtKB:Q9V3V7}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9V3V7}.
CC       Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:Q9V3V7}.
CC   -!- SIMILARITY: Belongs to the SPC25 family. {ECO:0000255}.
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DR   EMBL; AY714307; AAU15001.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q64EW6; -.
DR   FlyBase; FBgn0082531; Dmau\mitch.
DR   GO; GO:0031262; C:Ndc80 complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0051311; P:meiotic metaphase plate congression; ISS:UniProtKB.
DR   GO; GO:0000212; P:meiotic spindle organization; ISS:UniProtKB.
DR   GO; GO:0007080; P:mitotic metaphase plate congression; ISS:UniProtKB.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Meiosis; Mitosis; Nucleus.
FT   CHAIN           1..222
FT                   /note="Kinetochore protein Spc25"
FT                   /id="PRO_0000392419"
FT   COILED          51..86
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   222 AA;  25619 MW;  E7F2A9BADA48C9A6 CRC64;
     MAIIMAESSY ERRVKALYEK QIRMEALEAK FIKKVYKFNS NLLDVKEAAC RHQRKVGKLQ
     KVLMERREEL DKRVSFIEEL DRELEATKLR DLAMKDRIKQ QKMLARQRKN EIMESIHTLS
     KTTGTYINQD ALPARVKGVT VLRGDKRNQL IPFDLKSTDV EGLDSLCQHL ESLNVDMAQW
     QQLISLAMDV AMESRAPTTP PKEATNCNSI IEIDLTSPTC HI